Phospholipase D2 (PLD2) (NM_002663) Human Tagged ORF Clone

CAT#: RC202042

PLD2 (Myc-DDK-tagged)-Human phospholipase D2 (PLD2)

Product Images

Western blot validation of overexpression lysate (Cat# LY419170) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202042 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLD2
• Synonyms: PLD1C
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202042 representing NM_002663
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCGACCCCTGAGAGCCTCTTCCCCACTGGGGACGAACTGGACTCCAGCCAGCTCCAGATGGAGT
  CCGATGAGGTGGACACCCTGAAGGAGGGAGAGGACCCAGCCGACCGGATGCACCCGTTTCTGGCCATCTA
  TGAGCTTCAGTCTCTGAAAGTGCACCCCTTGGTGTTCGCACCTGGGGTCCCTGTCACAGCCCAGGTGGTG
  GGCACCGAAAGATATACCAGCGGATCCAAGGTGGGAACCTGCACTCTGTATTCTGTCCGCTTGACTCACG
  GCGACTTTTCCTGGACAACCAAGAAGAAATACCGTCATTTTCAGGAGCTGCATCGGGACCTCCTGAGACA
  CAAAGTCTTGATGAGTCTGCTCCCTCTGGCTCGATTTGCCGTTGCCTATTCTCCAGCCCGAGATGCAGGC
  AACAGAGAGATGCCCTCTCTACCCCGGGCAGGTCCTGAGGGCTCCACCAGACATGCAGCCAGCAAACAGA
  AATACCTGGAGAATTACCTCAACTGTCTCTTGACCATGTCTTTCTATCGCAACTACCATGCCATGACAGA
  GTTCCTGGAAGTCAGTCAGCTGTCCTTTATCCCGGACTTGGGCCGCAAAGGACTGGAGGGGATGATCCGG
  AAGCGCTCAGGTGGCCACCGTGTTCCTGGCCTCACCTGCTGTGGCCGAGACCAAGTTTGTTATCGCTGGT
  CCAAGAGGTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGTGCCTCGAGACAGGTGCCATCTCATT
  TGTTCAGCTCTTTGACCCTGGCTTTGAGGTGCAAGTGGGGAAAAGGAGCACGGAGGCACGGCACGGCGTG
  CGGATCGATACCTCCCACAGGTCCTTGATTCTCAAGTGCAGCAGCTACCGGCAGGCACGGTGGTGGGCCC
  AAGAGATCACTGAGCTGGCACAGGGCCCAGGCAGAGACTTCCTACAGCTGCACCGGCATGACAGCTACGC
  CCCACCCCGGCCTGGGACCTTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTTGCTGCTGTGGCAGAT
  GCCATCCTTCGAGCTCAAGAGGAGATTTTCATCACAGACTGGTGGTTGAGTCCTGAGGTTTACCTGAAGC
  GTCCGGCCCATTCAGATGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCGGAGGAAGGTGTCCGTGT
  GTCTATTCTGCTGTTTAAAGAAGTGGAATTGGCCTTGGGCATCAACAGTGGCTATAGCAAGAGGGCGCTG
  ATGCTGCTGCACCCCAACATAAAGGTGATGCGTCACCCAGACCAAGTGACGTTGTGGGCCCATCATGAGA
  AGCTCCTGGTGGTGGACCAAGTGGTAGCATTCCTGGGGGGACTGGACCTTGCCTATGGCCGCTGGGATGA
  CCTGCACTACCGACTGACTGACCTTGGAGACTCCTCTGAATCAGCTGCCTCCCAGCCTCCCACCCCGCGC
  CCAGACTCACCAGCCACCCCAGACCTCTCTCACAACCAATTCTTCTGGCTGGGCAAGGACTACAGCAATC
  TTATCACCAAGGACTGGGTGCAGCTGGACCGGCCTTTCGAAGATTTCATTGACAGGGAGACGACCCCTCG
  GATGCCATGGCGGGACGTTGGGGTGGTCGTCCATGGCCTACCGGCCCGGGACCTTGCCCGGCACTTCATC
  CAGCGCTGGAACTTCACCAAGACCACCAAGGCCAAGTACAAGACTCCCATATACCCCTACCTGCTTCCCA
  AGTCTACCAGCACGGCCAATCAGCTCCCCTTCACACTTCCAGGAGGGCAGTGCACCACCGTACAGGTCTT
  GCGATCAGTGGACCGCTGGTCAGCAGGGACTCTGGAGAACTCCATCCTCAATGCCTACCTGCACACCATC
  AGGGAGAGCCAGCACTTCCTCTACATTGAGAATCAGTTCTTCATTAGCTGCTCAGATGGGCGGACGGTTC
  TGAACAAGGTGGGCGATGAGATTGTGGACAGAATCCTGAAGGCCCACAAACAGGGGTGGTGTTACCGAGT
  CTACGTGCTTTTGCCCTTACTCCCTGGCTTCGAGGGTGACATCTCCACGGGCGGTGGCAACTCCATCCAG
  GCCATTCTGCACTTTACTTACAGGACCCTGTGTCGTGGGGAGTATTCAATCCTGCATCGCCTTAAAGCAG
  CCATGGGGACAGCATGGCGGGACTATATTTCCATCTGCGGGCTTCGTACACACGGAGAGCTGGGCGGGCA
  CCCCGTCTCGGAGCTCATCTACATCCACAGCAAGGTGCTCATCGCAGATGACCGGACAGTCATCATTGGT
  TCTGCAAACATCAATGACCGGAGCTTGCTGGGGAAGCGGGACAGTGAGCTGGCCGTGCTGATCGAGGACA
  CAGAGACGGAACCATCCCTCATGAATGGGGCAGAGTATCAGGCGGGCAGGTTTGCCTTGAGTCTGCGGAA
  GCACTGCTTCGGTGTGATTCTTGGAGCAAATACCCGGCCAGACTTGGATCTCCGAGACCCCATCTGTGAT
  GACTTCTTCCAGTTGTGGCAAGACATGGCTGAGAGCAACGCCAATATCTATGAGCAGATCTTCCGCTGCC
  TGCCATCCAATGCCACGCGTTCCCTGCGGACTCTCCGGGAGTACGTGGCCGTGGAGCCCTTGGCCACGGT
  CAGTCCCCCCTTGGCTCGGTCTGAGCTCACCCAGGTCCAGGGCCACCTGGTCCACTTCCCCCTCAAGTTC
  CTAGAGGATGAGTCTTTGCTGCCCCCGCTGGGTAGCAAGGAGGGCATGATCCCCCTAGAAGTGTGGACA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202042 representing NM_002663
  Red=Cloning site Green=Tags(s)
  
