valyl tRNA synthetase (VARS) (NM_006295) Human Untagged Clone

CAT#: SC303765

VARS (untagged)-Human valyl-tRNA synthetase (VARS), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: VARS
• Synonyms: G7A; NDMSCA; VARS1; VARS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006295, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTCCACCCTCTACGTCTCCCCTCACCCAGATGCCTTCCCCAGCCTCCGAGCCCTCATAGCCGCTCGCT
  ATGGGGAGGCTGGGGAGGGTCCCGGATGGGGAGGAGCCCACCCCCGCATCTGTCTCCAGCCACCCCCGAC
  TAGCAGGACTCCCTTTCCCCCACCCCGCCTGCCGGCCCTGGAGCAGGGGCCCGGTGGGCTCTGGGTGTGG
  GGGGCCACGGCTGTGGCCCAGCTGCTGTGGCCAGCAGGCCTGGGGGGCCCAGGGGGCAGCCGGGCGGCTG
  TCCTTGTCCAACAGTGGGTCAGTTACGCCGACACGGAGTTAATACCAGCTGCCTGTGGAGCAACGCTGCC
  GGCCCTGGGACTCCGAAGCTCGGCCCAGGACCCCCAGGCTGTGCTGGGGGCCCTGGGCAGGGCCCTGAGC
  CCCTTGGAGGAGTGGCTTCGGCTGCACACCTACTTGGCCGGGGAGGCCCCCACTCTGGCTGACCTGGCGG
  CTGTCACAGCCTTGCTGCTGCCTTTCCGATACGTCCTAGACCCACCTGCCCGCCGGATCTGGAATAATGT
  GACTCGCTGGTTTGTCACGTGTGTCCGGCAGCCAGAATTCCGAGCCGTGCTAGGAGAAGTGGTTCTATAC
  TCAGGAGCCAGGCCTCTCTCTCATCAGCCAGGCCCCGAGGCTCCTGCCCTCCCAAAGACAGCTGCTCAGC
  TCAAGAAAGAGGCAAAGAAACGGGAGAAGCTAGAGAAATTCCAACAGAAGCAGAAGATCCAACAGCAGCA
  GCCACCTCCAGGGGAGAAGAAACCAAAACCAGAGAAGAGGGAGAAACGGGATCCTGGGGTCATTACCTAT
  GACCTCCCAACCCCACCCGGGGAAAAGAAAGATGTCAGTGGCCCCATGCCCGACTCCTACAGCCCTCGGT
  ATGTGGAGGCTGCCTGGTACCCTTGGTGGGAGCAGCAGGGCTTCTTCAAGCCAGAGTATGGGCGTCCTAA
  TGTGTCAGCAGCAAATCCCCGAGGTGTCTTCATGATGTGCATCCCACCCCCCAATGTGACAGGCTCCCTG
  CACCTGGGCCATGCACTCACCAACGCCATCCAGGACTCCCTGACTCGATGGCACCGCATGCGTGGGGAGA
  CCACCCTGTGGAACCCTGGCTGTGACCATGCAGGTATTGCCACCCAGGTGGTGGTGGAGAAGAAGCTATG
  GCGTGAGCAGGGACTGAGCCGGCACCAGCTGGGCCGCGAGGCCTTTCTACAGGAAGTCTGGAAGTGGAAG
  GAGGAGAAAGGTGACCGGATTTACCACCAGTTGAAGAAGCTTGGCAGCTCCTTGGACTGGGATCGAGCCT
  GTTTCACCATGGACCCTAAACTCTCAGCAGCTGTGACAGAGGCCTTTGTCCGGCTTCACGAGGAAGGCAT
  CATCTATCGCAGTACCCGCCTTGTTAACTGGTCCTGCACCCTCAACTCCGCCATCTCTGACATTGAGGTG
  GATAAGAAGGAGCTGACAGGTCGCACCCTGCTCTCCGTGCCTGGCTACAAGGAGAAGGTGGAGTTCGGGG
  TCCTCGTGTCCTTTGCCTATAAGGTCCAAGGCTCAGATAGCGACGAGGAGGTGGTGGTGGCAACAACTCG
  GATCGAGACAATGCTGGGAGATGTGGCTGTAGCTGTGCACCCCAAAGATACCAGATACCAGCACCTGAAG
  GGGAAGAACGTGATCCACCCATTCCTGTCTCGGAGCCTTCCCATTGTCTTCGATGAATTTGTGGACATGG
  ACTTTGGCACAGGTGCTGTGAAGATCACCCCCGCACATGACCAAAATGACTATGAAGTTGGGCAGCGGCA
  CGGGCTGGAGGCCATCAGCATCATGGACTCCCGGGGGGCCCTCATCAATGTGCCTCCGCCTTTCCTGGGC
  CTGCCCAGGTTTGAGGCCAGGAAAGCGGTGCTGGTGGCGCTGAAGGAGCGGGGACTGTTCCGTGGCATTG
