PCDHGC3 (NM_002588) Human Tagged ORF Clone

CAT#: RC201287

PCDHGC3 (Myc-DDK-tagged)-Human protocadherin gamma subfamily C, 3 (PCDHGC3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY419228) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201287 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCDHGC3
• Synonyms: PC43; PCDH-GAMMA-C3; PCDH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201287 representing NM_002588
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCCCAGAGGCCTGGAGGAGCGGACTGGTAAGCACCGGGAGGGTAGTGGGAGTTTTGCTTCTGCTTG
  GTGCCTTGAACAAGGCTTCCACGGTCATTCACTATGAGATCCCGGAGGAAAGAGAGAAGGGTTTCGCTGT
  GGGCAACGTGGTCGCGAACCTTGGTTTGGATCTCGGTAGCCTCTCAGCCCGCAGGTTCCGGGTGGTGTCT
  GGAGCTAGCCGAAGATTCTTTGAGGTGAACCGGGAGACCGGAGAGATGTTTGTGAACGACCGTCTGGATC
  GAGAGGAGCTGTGTGGGACACTGCCCTCTTGCACTGTAACTCTGGAGTTGGTAGTGGAGAACCCGCTGGA
  GCTGTTCAGCGTGGAAGTGGTGATCCAGGACATCAACGACAACAATCCTGCTTTCCCTACCCAGGAAATG
  AAATTGGAGATTAGCGAGGCCGTGGCTCCGGGGACGCGCTTTCCGCTCGAGAGCGCGCACGATCCCGATG
  TGGGAAGCAACTCTTTACAAACCTATGAGCTGAGCCGAAATGAATACTTTGCGCTTCGCGTGCAGACGCG
  GGAGGACAGCACCAAGTACGCGGAGCTGGTGTTGGAGCGCGCCCTGGACCGAGAACGGGAGCCTAGTCTC
  CAGTTAGTGCTGACGGCGTTGGACGGAGGGACCCCAGCTCTCTCCGCCAGCCTGCCTATTCACATCAAGG
  TGCTGGACGCGAATGACAATGCGCCTGTCTTCAACCAGTCCTTGTACCGGGCGCGCGTCCTGGAGGATGC
  ACCCTCCGGCACGCGCGTGGTACAAGTCCTTGCAACGGATCTGGATGAAGGCCCCAACGGTGAAATTATT
  TACTCCTTCGGCAGCCACAACCGCGCCGGCGTGCGGCAACTATTCGCCTTAGACCTTGTAACCGGGATGC
  TGACAATCAAGGGTCGGCTGGACTTCGAGGACACCAAACTCCATGAGATTTACATCCAGGCCAAAGACAA
  GGGCGCCAATCCCGAAGGAGCACATTGCAAAGTGTTGGTGGAGGTTGTGGATGTGAATGACAACGCCCCG
  GAGATCACAGTCACCTCCGTGTACAGCCCAGTACCCGAGGATGCCCCTCTGGGGACTGTCATCGCTTTGC
  TCAGTGTGACTGACCTGGATGCTGGCGAGAACGGGCTGGTGACCTGCGAAGTTCCACCGGGTCTCCCTTT
  CAGCCTTACTTCTTCCCTCAAGAATTACTTCACTTTGAAAACCAGTGCAGACCTGGATCGGGAGACTGTG
  CCAGAATACAACCTCAGCATCACCGCCCGAGACGCCGGAACCCCTTCCCTCTCAGCCCTTACAATAGTGC
  GTGTTCAAGTGTCCGACATCAATGACAACCCTCCACAATCTTCTCAATCTTCCTACGACGTTTACATTGA
  AGAAAACAACCTCCCCGGGGCTCCAATACTAAACCTAAGTGTCTGGGACCCCGACGCCCCGCAGAATGCT
  CGGCTTTCTTTCTTTCTCTTGGAGCAAGGAGCTGAAACCGGGCTAGTGGGTCGCTATTTCACAATAAATC
  GTGACAATGGCATAGTGTCATCCTTAGTGCCCCTAGACTATGAGGATCGGCGGGAATTTGAATTAACAGC
  TCATATCAGCGATGGGGGCACCCCGGTCCTAGCCACCAACATCAGCGTGAACATATTTGTCACTGATCGC
  AATGACAATGCCCCCCAGGTCCTATATCCTCGGCCAGGTGGGAGCTCGGTGGAGATGCTGCCTCGAGGTA
  CCTCAGCTGGCCACCTAGTGTCACGGGTGGTAGGCTGGGACGCGGATGCAGGGCACAATGCCTGGCTCTC
  CTACAGTCTCTTGGGATCCCCTAACCAGAGCCTTTTTGCCATAGGGCTGCACACTGGTCAAATCAGTACT
  GCCCGTCCAGTCCAAGACACAGATTCACCCAGGCAGACTCTCACGGTCTTGATCAAAGACAATGGGGAGC
  CTTCGCTCTCCACCACTGCTACCCTCACTGTGTCAGTAACCGAGGACTCTCCTGAAGCCCGAGCCGAGTT
  CCCCTCTGGCTCTGCCCCCCGGGAGCAGAAAAAAAATCTCACCTTTTATCTACTTCTTTCTCTAATCCTG
  GTTTCTGTGGGGTTTGTGGTCACAGTGTTCGGAGTAATCATATTCAAAGTTTACAAGTGGAAGCAGTCTA
  GAGACCTATACCGAGCCCCGGTGAGCTCACTGTACCGAACACCAGGGCCCTCCTTGCACGCGGACGCCGT
  GCGGGGAGGCCTGATGTCGCCGCACCTTTACCATCAGGTGTATCTCACCACGGACTCCCGCCGCAGCGAC
  CCGCTGCTGAAGAAACCTGGTGCAGCCAGTCCACTGGCCAGCCGCCAGAACACGCTGCGGAGCTGTGATC
  CGGTGTTCTATAGGCAGGTGTTGGGTGCAGAGAGCGCCCCTCCCGGACAGCAAGCCCCGCCCAACACGGA
  CTGGCGTTTCTCTCAGGCCCAGAGACCCGGCACCAGCGGCTCCCAAAATGGCGATGACACCGGCACCTGG
  CCCAACAACCAGTTTGACACAGAGATGCTGCAAGCCATGATCTTGGCGTCCGCCAGTGAAGCTGCTGATG
  GGAGCTCCACCCTGGGAGGGGGTGCCGGCACCATGGGATTGAGCGCCCGCTACGGACCCCAGTTCACCCT
  GCAGCACGTGCCCGACTACCGCCAGAATGTCTACATCCCAGGCAGCAATGCCACACTGACCAACGCAGCT
  GGCAAGCGGGATGGCAAGGCCCCAGCAGGTGGCAATGGCAACAAGAAGAAGTCGGGCAAGAAGGAGAAGA
  AG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201287 representing NM_002588
  Red=Cloning site Green=Tags(s)
  
