PLAT (NM_033011) Human Tagged ORF Clone
CAT#: RC201146L3
- LentiORF®
Lenti ORF clone of Human plasminogen activator, tissue (PLAT), transcript variant 3, Myc-DDK-tagged
"NM_033011" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PLAT |
Synonyms | T-PA; TPA |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC201146).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_033011 |
ORF Size | 1548 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_033011.1, NP_127509.1 |
RefSeq Size | 3035 bp |
RefSeq ORF | 1551 bp |
Locus ID | 5327 |
Cytogenetics | 8p11.21 |
Domains | KR, Tryp_SPc, EGF |
Protein Families | Druggable Genome, Protease, Secreted Protein |
Protein Pathways | Complement and coagulation cascades |
MW | 57.4 kDa |
Gene Summary | 'This gene encodes tissue-type plasminogen activator, a secreted serine protease that converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. The encoded preproprotein is proteolytically processed by plasmin or trypsin to generate heavy and light chains. These chains associate via disulfide linkages to form the heterodimeric enzyme. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, while decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC109578 | PLAT (untagged)-Human plasminogen activator, tissue (PLAT), transcript variant 3 |
USD 760.00 |
|
RC201146 | PLAT (Myc-DDK-tagged)-Human plasminogen activator, tissue (PLAT), transcript variant 3 |
USD 420.00 |
|
RG201146 | PLAT (GFP-tagged) - Human plasminogen activator, tissue (PLAT), transcript variant 3 |
USD 460.00 |
|
RC201146L1 | Lenti ORF clone of Human plasminogen activator, tissue (PLAT), transcript variant 3, Myc-DDK-tagged |
USD 768.00 |
|
RC201146L2 | Lenti ORF clone of Human plasminogen activator, tissue (PLAT), transcript variant 3, mGFP tagged |
USD 620.00 |
|
RC201146L4 | Lenti ORF clone of Human plasminogen activator, tissue (PLAT), transcript variant 3, mGFP tagged |
USD 620.00 |
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