DR5 (TNFRSF10B) (NM_003842) Human Tagged ORF Clone

CAT#: RC201588

TNFRSF10B (Myc-DDK-tagged)-Human tumor necrosis factor receptor superfamily, member 10b (TNFRSF10B), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401260) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201588 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TNFRSF10B
• Synonyms: CD262; DR5; KILLER; KILLER/DR5; TRAIL-R2; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; ZTNFR9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201588 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAACAACGGGGACAGAACGCCCCGGCCGCTTCGGGGGCCCGGAAAAGGCACGGCCCAGGACCCAGGG
  AGGCGCGGGGAGCCAGGCCTGGGCTCCGGGTCCCCAAGACCCTTGTGCTCGTTGTCGCCGCGGTCCTGCT
  GTTGGTCTCAGCTGAGTCTGCTCTGATCACCCAACAAGACCTAGCTCCCCAGCAGAGAGCGGCCCCACAA
  CAAAAGAGGTCCAGCCCCTCAGAGGGATTGTGTCCACCTGGACACCATATCTCAGAAGACGGTAGAGATT
  GCATCTCCTGCAAATATGGACAGGACTATAGCACTCACTGGAATGACCTCCTTTTCTGCTTGCGCTGCAC
  CAGGTGTGATTCAGGTGAAGTGGAGCTAAGTCCCTGCACCACGACCAGAAACACAGTGTGTCAGTGCGAA
  GAAGGCACCTTCCGGGAAGAAGATTCTCCTGAGATGTGCCGGAAGTGCCGCACAGGGTGTCCCAGAGGGA
  TGGTCAAGGTCGGTGATTGTACACCCTGGAGTGACATCGAATGTGTCCACAAAGAATCAGGTACAAAGCA
  CAGTGGGGAAGCCCCAGCTGTGGAGGAGACGGTGACCTCCAGCCCAGGGACTCCTGCCTCTCCCTGTTCT
  CTCTCAGGCATCATCATAGGAGTCACAGTTGCAGCCGTAGTCTTGATTGTGGCTGTGTTTGTTTGCAAGT
  CTTTACTGTGGAAGAAAGTCCTTCCTTACCTGAAAGGCATCTGCTCAGGTGGTGGTGGGGACCCTGAGCG
  TGTGGACAGAAGCTCACAACGACCTGGGGCTGAGGACAATGTCCTCAATGAGATCGTGAGTATCTTGCAG
  CCCACCCAGGTCCCTGAGCAGGAAATGGAAGTCCAGGAGCCAGCAGAGCCAACAGGTGTCAACATGTTGT
  CCCCCGGGGAGTCAGAGCATCTGCTGGAACCGGCAGAAGCTGAAAGGTCTCAGAGGAGGAGGCTGCTGGT
  TCCAGCAAATGAAGGTGATCCCACTGAGACTCTGAGACAGTGCTTCGATGACTTTGCAGACTTGGTGCCC
  TTTGACTCCTGGGAGCCGCTCATGAGGAAGTTGGGCCTCATGGACAATGAGATAAAGGTGGCTAAAGCTG
  AGGCAGCGGGCCACAGGGACACCTTGTACACGATGCTGATAAAGTGGGTCAACAAAACCGGGCGAGATGC
  CTCTGTCCACACCCTGCTGGATGCCTTGGAGACGCTGGGAGAGAGACTTGCCAAGCAGAAGATTGAGGAC
  CACTTGTTGAGCTCTGGAAAGTTCATGTATCTAGAAGGTAATGCAGACTCTGCCATGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201588 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEQRGQNAPAASGARKRHGPGPREARGARPGLRVPKTLVLVVAAVLLLVSAESALITQQDLAPQQRAAPQ
  QKRSSPSEGLCPPGHHISEDGRDCISCKYGQDYSTHWNDLLFCLRCTRCDSGEVELSPCTTTRNTVCQCE
  EGTFREEDSPEMCRKCRTGCPRGMVKVGDCTPWSDIECVHKESGTKHSGEAPAVEETVTSSPGTPASPCS
  LSGIIIGVTVAAVVLIVAVFVCKSLLWKKVLPYLKGICSGGGGDPERVDRSSQRPGAEDNVLNEIVSILQ
  PTQVPEQEMEVQEPAEPTGVNMLSPGESEHLLEPAEAERSQRRRLLVPANEGDPTETLRQCFDDFADLVP
  FDSWEPLMRKLGLMDNEIKVAKAEAAGHRDTLYTMLIKWVNKTGRDASVHTLLDALETLGERLAKQKIED
  HLLSSGKFMYLEGNADSAMS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003842
• ORF Size: 1320 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003842.2, NM_003842.3, NM_003842.4, NP_003833.3
• RefSeq Size: 4154 bp
• RefSeq ORF: 1323 bp
• Locus ID: 8795
• Domains: DEATH, TNFR
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Apoptosis, Cytokine-cytokine receptor interaction, Natural killer cell mediated cytotoxicity, p53 signaling pathway
• MW: 47.9 kDa
• Gene Summary: The protein encoded by this gene is a member of the TNF-receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. Two transcript variants encoding different isoforms and one non-coding transcript have been found for this gene. [provided by RefSeq, Mar 2009]