  MVPEAWRSGLVSTGRVVGVLLLLGALNKASTVIHYEIPEEREKGFAVGNVVANLGLDLGSLSARRFRVVS
  GASRRFFEVNRETGEMFVNDRLDREELCGTLPSCTVTLELVVENPLELFSVEVVIQDINDNNPAFPTQEM
  KLEISEAVAPGTRFPLESAHDPDVGSNSLQTYELSRNEYFALRVQTREDSTKYAELVLERALDREREPSL
  QLVLTALDGGTPALSASLPIHIKVLDANDNAPVFNQSLYRARVLEDAPSGTRVVQVLATDLDEGPNGEII
  YSFGSHNRAGVRQLFALDLVTGMLTIKGRLDFEDTKLHEIYIQAKDKGANPEGAHCKVLVEVVDVNDNAP
  EITVTSVYSPVPEDAPLGTVIALLSVTDLDAGENGLVTCEVPPGLPFSLTSSLKNYFTLKTSADLDRETV
  PEYNLSITARDAGTPSLSALTIVRVQVSDINDNPPQSSQSSYDVYIEENNLPGAPILNLSVWDPDAPQNA
  RLSFFLLEQGAETGLVGRYFTINRDNGIVSSLVPLDYEDRREFELTAHISDGGTPVLATNISVNIFVTDR
  NDNAPQVLYPRPGGSSVEMLPRGTSAGHLVSRVVGWDADAGHNAWLSYSLLGSPNQSLFAIGLHTGQIST
  ARPVQDTDSPRQTLTVLIKDNGEPSLSTTATLTVSVTEDSPEARAEFPSGSAPREQKKNLTFYLLLSLIL
  VSVGFVVTVFGVIIFKVYKWKQSRDLYRAPVSSLYRTPGPSLHADAVRGGLMSPHLYHQVYLTTDSRRSD
  PLLKKPGAASPLASRQNTLRSCDPVFYRQVLGAESAPPGQQAPPNTDWRFSQAQRPGTSGSQNGDDTGTW
  PNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAA
  GKRDGKAPAGGNGNKKKSGKKEKK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002588
• ORF Size: 2802 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002588.2, NM_002588.3, NP_002579.2
• RefSeq Size: 4726 bp
• RefSeq ORF: 2805 bp
• Locus ID: 5098
• Cytogenetics: 5q31.3
• Domains: CA
• Protein Families: Transmembrane
• MW: 101.08 kDa
• Gene Summary: 'This gene is a member of the protocadherin gamma gene cluster, one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes. [provided by RefSeq, Jul 2008]'