BAF53A (ACTL6A) (NM_177989) Human Untagged Clone

CAT#: SC108893

ACTL6A (untagged)-Human actin-like 6A (ACTL6A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACTL6A
• Synonyms: ACTL6; Arp4; ARPN-BETA; BAF53A; INO80K
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_177989 edited
  GAATTCGGCACGAGGAGTCACTTCGCCAGTTAGCCCTTAGGGTAGGAGTCGCGCCGGCAG
  CAGCCATGAGCGGCGGCGTGTACGGGGGAGATGAAGTTGGAGCCCTTGTTTTTGACATTG
  GATCCTATACTGTGAGAGCTGGTTATGCTGGTGAGGACTGCCCCAAGGTGGATTTTCCTA
  CAGCTATTGGTATGGTGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCG
  ATAAAGGCAAACAAGGCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGA
  GGGAGAATATGGAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTT
  TCCAAGCTATTTTGGATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATC
  CTGTTCTCATGTCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGT
  TAATGTTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAG
  CATTTGCTAATGGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTG
  CAATTCCAGTCCACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTG
  GAGACTTTATTACTATGCAGTGCAGAGAACTCTTCCAAGAAATGAATATTGAATTGGTTC
  CTCCATATATGATTGCAACAAAAGAAGCTGTTCGTGAAGGATCTCCAGCAAACTGGAAAA
  GAAAAGAGAAGTTGCCTCAGGTTACGAGGTCTTGGCACAATTATATGTGTAATTGTGTTA
  TCCAGGATTTTCAAGCTTCGGTACTTCAAGTGTCAGATTCAACTTATGATGAACAAGTGG
  CTGCACAGATGCCAACTGTTCATTATGAATTCCCCAATGGCTACAATTGTGATTTTGGTG
  CAGAGCGGCTAAAGATTCCAGAAGGATTATTTGACCCTTCCAATGTAAAGGGGTTATCAG
  GAAACACAATGTTAGGAGTCAGTCATGTTGTCACCACAAGTGTTGGGATGTGTGATATTG
  ACATCAGACCAGGTCTCTATGGCAGTGTAATAGTGGCAGGAGGAAACACACTAATACAGA
  GTTTTACTGACAGGTTGAATAGAGAGCTGTCTCAGAAAACTCCTCCAAGTATGCGGTTGA
  AATTGATTGCAAATAATACAACAGTGGAACGGAGGTTTAGCTCATGGATTGGCGGCTCCA
  TTCTAGCCTCTTTGGGTACCTTTCAACAGATGTGGATTTCCAAGCAAGAATATGAAGAAG
  GAGGGAAGCAGTGTGTAGAAAGAAAATGCCCTTGAGAAAGAGTTCCCAAGCTTCTACCTT
  CCTTTTGTCACCTTACGTTTCATAGCTTTAGTATACTCAGGAAAAGAATGACCATCTTTT
  GTAGAATGTTTATACATTTTTGCATATTTCAATTTCCACTTAAATTTTTTAAAGCTTTAA
  CTGGCTCTATAAATTAAGTTTGTGCTTTCCTTGAAATGCACTTATTCTTATTACAAGCAT
  TTTATAATTTTGTATAAATGTCTATTTTCTCTAAATATTTTGCTTTCAGTAAAATGCTTT
  CCAACTCTGTTTAGTGTATTAATTACCAGTGGATTGGTAGAACTGCTTTTTATTGACTAG
  TAAAAGTTACTGCCTATGCTTTTTACCTTAGGCTTACAGAATTAAATAAAAATTAGCCAT
  TCCAGAAATAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_177989 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGTGTGGCTGAGCTCCGGGGT
  GTGTGGACGCCGCTTTGTTGCCTGAGATGAAGTTGGAGCCCTTGTTTTTGACATTGGATC
  CTATACTGTGAGAGCTGGTTATGCTGGTGAGGACTGCCCCAAGGTGGATTTTCCTACAGC
  TATTGGTATGGTGGTAGAAAGAGATGACGGAAGCACATTAATGGAAATAGATGGCGATAA
  AGGCAAACAAGGCGGTCCCACCTACTACATAGATACTAATGCTCTGCGTGTTCCGAGGGA
