ZNF226 (NM_001032372) Human Tagged ORF Clone

CAT#: RC201024

ZNF226 (Myc-DDK-tagged)-Human zinc finger protein 226 (ZNF226), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY422312) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223775 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF226
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201024 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAATATGTTCAAGGAAGCAGTGACCTTCAAGGACGTGGCTGTGGCCTTCACGGAGGAGGAATTGGGGC
  TGCTGGGCCCTGCCCAGAGGAAGCTGTACCGAGATGTGATGGTGGAGAACTTTAGGAACCTGCTGTCAGT
  GGGGCATCCACCCTTCAAACAAGATGTATCACCTATAGAAAGAAATGAGCAGCTTTGGATAATGACGACA
  GCAACCCGAAGACAGGGAAATTTAGGAGAGAAAAATCAAAGTAAGTTAATTACTGTTCAAGACAGAGAAT
  CAGAAGAAGAGCTTTCTTGTTGGCAAATCTGGCAACAAATTGCAAATGACTTAACCAGGTGTCAAGACTC
  CATGATCAATAATTCTCAGTGTCACAAACAAGGTGATTTCCCTTACCAGGTAGGGACAGAACTGTCTATT
  CAAATTTCTGAAGATGAGAACTATATAGTAAATAAAGCAGATGGTCCCAATAATACTGGGAATCCAGAGT
  TTCCTATCTTGAGAACCCAGGATTCTTGGAGGAAAACATTCCTGACTGAGTCACAGAGATTGAACAGAGA
  TCAGCAAATTTCCATAAAAAATAAATTATGTCAATGTAAGAAGGGTGTTGATCCCATCGGTTGGATTTCA
  CATCATGATGGTCATAGAGTACACAAAAGTGAAAAATCTTATAGACCCAATGATTATGAAAAAGACAACA
  TGAAGATTTTGACATTTGATCACAATAGCATGATTCACACAGGACAGAAATCGTACCAGTGTAATGAGTG
  TAAAAAACCCTTCAGTGATCTCTCCAGCTTTGATCTTCATCAGCAGTTACAATCAGGAGAGAAGTCTCTT
  ACATGTGTTGAGCGTGGAAAAGGCTTCTGTTACAGCCCAGTTCTTCCTGTTCATCAGAAAGTACATGTGG
  GAGAAAAACTTAAGTGTGATGAGTGTGGTAAGGAATTCAGTCAGGGCGCTCATCTACAGACCCATCAGAA
  AGTCCACGTGATAGAGAAACCATACAAATGTAAGCAATGTGGGAAAGGTTTCAGTCGTAGATCAGCACTT
  AATGTTCATTGCAAGGTCCACACGGCAGAGAAACCTTATAATTGTGAGGAGTGTGGGAGGGCCTTCAGTC
  AGGCCTCTCATCTTCAGGACCATCAGAGACTCCACACTGGGGAGAAGCCATTCAAATGTGATGCATGTGG
  TAAGAGCTTCAGTCGGAATTCACATCTTCAATCCCATCAAAGAGTTCATACAGGAGAGAAACCATACAAA
  TGTGAGGAGTGTGGTAAGGGCTTCATTTGTAGCTCAAATCTTTACATTCATCAGAGAGTCCACACAGGAG
  AAAAACCCTATAAATGTGAGGAATGTGGTAAAGGCTTTAGTCGGCCTTCAAGTCTTCAGGCCCATCAGGG
  AGTTCACACTGGAGAGAAGTCATACATATGTACTGTATGTGGGAAAGGCTTTACTCTGAGTTCAAATCTT
  CAAGCCCATCAGAGAGTCCACACTGGAGAGAAGCCATACAAATGCAATGAGTGTGGGAAGAGCTTCAGGA
  GGAATTCCCATTATCAAGTTCATCTAGTGGTCCACACAGGAGAGAAACCCTATAAATGTGAGATATGTGG
  GAAGGGCTTCAGTCAAAGTTCGTATCTTCAAATCCATCAGAAGGCCCACAGTATAGAGAAACCTTTTAAG
