ABHD14A (NM_015407) Human Tagged ORF Clone

CAT#: RC200785

ABHD14A (Myc-DDK-tagged)-Human abhydrolase domain containing 14A (ABHD14A)

Product Images

Western blot validation of overexpression lysate (Cat# LY414572) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200785 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified ABHD14A protein (Cat# TP300785). The protein was produced from HEK293T cells transfected with ABHD14A cDNA clone (Cat# RC200785) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ABHD14A
• Synonyms: DORZ1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200785 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCGGGGCGCTGTGCGGCTGCTGGTTCCGCCTGGGCGGGGCCCGCCCGCTCATCCCGTTGGGCCCGA
  CTGTGGTACAGACCTCCATGAGCCAGTCCCAGGTAGCCCTGCTGGGCCTGAGTCTGCTGCTCATGCTCCT
  ACTGTATGTGGGGCTGCCAGGCCCCCCTGAGCAGACTTCCTGCCTCTGGGGAGACCCCAATGTCACAGTC
  CTGGCTGGTCTCACCCCTGGCAACTCGCCCATCTTTTACCGCGAGGTGCTCCCACTCAACCAGGCACACA
  GGGTGGAGGTGGTGCTGCTTCATGGAAAGGCCTTTAACTCTCACACGTGGGAGCAGCTGGGCACACTGCA
  GCTACTGTCACAGAGGGGCTACCGGGCCGTGGCCCTTGACCTTCCAGGTTTTGGGAACTCGGCACCTTCA
  AAGGAGGCAAGCACAGAGGCAGGGCGGGCAGCGCTGCTGGAGCGGGCGCTGCGGGACCTGGAGGTACAGA
  ATGCCGTGTTGGTGAGCCCCTCGCTGAGTGGCCACTATGCCCTGCCCTTCCTGATGCGAGGCCACCACCA
  GCTACATGGATTTGTGCCCATCGCACCCACCTCCACCCAGAACTACACCCAGGAGCAATTCTGGGCTGTG
  AAGACTCCAACCCTTATCCTGTATGGAGAGCTGGACCACATCCTGGCTCGAGAGTCACTGCGGCAGCTCC
  GCCACCTGCCCAACCACTCTGTGGTGAAGCTACGCAATGCAGGCCATGCCTGTTACCTCCACAAGCCGCA
  AGACTTCCACCTTGTCCTGCTTGCCTTCCTTGACCATCTACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200785 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVGALCGCWFRLGGARPLIPLGPTVVQTSMSQSQVALLGLSLLLMLLLYVGLPGPPEQTSCLWGDPNVTV
  LAGLTPGNSPIFYREVLPLNQAHRVEVVLLHGKAFNSHTWEQLGTLQLLSQRGYRAVALDLPGFGNSAPS
  KEASTEAGRAALLERALRDLEVQNAVLVSPSLSGHYALPFLMRGHHQLHGFVPIAPTSTQNYTQEQFWAV
  KTPTLILYGELDHILARESLRQLRHLPNHSVVKLRNAGHACYLHKPQDFHLVLLAFLDHLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015407
• ORF Size: 813 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015407.1, NM_015407.2, NM_015407.3, NM_015407.4, NP_056222.1
• RefSeq Size: 1118 bp
• RefSeq ORF: 816 bp
• Locus ID: 25864
• MW: 29.7 kDa