  GAATATGGAGGCCATTTCACCTCTAAAAAATGGGATGGTTGAAGACTGGGATAGTTTCCA
  AGCTATTTTGGATCATACCTACAAAATGCATGTCAAATCAGAAGCCAGTCTCCATCCTGT
  TCTCATGTCAGAGGCACCGTGGAATACTAGAGCAAAGAGAGAGAAACTGACAGAGTTAAT
  GTTTGAACACTACAACATCCCTGCCTTCTTCCTTTGCAAAACTGCAGTTTTGACAGCATT
  TGCTAATGGTCGTTCTACTGGGCTGATTTTGGACAGTGGAGCCACTCATACCACTGCAAT
  TCCAGTCCACGATGGCTATGTCCTTCAACAAGGCATTGTGAAATCCCCTCTTGCTGGAGA
  CTNTATTACTATGCAGTGCAGAGAACTCTTCCAAGAATGAATATTGAATTGGTTCCTCCA
  TATATGATTGCAACNAAAGAAGCTGTTCGTGAAGGATCTNCAGCANACTGGAAAAGAAAA
  GAGAAGTTGCCTCANGTTACGAGGGTCTGGCACATTATATGTGTAAATTGTGTATNCCAG
  ATTTTCAAAGCTTCGTACTTCAGTGTCAGATTCACTTATGATGAACAGTGGCTGCACANA
  TGCCACTGTTCATATTGAATCCCCATGGCTACATTGTGATTTGGTGCANAGCGCTAAGAT
  TCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_177989 unedited
  TAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTATATTTCTGGAATGGCTAATTTTTATTTAATTCTGTAAGCCTAAGGGAAAAAGCA
  TAGGCAGTAACTTTTACTAGTCAATAAAAAGCAGTTCTACCAATCCACTGGTAATTAATA
  CACTAAACAGAGTTGGAAAGCATTTTACTGAAAGCAAAATATTTAGAGAAAATAGACATT
  TATACAAAATTATAAAATGCTTGTAATAAAAATAAGGGCATTTCAAGGAAAGCACAAACT
  TAATTTATAGAGCCAGTTAAAGCTTTAAAAAATTTAAGGGGAAATTGAAATATGCAAAAA
  TGTATAAACATTCTACAAAAGATGGGCATTCTTTTCCTGAGTATACTAAAGCTATGAAAC
  GTAAGGGGACAAAAGGAAGGTAAAAGCTTGGGAACTCTTTCTCAAGGGCATTTTCTTTCT
  ACACACTGCTTCCCTCCTTCTTCATATTCTTGCTTGGAAATCCACATCTGTTGAAAGGTA
  CCCAAAGAGGCTAAAATGGAGCCGCCAATCCATGAGCTAAACCTCCGTTCCACTGTTGTA
  TTATTTGCAATCAATTTCAACCGCATACTTGGAGGAGTTTTCTGAGACAGCTCTCTATTC
  AACCTGTCAGTAAAACTCTGTATTAGTGTGTTTCCTCCTGCCACTATTACACTGCCATAG
  AGACCTGGTCTGATGTCAATATCACACATCCCAACACTTGTGGTGACAACATGACTGACT
  CCTAACAATGGGTTTCCTGATAACCCCTTTACATTGGAAGGGTCAAAATATCCTTTCTGG
  ATCTTTAGCGCTCTGCACCAAATCACATTGTAGCCATTGGGGAATCATAATGAACAGTTG
  CATCTGTGCAGCCACTGTCATCATAGTTGGATCTGACCTTGAGTACC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_177989
• Insert Size: 1950 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177989.1, NP_817126.1
• RefSeq Size: 1737 bp
• RefSeq ORF: 1164 bp
• Locus ID: 86
• Cytogenetics: 3q26.33
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'This gene encodes a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. Three transcript variants that encode two different protein isoforms have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region that results in the use of a downstream start codon, compared to variant 1. Variants 2 and 3 both encode isoform b, which has a shorter N-terminus when compared to isoform a. This variant is also known as Nbeta2 and isoform b is also known as hArpNbetaS. Isoform b is found mostly in the cytoplasm.