  TGTGAGGAGTGTGGGCAGGGTTTCAATCAGAGCTCACGACTTCAGATTCACCAGCTGATCCATACGGGTG
  AGAAACCATACAAATGTGAAGAGTGTGGCAAGGGATTTAGTCGTAGAGCAGATCTTAAAATTCACTGTAG
  GATCCACACAGGAGAGAAACCATATAATTGTGAGGAGTGTGGGAAGGTCTTCAGGCAGGCCTCAAATCTT
  TTGGCCCATCAGAGAGTCCACAGTGGAGAAAAACCATTCAAATGTGAAGAATGTGGGAAGAGTTTCGGTC
  GGAGTGCACATCTTCAAGCCCATCAAAAAGTCCACACTGGAGATAAGCCATACAAATGTGATGAGTGTGG
  GAAGGGCTTCAAGTGGAGCTTGAACCTTGACATGCATCAGAGGGTGCACACAGGAGAAAAACCATATAAA
  TGTGGGGAGTGTGGTAAGTACTTCAGTCAGGCCTCAAGTCTTCAACTTCATCAGAGTGTCCACACAGGAG
  AGAAACCATACAAATGTGATGTGTGTGGTAAAGTCTTCAGTCGGTCTTCACAACTACAGTCTCATCAGCG
  AGTTCACACTGGGGAGAAACCTTATAAATGTGAGATATGTGGTAAGAGCTTCAGTTGGCGATCAAATCTT
  ACAGTTCATCACAGAATCCATGTTGGTGATAAATCCTATAAAAGTAATAGGGGTGGTAAGAACATCAGAG
  AATCCACACAGGAAAAAAAATCTATAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201024 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MNMFKEAVTFKDVAVAFTEEELGLLGPAQRKLYRDVMVENFRNLLSVGHPPFKQDVSPIERNEQLWIMTT
  ATRRQGNLGEKNQSKLITVQDRESEEELSCWQIWQQIANDLTRCQDSMINNSQCHKQGDFPYQVGTELSI
  QISEDENYIVNKADGPNNTGNPEFPILRTQDSWRKTFLTESQRLNRDQQISIKNKLCQCKKGVDPIGWIS
  HHDGHRVHKSEKSYRPNDYEKDNMKILTFDHNSMIHTGQKSYQCNECKKPFSDLSSFDLHQQLQSGEKSL
  TCVERGKGFCYSPVLPVHQKVHVGEKLKCDECGKEFSQGAHLQTHQKVHVIEKPYKCKQCGKGFSRRSAL
  NVHCKVHTAEKPYNCEECGRAFSQASHLQDHQRLHTGEKPFKCDACGKSFSRNSHLQSHQRVHTGEKPYK
  CEECGKGFICSSNLYIHQRVHTGEKPYKCEECGKGFSRPSSLQAHQGVHTGEKSYICTVCGKGFTLSSNL
  QAHQRVHTGEKPYKCNECGKSFRRNSHYQVHLVVHTGEKPYKCEICGKGFSQSSYLQIHQKAHSIEKPFK
  CEECGQGFNQSSRLQIHQLIHTGEKPYKCEECGKGFSRRADLKIHCRIHTGEKPYNCEECGKVFRQASNL
  LAHQRVHSGEKPFKCEECGKSFGRSAHLQAHQKVHTGDKPYKCDECGKGFKWSLNLDMHQRVHTGEKPYK
  CGECGKYFSQASSLQLHQSVHTGEKPYKCDVCGKVFSRSSQLQSHQRVHTGEKPYKCEICGKSFSWRSNL
  TVHHRIHVGDKSYKSNRGGKNIRESTQEKKSIK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032372
• ORF Size: 2409 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032372.1, NP_001027544.1
• RefSeq Size: 2623 bp
• RefSeq ORF: 2412 bp
• Locus ID: 7769
• Cytogenetics: 19q13.31
• Protein Families: Transcription Factors
• MW: 91.9 kDa
• Gene Summary: May be involved in transcriptional regulation. [UniProtKB/Swiss-Prot Function]