  MTATPESLFPTGDELDSSQLQMESDEVDTLKEGEDPADRMHPFLAIYELQSLKVHPLVFAPGVPVTAQVV
  GTERYTSGSKVGTCTLYSVRLTHGDFSWTTKKKYRHFQELHRDLLRHKVLMSLLPLARFAVAYSPARDAG
  NREMPSLPRAGPEGSTRHAASKQKYLENYLNCLLTMSFYRNYHAMTEFLEVSQLSFIPDLGRKGLEGMIR
  KRSGGHRVPGLTCCGRDQVCYRWSKRWLVVKDSFLLYMCLETGAISFVQLFDPGFEVQVGKRSTEARHGV
  RIDTSHRSLILKCSSYRQARWWAQEITELAQGPGRDFLQLHRHDSYAPPRPGTLARWFVNGAGYFAAVAD
  AILRAQEEIFITDWWLSPEVYLKRPAHSDDWRLDIMLKRKAEEGVRVSILLFKEVELALGINSGYSKRAL
  MLLHPNIKVMRHPDQVTLWAHHEKLLVVDQVVAFLGGLDLAYGRWDDLHYRLTDLGDSSESAASQPPTPR
  PDSPATPDLSHNQFFWLGKDYSNLITKDWVQLDRPFEDFIDRETTPRMPWRDVGVVVHGLPARDLARHFI
  QRWNFTKTTKAKYKTPIYPYLLPKSTSTANQLPFTLPGGQCTTVQVLRSVDRWSAGTLENSILNAYLHTI
  RESQHFLYIENQFFISCSDGRTVLNKVGDEIVDRILKAHKQGWCYRVYVLLPLLPGFEGDISTGGGNSIQ
  AILHFTYRTLCRGEYSILHRLKAAMGTAWRDYISICGLRTHGELGGHPVSELIYIHSKVLIADDRTVIIG
  SANINDRSLLGKRDSELAVLIEDTETEPSLMNGAEYQAGRFALSLRKHCFGVILGANTRPDLDLRDPICD
  DFFQLWQDMAESNANIYEQIFRCLPSNATRSLRTLREYVAVEPLATVSPPLARSELTQVQGHLVHFPLKF
  LEDESLLPPLGSKEGMIPLEVWT
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002663
• ORF Size: 2799 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002663.1, NM_002663.2, NM_002663.3, NM_002663.4, NP_002654.2
• RefSeq Size: 3475 bp
• RefSeq ORF: 2802 bp
• Locus ID: 5338
• Cytogenetics: 17p13.2
• Domains: PX, PLDc, PH
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Metabolic pathways
• MW: 105.8 kDa
• Gene Summary: 'The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]'