KIAA0494 (EFCAB14) (NM_014774) Human Tagged ORF Clone

CAT#: RC200565

EFCAB14 (Myc-DDK-tagged)-Human KIAA0494 (KIAA0494)

Product Images

Western blot validation of overexpression lysate (Cat# LY415039) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200565 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified EFCAB14 protein (Cat# TP300565). The protein was produced from HEK293T cells transfected with EFCAB14 cDNA clone (Cat# RC200565) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EFCAB14
• Synonyms: KIAA0494
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200565 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAAAGCGCAAAGAGCTCAATGCATTGATTGGTTTGGCTGGGGACAGCCGGAGAAAGAAGCCCAAGA
  AAGGCCCAAGCAGTCACCGCCTGCTTCGCACTGAGCCTCCCGACTCAGACTCTGAGTCCAGCTCCGAAGA
  GGAAGAGGAATTCGGTGTGGTTGGAAATCGCTCTCGCTTTGCCAAGGGAGACTATTTACGATGCTGCAAG
  ATCTGTTATCCGCTCTGTGGTTTTGTCATCCTTGCTGCCTGTGTTGTGGCCTGTGTTGGCTTGGTGTGGA
  TGCAGGTTGCTCTCAAGGAGGATCTGGATGCCCTCAAGGAAAAATTTCGAACAATGGAATCTAATCAGAA
  AAGCTCATTCCAAGAAATCCCCAAACTTAATGAAGAACTACTCAGCAAGCAAAAACAACTTGAGAAGATT
  GAATCTGGAGAGATGGGTTTGAACAAAGTCTGGATAAACATCACAGAAATGAATAAGCAGATTTCTCTGT
  TGACTTCTGCAGTGAACCACCTCAAAGCCAATGTTAAGTCAGCTGCAGACTTGATTAGCCTGCCTACCAC
  TGTAGAGGGACTTCAGAAGAGTGTAGCTTCCATTGGCAATACTTTAAACAGCGTCCATCTTGCTGTGGAA
  GCACTACAGAAAACTGTGGATGAACACAAGAAAACGATGGAATTACTGCAGAGTGATATGAATCAGCACT
  TCTTGAAGGAGACTCCTGGAAGCAACCAGATCATTCCGTCACCTTCAGCCACATCAGAACTTGACAATAA
  AACCCACAGTGAGAATTTGAAACAGGATATCCTGTACCTTCACAACTCTTTAGAGGAGGTAAACAGTGCC
  CTAGTGGGGTACCAGAGACAGAATGATCTTAAACTCGAGGGAATGAACGAGACAGTCAGTAATCTTACCC
  AGAGAGTCAACCTGATAGAAAGCGATGTGGTTGCTATGAGCAAGGTAGAAAAGAAAGCAAACCTGTCCTT
  CAGCATGATGGGTGATAGATCTGCCACTCTGAAAAGACAGTCTTTGGATCAAGTCACCAACAGAACAGAT
  ACAGTAAAAATCCAAAGCATAAAGAAAGAAGATAGTTCAAATTCTCAGGTATCCAAGCTAAGAGAGAAAC
  TCCAGCTGATCAGTGCTCTTACAAACAAACCTGAGAGCAACAGGCCTCCAGAGACCGCCGATGAAGAGCA
  AGTAGAGAGTTTCACATCAAAGCCATCAGCATTGCCAAAATTTTCACAGTTTCTTGGAGACCCAGTTGAG
  AAAGCTGCCCAACTAAGACCTATCTCCCTACCAGGAGTTTCTAGCACTGAAGATCTTCAGGATTTATTCC
  GCAAGACTGGCCAGGACGTGGATGGGAAGCTGACCTACCAGGAAATCTGGACCTCCCTAGGTTCTGCTAT
  GCCAGAACCAGAGAGCTTGAGAGCATTTGATTCCGATGGAGATGGAAGATACTCATTCCTGGAGCTAAGG
  GTAGCTTTAGGTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200565 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKKRKELNALIGLAGDSRRKKPKKGPSSHRLLRTEPPDSDSESSSEEEEEFGVVGNRSRFAKGDYLRCCK
  ICYPLCGFVILAACVVACVGLVWMQVALKEDLDALKEKFRTMESNQKSSFQEIPKLNEELLSKQKQLEKI
  ESGEMGLNKVWINITEMNKQISLLTSAVNHLKANVKSAADLISLPTTVEGLQKSVASIGNTLNSVHLAVE
  ALQKTVDEHKKTMELLQSDMNQHFLKETPGSNQIIPSPSATSELDNKTHSENLKQDILYLHNSLEEVNSA
  LVGYQRQNDLKLEGMNETVSNLTQRVNLIESDVVAMSKVEKKANLSFSMMGDRSATLKRQSLDQVTNRTD
  TVKIQSIKKEDSSNSQVSKLREKLQLISALTNKPESNRPPETADEEQVESFTSKPSALPKFSQFLGDPVE
  KAAQLRPISLPGVSSTEDLQDLFRKTGQDVDGKLTYQEIWTSLGSAMPEPESLRAFDSDGDGRYSFLELR
  VALGI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014774
• ORF Size: 1485 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014774.1, NM_014774.2, NP_055589.1
• RefSeq Size: 5794 bp
• RefSeq ORF: 1488 bp
• Locus ID: 9813
• Protein Families: Transmembrane
• MW: 55 kDa