valyl tRNA synthetase (VARS) (NM_006295) Human Tagged ORF Clone

CAT#: RC201089

VARS (Myc-DDK-tagged)-Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY416738) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201089 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified VARS protein (Cat# TP301089). The protein was produced from HEK293T cells transfected with VARS cDNA clone (Cat# RC201089) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: VARS
• Synonyms: G7A; NDMSCA; VARS1; VARS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201089 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACCCTCTACGTCTCCCCTCACCCAGATGCCTTCCCCAGCCTCCGAGCCCTCATAGCCGCTCGCT
  ATGGGGAGGCTGGGGAGGGTCCCGGATGGGGAGGAGCCCACCCCCGCATCTGTCTCCAGCCACCCCCGAC
  TAGCAGGACTAGCTTTCCCCCACCCCGCCTGCCGGCCCTGGAGCAGGGGCCCGGTGGGCTCTGGGTGTGG
  GGGGCCACGGCTGTGGCCCAGCTGCTGTGGCCAGCAGGCCTGGGGGGCCCAGGGGGCAGCCGGGCGGCTG
  TCCTTGTCCAACAGTGGGTCAGTTACGCCGACACGGAGTTAATACCAGCTGCCTGTGGAGCAACGCTGCC
  GGCCCTGGGACTCCGAAGCTCGGCCCAGGACCCCCAGGCTGTGCTGGGGGCCCTGGGCAGGGCCCTGAGC
  CCCTTGGAGGAGTGGCTTCGGCTGCACACCTACTTGGCCGGGGAGGCCCCCACTCTGGCTGACCTGGCGG
  CTGTCACAGCCTTGCTGCTGCCTTTCCGATACGTCCTAGACCCACCTGCCCGCCGGATCTGGAATAATGT
  GACTCGCTGGTTTGTCACGTGTGTCCGGCAGCCAGAATTCCGAGCCGTGCTAGGAGAAGTGGTTCTATAC
  TCAGGAGCCAGGCCTCTCTCTCATCAGCCAGGCCCCGAGGCTCCTGCCCTCCCAAAGACAGCTGCTCAGC
  TCAAGAAAGAGGCAAAGAAACGGGAGAAGCTAGAGAAATTCCAACAGAAGCAGAAGATCCAACAGCAGCA
  GCCACCTCCAGGGGAGAAGAAACCAAAACCAGAGAAGAGGGAGAAACGGGATCCTGGGGTCATTACCTAT
  GACCTCCCAACCCCACCCGGGGAAAAGAAAGATGTCAGTGGCCCCATGCCCGACTCCTACAGCCCTCGGT
  ATGTGGAGGCTGCCTGGTACCCTTGGTGGGAGCAGCAGGGCTTCTTCAAGCCAGAGTATGGGCGTCCTAA
  TGTGTCAGCAGCAAATCCCCGAGGTGTCTTCATGATGTGCATCCCACCCCCCAATGTGACAGGCTCCCTG
  CACCTGGGCCATGCACTCACCAACGCCATCCAGGACTCCCTGACTCGATGGCACCGCATGCGTGGGGAGA
  CCACCCTGTGGAACCCTGGCTGTGACCATGCAGGTATTGCCACCCAGGTGGTGGTGGAGAAGAAGCTATG
  GCGTGAGCAGGGACTGAGCCGGCACCAGCTGGGCCGCGAGGCCTTTCTACAGGAAGTCTGGAAGTGGAAG
  GAGGAGAAAGGTGACCGGATTTACCACCAGTTGAAGAAGCTTGGCAGCTCCTTGGACTGGGATCGAGCCT
  GTTTTACCATGGACCCTAAACTCTCAGCAGCTGTGACAGAGGCCTTTGTCCGGCTTCACGAGGAAGGCAT
  CATCTATCGCAGTACCCGCCTTGTTAACTGGTCCTGCACCCTCAACTCCGCCATCTCTGACATTGAGGTG