  AGGACAACCCCATGGTGGTGCCACTTTGCAACCGGTCGAAGGACGTGGTAGAGCCTCTGCTGCGGCCGCA
  GTGGTACGTTCGCTGCGGGGAGATGGCCCAGGCTGCCAGCGCCGCTGTGACTCGGGGTGACCTCCGCATC
  CTGCCTGAGGCCCATCAGCGCACATGGCATGCCTGGATGGACAACATCCGGGAGTGGTGCATTTCCAGGC
  AGCTGTGGTGGGGCCATCGCATCCCAGCCTACTTTGTCACTGTCAGTGACCCAGCGGTGCCCCCTGGGGA
  GGACCCTGATGGGCGGTACTGGGTGAGTGGACGCAATGAGGCGGAGGCCCGGGAGAAGGCAGCCAAGGAG
  TTCGGAGTGTCCCCTGACAAGATCAGTCTCCAGCAAGATGAGGATGTATTGGATACCTGGTTCTCCTCTG
  GCCTCTTCCCCTTATCCATTTTGGGCTGGCCCAACCAGTCAGAAGACCTGAGTGTGTTCTACCCCGGGAC
  ACTGCTGGAGACCGGTCATGACATCCTCTTCTTCTGGGTGGCCCGGATGGTCATGCTGGGCCTGAAGCTC
  ACGGGCAGGCTGCCCTTTAGAGAGGTCTACCTCCATGCCATCGTGCGAGATGCTCACGGCCGGAAGATGA
  GCAAGTCTCTAGGCAATGTCATCGATCCCCTGGACGTCATCTATGGAATCTCCCTGCAGGGCCTCCACAA
  CCAGCTGCTGAACAGCAACCTGGATCCCAGCGAGGTGGAGAAGGCCAAAGAAGGGCAGAAAGCTGACTTC
  CCAGCGGGGATTCCTGAATGTGGCACCGATGCTCTCCGGTTTGGATTATGTGCCTACATGTCCCAGGGTC
  GTGACATCAACCTGGATGTGAACCGGATACTGGGTTACCGCCACTTCTGCAACAAGCTCTGGAATGCCAC
  CAAGTTTGCCCTTCGTGGCCTTGGGAAGGGTTTTGTGCCCTCACCCACCTCCCAGCCCGGAGGCCATGAG
  AGCCTGGTGGACCGCTGGATCCGCAGCCGCCTGACAGAGGCTGTGAGGCTCAGCAATCAAGGCTTCCAGG
  CCTACGACTTCCCGGCCGTCACCACTGCCCAGTACAGCTTCTGGCTCTATGAGCTCTGTGATGTCTACTT
  GGAGTGCCTGAAACCTGTACTGAATGGGGTGGACCAGGTGGCAGCTGAGTGTGCCCGCCAGACCCTGTAC
  ACTTGCCTGGACGTTGGCCTGCGGCTGCTCTCACCCTTCATGCCCTTCGTGACGGAGGAGCTGTTCCAGA
  GGCTGCCCCGGAGGATGCCGCAAGCTCCCCCTAGCCTCTGTGTTACCCCCTACCCGGAGCCCTCAGAGTG
  CTCCTGGAAGGACCCCGAGGCAGAAGCCGCCCTTGAGCTGGCGCTAAGCATCACGCGAGCCGTGCGCTCC
  CTGCGGGCCGACTACAACCTCACCCGGATCCGGCCTGACTGTTTCCTGGAAGTGGCGGATGAGGCCACGG
  GCGCCCTGGCATCGGCGGTGTCGGGCTACGTGCAGGCCCTGGCCAGCGCAGGTGTGGTGGCTGTTCTGGC
  CCTGGGGGCTCCCGCCCCCCAGGGTTGCGCTGTGGCTCTGGCTTCTGATCGCTGCTCCATCCACCTGCAG
  CTTCAGGGGCTGGTGGACCCTGCACGGGAGCTGGGCAAGCTGCAAGCCAAGCGAGTTGAGGCCCAGCGGC
  AGGCCCAGCGTCTGCGGGAACGCCGTGCTGCCTCGGGCTATCCTGTCAAGGTGCCGCTCGAAGTCCAGGA
  GGCAGATGAAGCCAAGCTCCAACAGACAGAAGCAGAGCTCAGGAAGGTGGATGAGGCCATCGCCCTATTC
  CAGAAGATGCTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006295
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006295.2, NP_006286.1
• RefSeq Size: 4308 bp
• RefSeq ORF: 3795 bp
• Locus ID: 7407
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Valine, leucine and isoleucine biosynthesis
• Gene Summary: 'Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. The protein encoded by this gene belongs to class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]'