ZNF420 (NM_144689) Human Tagged ORF Clone

CAT#: RC201401

ZNF420 (Myc-DDK-tagged)-Human zinc finger protein 420 (ZNF420)

Product Images

Western blot validation of overexpression lysate (Cat# LY408182) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201401 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF420
• Synonyms: APAK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201401 representing NM_144689
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTCGGAAATTAGTGATGTTCAGGGATGTTGCCATTGACTTCTCTCAGGAAGAGTGGGAATGCCTGG
  ACTCTGCTCAGAGAGATTTGTATAGAGATGTGATGTTGGAGAACTATAGCAACTTGGTATCACTAGACTT
  GCCTTCAAGGTGTGCAAGTAAGGACTTATCTCCAGAAAAGAACACTTATGAAACAGAATTATCCCAATGG
  GAAATGAGTGACAGACTTGAAAACTGTGATCTTGAAGAGTCCAATTCCAGGGATTATTTGGAAGCCAAAG
  GCAAGATGGAGAAGCAACAAGAAAATCAGAAGGAATATTTCAGGCAAGGGATGATCATATATGACAAAAT
  GTCCATTTTCAACCAGCATACTTACTTATCTCAACATTCAAGATGTCATTCTACTGAGAAACCCTATAAA
  TGTAAGGAATGTGGGAAAGCCTTCAGACGAGCCTCACACCTAACACAACATCAAAGTATTCATACTGGTG
  AAAAACCCTATGAATGTAAGCAATGCGGGAAGGCCTTTAGTCGTGATTCACAACTCAGTCTTCATCAGAG
  ACTTCATACTGGTGAGAAACCCTATGCATGTAAGGAATGTGGGAAGGCCTTTACTCAAAGCTCACAACTT
  ATTTTACATCATAGAATTCATACTGGTGAAAAACCATATAAATGTGAAGAATGTGGGAAAGCCTTTATTC
  GTAGCTCACAACTTACCCGACATCAAAAAGTTCATACTGGTGAGAAACCTTATGAATGTAAAGAATGTGG
  GAAGGCCTTTACTCAGAATTCACAACTTACACTACACCAGAGACTTCATACTGGTGAAAAGCTCTATGAA
  TGTAAAGAATGTAGGAAGGTCTTTACTCAGCTCTCACAACTTATTCTGCATAAGAGAATTCATACCGGTG
  AGAAACCCTATGAATGTAAGGAATGTGGAAAAGCTTTTATTTGTGGCTCACAGCTTTCTCAACATCAGAA
  AATTCATAATGGGGAAAAACCATATGAATGTAAGGAATGTGGAAGGGCCTTTATTCGGGGCTCACTACTG
  ATGCAACATCAGAGGATTCATACTGGTGAAAAACCCTATAAATGTGAAGAATGTGGGAAGGCCTTTATCC
  GTGGCTCACAACTTACTCAACACCAGAGAATTCACACCAATGAAAAGCCCTATGAATGTAAGGAATGTGG
  AAAGATGTTTAGTCATGGCTCACAACTTACTCAACATCAGAGAATACACACTGGTGAGAAACCCTATCAA
  TGTAAGGAATGTGGAAAAGCGTTTAATCGTGGCTCACTCCTTACACGACACCAGAGGATTCATACTGGTG
  AGAAACCCTATGAATGTAAAGAATGTGGAAAAACCTTTAGTCGTGGCTCAGAACTTACTCAACATGAGCG
  AATTCACACAGGTGAGAAACCCTATGAATGTAAGGAATGTGGGAAATCTTTTATTCGTGGTTCCCAGCTT
  ACTCAACATCAGAGAATCCATACTGGTGAGAAACCTTATGAATGTAAAGAATGTAGAATGGCCTTTACTC
  AGAGTTCACATCTTTCCCAACATCAAAGACTTCACACTGGTGAGAAACCCTATGTGTGTAATGAATGTGG
  AAAGGCCTTTGCGCGTGGCTTACTACTTATACAACATCAGAGAATTCATACTGGTGAGAAACCATATCAA
  TGTAAGGAATGTGGGAAAGCCTTTATTCGTGGTTCACAGTTGACTCAACATCAGCGAATTCACACTGGAG
  AAAAACCCTATGAATGCAAGGAGTGTGGCAAGGCCTTTAGTCATGGCTCTCAGCTTACTCTACATCAGAG
  AATCCATACTGGTGAGAAGCCCTATGAATGCAGAGAATGTAGAAAGGCCTTTACTCAGAGTTCACATCTT
  TCTCGGCATCAGAGAATTCATACTGGTGAGAAACCATATCAATGTAAGGAATGTGGGAAGGCCTTTACTC
  GTGGTTCACAGCTAACTCAACATCAGAGAATTCATATCAGTGAGAAATCTTTTGAATATAAGGAATGTGG
  GATTGACTTTAGTCATGGCTCACAAGTTTACATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201401 representing NM_144689
  Red=Cloning site Green=Tags(s)
  
  MARKLVMFRDVAIDFSQEEWECLDSAQRDLYRDVMLENYSNLVSLDLPSRCASKDLSPEKNTYETELSQW
  EMSDRLENCDLEESNSRDYLEAKGKMEKQQENQKEYFRQGMIIYDKMSIFNQHTYLSQHSRCHSTEKPYK
  CKECGKAFRRASHLTQHQSIHTGEKPYECKQCGKAFSRDSQLSLHQRLHTGEKPYACKECGKAFTQSSQL
  ILHHRIHTGEKPYKCEECGKAFIRSSQLTRHQKVHTGEKPYECKECGKAFTQNSQLTLHQRLHTGEKLYE
  CKECRKVFTQLSQLILHKRIHTGEKPYECKECGKAFICGSQLSQHQKIHNGEKPYECKECGRAFIRGSLL
  MQHQRIHTGEKPYKCEECGKAFIRGSQLTQHQRIHTNEKPYECKECGKMFSHGSQLTQHQRIHTGEKPYQ
  CKECGKAFNRGSLLTRHQRIHTGEKPYECKECGKTFSRGSELTQHERIHTGEKPYECKECGKSFIRGSQL
  TQHQRIHTGEKPYECKECRMAFTQSSHLSQHQRLHTGEKPYVCNECGKAFARGLLLIQHQRIHTGEKPYQ
  CKECGKAFIRGSQLTQHQRIHTGEKPYECKECGKAFSHGSQLTLHQRIHTGEKPYECRECRKAFTQSSHL
  SRHQRIHTGEKPYQCKECGKAFTRGSQLTQHQRIHISEKSFEYKECGIDFSHGSQVYM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144689
• ORF Size: 2064 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_144689.2, NM_144689.3, NM_144689.4, NP_653290.2
• RefSeq Size: 2990
• RefSeq ORF: 2067
• Locus ID: 147923
• Domains: KRAB, zf-C2H2
• Protein Families: Transcription Factors
• MW: 80.1 kDa
• Gene Summary: The protein encoded by this gene is a KRAB-type zinc finger protein that negatively-regulates p53-mediated apoptosis. Under stress conditions, the encoded protein is phosphorylated by ATM and dissociates from p53, which activates p53 and initiates apoptosis. [provided by RefSeq, Jul 2016]