  GATAAGAAGGAGCTGACAGGTCGCACCCTGCTCTCCGTGCCTGGCTACAAGGAGAAGGTGGAGTTCGGGG
  TCCTCGTGTCCTTTGCCTATAAGGTCCAAGGCTCAGATAGCGACGAGGAGGTGGTGGTGGCAACAACTCG
  GATCGAGACAATGCTGGGAGATGTGGCTGTAGCTGTGCACCCCAAAGATACCAGATACCAGCACCTGAAG
  GGGAAGAACGTGATCCACCCATTCCTGTCTCGGAGCCTTCCCATTGTCTTCGATGAATTTGTGGACATGG
  ACTTTGGCACAGGTGCTGTGAAGATCACCCCCGCACATGACCAAAATGACTATGAAGTTGGGCAGCGGCA
  CGGGCTGGAGGCCATCAGCATCATGGACTCCCGGGGGGCCCTCATCAATGTGCCTCCGCCTTTCCTGGGC
  CTGCCCAGGTTTGAGGCCAGGAAAGCGGTGCTGGTGGCGCTGAAGGAGCGGGGACTGTTCCGTGGCATTG
  AGGACAACCCCATGGTGGTGCCACTTTGCAACCGGTCGAAGGACGTGGTAGAGCCTCTGCTGCGGCCGCA
  GTGGTACGTTCGCTGCGGGGAGATGGCCCAGGCTGCCAGCGCCGCTGTGACTCGGGGTGACCTCCGCATC
  CTGCCTGAGGCCCATCAGCGCACATGGCATGCCTGGATGGACAACATCCGGGAGTGGTGCATTTCCAGGC
  AGCTGTGGTGGGGCCATCGCATCCCAGCCTACTTTGTCACTGTCAGTGACCCAGCGGTGCCCCCTGGGGA
  GGACCCTGATGGGCGGTACTGGGTGAGTGGACGCAATGAGGCGGAGGCCCGGGAGAAGGCAGCCAAGGAG
  TTCGGAGTGTCCCCTGACAAGATCAGTCTCCAGCAAGATGAGGATGTATTGGATACCTGGTTCTCCTCTG
  GCCTCTTCCCCTTATCCATTTTGGGCTGGCCCAACCAGTCAGAAGACCTGAGTGTGTTCTACCCCGGGAC
  ACTGCTGGAGACCGGTCATGACATCCTCTTCTTCTGGGTGGCCCGGATGGTCATGCTGGGCCTGAAGCTC
  ACGGGCAGGCTGCCCTTTAGAGAGGTCTACCTCCATGCCATCGTGCGAGATGCTCACGGCCGGAAGATGA
  GCAAGTCTCTAGGCAATGTCATCGATCCCCTGGACGTCATCTATGGAATCTCCCTGCAGGGCCTCCACAA
  CCAGCTGCTGAACAGCAACCTGGATCCCAGCGAGGTGGAGAAGGCCAAAGAAGGGCAGAAAGCTGACTTC
  CCAGCGGGGATTCCTGAATGTGGCACCGATGCTCTCCGGTTTGGATTATGTGCCTACATGTCCCAGGGTC
  GTGACATCAACCTGGATGTGAACCGGATACTGGGTTACCGCCACTTCTGCAACAAGCTCTGGAATGCCAC
  CAAGTTTGCCCTTCGTGGCCTTGGGAAGGGTTTTGTGCCCTCACCCACCTCCCAGCCCGGAGGCCATGAG
  AGCCTGGTGGACCGCTGGATCCGCAGCCGCCTGACAGAGGCTGTGAGGCTCAGCAATCAAGGCTTCCAGG
  CCTACGACTTCCCGGCCGTCACCACTGCCCAGTACAGCTTCTGGCTCTATGAGCTCTGTGATGTCTACTT
  GGAGTGCCTGAAACCTGTACTGAATGGGGTGGACCAGGTGGCAGCTGAGTGTGCCCGCCAGACCCTGTAC
  ACTTGCCTGGACGTTGGCCTGCGGCTGCTCTCACCCTTCATGCCCTTCGTGACGGAGGAGCTGTTCCAGA
  GGCTGCCCCGGAGGATGCCGCAAGCTCCCCCTAGCCTCTGTGTTACCCCCTACCCGGAGCCCTCAGAGTG
  CTCCTGGAAGGACCCCGAGGCAGAAGCCGCCCTTGAGCTGGCGCTAAGCATCACGCGAGCCGTGCGCTCC
  CTGCGGGCCGACTACAACCTCACCCGGATCCGGCCTGACTGTTTCCTGGAAGTGGCGGATGAGGCCACGG
  GCGCCCTGGCATCGGCGGTGTCGGGCTACGTGCAGGCCCTGGCCAGCGCAGGTGTGGTGGCTGTTCTGGC
