HIV TAT specific factor 1 (HTATSF1) (NM_014500) Human Tagged ORF Clone

CAT#: RC200601

HTATSF1 (Myc-DDK-tagged)-Human HIV-1 Tat specific factor 1 (HTATSF1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HTATSF1
• Synonyms: dJ196E23.2; TAT-SF1; TATSF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200601 representing NM_014500
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGCGGCACCAACTTGGATGGGAACGATGAGTTTGATGAGCAGTTGCGAATGCAAGAATTGTACGGAG
  ACGGCAAGGATGGTGACACCCAGACCGATGCCGGCGGAGAACCCGATTCTCTCGGGCAGCAGCCGACGGA
  CACTCCCTACGAGTGGGACCTGGACAAAAAGGCTTGGTTCCCCAAGATTACTGAAGATTTCATTGCTACA
  TATCAGGCCAATTATGGCTTCTCTAACGATGGCGCATCTAGTTCTACCGCAAATGTTGAAGATGTCCATG
  CTAGGACTGCAGAGGAACCTCCACAAGAAAAAGCCCCGGAACCCACTGATGCCAGAAAGAAGGGAGAAAA
  AAGAAAGGCTGAGTCAGGATGGTTTCATGTTGAAGAAGACAGAAATACAAATGTATACGTGTCTGGTTTG
  CCTCCAGATATTACAGTGGATGAATTTATACAACTTATGTCCAAGTTTGGCATTATTATGAGAGATCCTC
  AGACAGAAGAATTTAAGGTCAAACTTTACAAAGATAATCAAGGAAATCTTAAAGGAGACGGTCTTTGCTG
  TTATTTGAAAAGAGAATCTGTGGAACTTGCATTAAAACTTTTGGATGAAGATGAAATTAGAGGCTACAAA
  TTACATGTTGAGGTGGCAAAGTTTCAACTGAAGGGAGAATATGATGCCTCAAAGAAGAAGAAGAAGTGCA
  AAGACTATAAGAAGAAGCTGTCTATGCAACAAAAGCAGTTGGATTGGAGACCTGAGAGGCGAGCCGGACC
  ATCCCGGATGCGCCATGAGCGAGTTGTCATCATCAAGAATATGTTTCATCCTATGGATTTTGAGGATGAT
  CCGTTGGTGCTGAATGAGATCAGAGAAGACCTTCGAGTAGAGTGTTCGAAGTTTGGACAAATTAGGAAAC
  TCCTTCTCTTTGATAGGCACCCAGATGGTGTGGCCTCTGTGTCCTTTCGGGATCCAGAGGAAGCTGATTA
  TTGTATTCAGACTCTCGATGGAAGATGGTTTGGTGGCCGTCAAATCACTGCCCAGGCATGGGATGGGACT
  ACAGATTATCAGGTGGAGGAAACCTCAAGAGAAAGGGAGGAAAGGCTGAGAGGATGGGAGGCTTTCCTCA
  ATGCTCCTGAGGCCAACAGAGGCCTTAGGCGTTCAGATTCTGTCTCTGCTTCCGAAAGGGCAGGGCCTTC
  TAGAGCAAGGCATTTTTCAGAGCACCCCAGCACATCTAAAATGAATGCTCAAGAAACTGCAACTGGAATG
  GCGTTTGAAGAACCTATAGATGAGAAGAAGTTTGAAAAGACAGAAGATGGGGGAGAATTTGAAGAAGGTG
  CTTCTGAAAACAATGCTAAGGAAAGTAGCCCCGAAAAAGAGGCTGAAGAAGGCTGCCCTGAAAAAGAATC
  TGAAGAGGGCTGCCCCAAAAGAGGGTTTGAAGGCAGCTGCTCCCAAAAAGAGTCTGAAGAAGGCAATCCC
  GTAAGAGGATCTGAAGAGGATAGTCCTAAAAAAGAGTCTAAAAAGAAGACACTCAAAAATGATTGTGAAG
  AGAATGGCCTTGCAAAGGAATCTGAAGATGACCTCAACAAGGAGTCTGAAGAGGAGGTTGGCCCCACAAA
  AGAGTCCGAAGAAGATGACTCAGAGAAAGAGTCTGATGAAGACTGCTCTGAAAAACAGTCTGAAGATGGC
  TCCGAAAGAGAATTTGAAGAAAATGGTCTCGAGAAAGATTTGGACGAGGAAGGTTCTGAAAAGGAGCTTC
  ATGAAAATGTTCTTGACAAAGAGTTAGAAGAAAATGACTCTGAAAACTCCGAATTTGAAGATGACGGCTC
  TGAAAAAGTGTTAGATGAGGAAGGCTCTGAGAGAGAGTTTGACGAAGATTCAGATGAAAAGGAAGAAGAG
  GAGGATACATATGAAAAAGTATTTGATGATGAGTCTGATGAGAAAGAGGATGAAGAATATGCAGATGAAA
  AGGGGCTTGAAGCTGCTGATAAAAAGGCGGAAGAAGGTGATGCAGATGAAAAGCTGTTTGAAGAGTCAGA
  TGACAAGGAAGATGAAGATGCAGATGGAAAGGAAGTTGAAGATGCTGACGAAAAGTTGTTCGAAGATGAT
  GATTCCAATGAGAAGTTGTTTGATGAGGAGGAAGATTCCAGTGAGAAGTTGTTTGACGATTCTGATGAGA
  GGGGGACTTTGGGTGGTTTTGGGAGTGTTGAAGAAGGGCCCCTATCCACTGGCAGCAGCTTTATTCTCAG
  TAGCGATGATGATGACGATGATATT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200601 representing NM_014500
  Red=Cloning site Green=Tags(s)
  
