BAF53A (ACTL6A) (NM_177989) Human Tagged ORF Clone

CAT#: RC201689

ACTL6A (Myc-DDK-tagged)-Human actin-like 6A (ACTL6A), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406030) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205341 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACTL6A
• Synonyms: ACTL6; Arp4; ARPN-BETA; BAF53A; INO80K
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC201689 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAAAGGCAAACAAGGCGGTC
  CCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGAGAATATGGAGGCCATTTCACCTCTAAA
  AAATGGGATGGTTGAAGACTGGGATAGTTTCCAAGCTATTTTGGATCATACCTACAAAATGCATGTCAAA
  TCAGAAGCCAGTCTCCATCCTGTTCTCATGTCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAAC
  TGACAGAGTTAATGTTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGC
  ATTTGCTAATGGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAATTCCAGTC
  CACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGACTTTATTACTATGCAGT
  GCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTCCTCCATATATGATTGCATCAAAAGAAGCTGT
  TCGTGAAGGATCTCCAGCAAACTGGAAAAGAAAAGAGAAGTTGCCTCAGGTTACGAGGTCTTGGCACAAT
  TATATGTGTAATTGTGTTATCCAGGATTTTCAAGCTTCGGTACTTCAAGTGTCAGATTCAACTTATGATG
  AACAAGTGGCTGCACAGATGCCAACTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTGC
  AGAGCGGCTAAAGATTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAGGAAACACAATG
  TTAGGAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTGATATCAGACCAGGTCTCTATG
  GCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGAGTTTTACTGACAGGTTGAATAGAGAGCTGTC
  TCAGAAAACTCCTCCAAGTATGCGGTTGAAATTGATTGCAAATAATACAACAGTGGAACGGAGGTTTAGC
  TCATGGATTGGCGGCTCCATTCTAGCCTCTTTGGGTACCTTTCAACAGATGTGGATTTCCAAGCAAGAAT
  ATGAAGAAGGAGGGAAGCAGTGTGTAGAAAGAAAATGCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC201689 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVVERDDGSTLMEIDGDKGKQGGPTYYIDTNALRVPRENMEAISPLKNGMVEDWDSFQAILDHTYKMHVK
  SEASLHPVLMSEAPWNTRAKREKLTELMFEHYNIPAFFLCKTAVLTAFANGRSTGLILDSGATHTTAIPV
  HDGYVLQQGIVKSPLAGDFITMQCRELFQEMNIELVPPYMIASKEAVREGSPANWKRKEKLPQVTRSWHN
  YMCNCVIQDFQASVLQVSDSTYDEQVAAQMPTVHYEFPNGYNCDFGAERLKIPEGLFDPSNVKGLSGNTM
  LGVSHVVTTSVGMCDIDIRPGLYGSVIVAGGNTLIQSFTDRLNRELSQKTPPSMRLKLIANNTTVERRFS
  SWIGGSILASLGTFQQMWISKQEYEEGGKQCVERKCP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177989
• ORF Size: 1161 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177989.1, NM_177989.2, NM_177989.3, NP_817126.1
• RefSeq Size: 1764 bp
• RefSeq ORF: 1164 bp
• Locus ID: 86
• Cytogenetics: 3q26.33
• Protein Families: Druggable Genome, Transcription Factors
• MW: 43.2 kDa
• Gene Summary: 'This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]'