  CCTGGGGGCTCCCGCCCCCCAGGGTTGCGCTGTGGCTCTGGCTTCTGATCGCTGCTCCATCCACCTGCAG
  CTTCAGGGGCTGGTGGACCCTGCACGGGAGCTGGGCAAGCTGCAAGCCAAGCGAGTTGAGGCCCAGCGGC
  AGGCCCAGCGTCTGCGGGAACGCCGTGCTGCCTCGGGCTATCCTGTCAAGGTGCCGCTCGAAGTCCAGGA
  GGCAGATGAAGCCAAGCTCCAACAGACAGAAGCAGAGCTCAGGAAGGTGGATGAGGCCATCGCCCTATTC
  CAGAAGATGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201089 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSTLYVSPHPDAFPSLRALIAARYGEAGEGPGWGGAHPRICLQPPPTSRTSFPPPRLPALEQGPGGLWVW
  GATAVAQLLWPAGLGGPGGSRAAVLVQQWVSYADTELIPAACGATLPALGLRSSAQDPQAVLGALGRALS
  PLEEWLRLHTYLAGEAPTLADLAAVTALLLPFRYVLDPPARRIWNNVTRWFVTCVRQPEFRAVLGEVVLY
  SGARPLSHQPGPEAPALPKTAAQLKKEAKKREKLEKFQQKQKIQQQQPPPGEKKPKPEKREKRDPGVITY
  DLPTPPGEKKDVSGPMPDSYSPRYVEAAWYPWWEQQGFFKPEYGRPNVSAANPRGVFMMCIPPPNVTGSL
  HLGHALTNAIQDSLTRWHRMRGETTLWNPGCDHAGIATQVVVEKKLWREQGLSRHQLGREAFLQEVWKWK
  EEKGDRIYHQLKKLGSSLDWDRACFTMDPKLSAAVTEAFVRLHEEGIIYRSTRLVNWSCTLNSAISDIEV
  DKKELTGRTLLSVPGYKEKVEFGVLVSFAYKVQGSDSDEEVVVATTRIETMLGDVAVAVHPKDTRYQHLK
  GKNVIHPFLSRSLPIVFDEFVDMDFGTGAVKITPAHDQNDYEVGQRHGLEAISIMDSRGALINVPPPFLG
  LPRFEARKAVLVALKERGLFRGIEDNPMVVPLCNRSKDVVEPLLRPQWYVRCGEMAQAASAAVTRGDLRI
  LPEAHQRTWHAWMDNIREWCISRQLWWGHRIPAYFVTVSDPAVPPGEDPDGRYWVSGRNEAEAREKAAKE
  FGVSPDKISLQQDEDVLDTWFSSGLFPLSILGWPNQSEDLSVFYPGTLLETGHDILFFWVARMVMLGLKL
  TGRLPFREVYLHAIVRDAHGRKMSKSLGNVIDPLDVIYGISLQGLHNQLLNSNLDPSEVEKAKEGQKADF
  PAGIPECGTDALRFGLCAYMSQGRDINLDVNRILGYRHFCNKLWNATKFALRGLGKGFVPSPTSQPGGHE
  SLVDRWIRSRLTEAVRLSNQGFQAYDFPAVTTAQYSFWLYELCDVYLECLKPVLNGVDQVAAECARQTLY
  TCLDVGLRLLSPFMPFVTEELFQRLPRRMPQAPPSLCVTPYPEPSECSWKDPEAEAALELALSITRAVRS
  LRADYNLTRIRPDCFLEVADEATGALASAVSGYVQALASAGVVAVLALGAPAPQGCAVALASDRCSIHLQ
  LQGLVDPARELGKLQAKRVEAQRQAQRLRERRAASGYPVKVPLEVQEADEAKLQQTEAELRKVDEAIALF
  QKML
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006295
• ORF Size: 3792 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006295.1, NM_006295.2, NP_006286.1
• RefSeq Size: 4308 bp
• RefSeq ORF: 3795 bp
• Locus ID: 7407
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• MW: 140.5 kDa
• Gene Summary: 'Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]'