  MSGTNLDGNDEFDEQLRMQELYGDGKDGDTQTDAGGEPDSLGQQPTDTPYEWDLDKKAWFPKITEDFIAT
  YQANYGFSNDGASSSTANVEDVHARTAEEPPQEKAPEPTDARKKGEKRKAESGWFHVEEDRNTNVYVSGL
  PPDITVDEFIQLMSKFGIIMRDPQTEEFKVKLYKDNQGNLKGDGLCCYLKRESVELALKLLDEDEIRGYK
  LHVEVAKFQLKGEYDASKKKKKCKDYKKKLSMQQKQLDWRPERRAGPSRMRHERVVIIKNMFHPMDFEDD
  PLVLNEIREDLRVECSKFGQIRKLLLFDRHPDGVASVSFRDPEEADYCIQTLDGRWFGGRQITAQAWDGT
  TDYQVEETSREREERLRGWEAFLNAPEANRGLRRSDSVSASERAGPSRARHFSEHPSTSKMNAQETATGM
  AFEEPIDEKKFEKTEDGGEFEEGASENNAKESSPEKEAEEGCPEKESEEGCPKRGFEGSCSQKESEEGNP
  VRGSEEDSPKKESKKKTLKNDCEENGLAKESEDDLNKESEEEVGPTKESEEDDSEKESDEDCSEKQSEDG
  SEREFEENGLEKDLDEEGSEKELHENVLDKELEENDSENSEFEDDGSEKVLDEEGSEREFDEDSDEKEEE
  EDTYEKVFDDESDEKEDEEYADEKGLEAADKKAEEGDADEKLFEESDDKEDEDADGKEVEDADEKLFEDD
  DSNEKLFDEEEDSSEKLFDDSDERGTLGGFGSVEEGPLSTGSSFILSSDDDDDDI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014500
• ORF Size: 2265 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_014500.1, NM_014500.2, NM_014500.3, NM_014500.4, NP_055315.2
• RefSeq Size: 2788
• RefSeq ORF: 2268
• Locus ID: 27336
• Domains: RRM
• Protein Families: Transcription Factors
• MW: 86.3 kDa
• Gene Summary: The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]