Phospholipase D2 (PLD2) (NM_002663) Human Untagged Clone

CAT#: SC118498

PLD2 (untagged)-Human phospholipase D2 (PLD2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLD2
• Synonyms: PLD1C
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118498 sequence for NM_002663 edited (data generated by NextGen Sequencing)
  ATGACGGCGACCCCTGAGAGCCTCTTCCCCACTGGGGACGAACTGGACTCCAGCCAGCTC
  CAGATGGAGTCCGATGAGGTGGACACCCTGAAGGAGGGAGAGGACCCAGCCGACCGGATG
  CACCCGTTTCTGGCCATCTATGAGCTTCAGTCTCTGAAAGTGCACCCCTTGGTGTTCGCA
  CCTGGGGTCCCTGTCACAGCCCAGGTGGTGGGCACCGAAAGATATACCAGCGGATCCAAG
  GTGGGAACCTGCACTCTGTATTCTGTCCGCTTGACTCACGGCGACTTTTCCTGGACAACC
  AAGAAGAAATACCGTCATTTTCAGGAGCTGCATCGGGACCTCCTGAGACACAAAGTCTTG
  ATGAGTCTGCTCCCTCTGGCTCGTTTTGCCGTTGCCTATTCTCCAGCCCGAGATGCAGGC
  AACAGAGAGATGCCCTCTCTACCCCGGGCAGGTCCTGAGGGCTCCACCAGACATGCAGCC
  AGCAAACAGAAATACCTGGAGAATTACCTCAACCGTCTCTTGACCATGTCTTTCTATCGC
  AACTACCATGCCATGACAGAGTTCCTGGAAGTCAGTCAGCTGTCCTTTATCCCGGACTTG
  GGCCGCAAAGGACTGGAGGGGATGATCCGGAAGCGCTCAGGTGGCCACCGTGTTCCTGGC
  CTCACCTGCTGTGGCCGAGACCAAGTTTGTTATCGCTGGTCCAAGAGGTGGCTGGTGGTG
  AAGGACTCCTTCCTGCTGTACATGTGCCTCGAGACAGGTGCCATCTCATTTGTTCAGCTC
  TTTGACCCTGGCTTTGAGGTGCAAGTGGGGAAAAGGAGCACGGAGGCACGGCACGGCGTG
  CGGATCGATACCTCCCACAGGTCCTTGATTCTCAAGTGCAGCAGCTACCGGCAGGCACGG
  TGGTGGGCCCAAGAGATCACTGAGCTGGCACAGGGCCCAGGCAGAGACTTCCTACAGCTG
  CACCGGCATGACAGCTACGCCCCACCCCGGCCTGGGACCTTGGCCCGGTGGTTTGTGAAT
  GGGGCAGGTTACTTTGCTGCTGTGGCAGATGCCATCCTTCGAGCTCAAGAGGAGATTTTC
  ATCACAGACTGGTGGTTGAGTCCTGAGGTTTACCTGAAGCGTCCGGCCCATTCAGATGAC
  TGGAGACTGGACATTATGCTCAAGAGGAAGGCGGAGGAGGGTGTCCGTGTGTCTATTCTG
  CTGTTTAAAGAAGTGGAATTGGCCTTGGGCATCAACAGTGGCTATAGCAAGAGGGCGCTG
  ATGCTGCTGCACCCCAACATAAAGGTGATGCGTCACCCAGACCAAGTGACGTTGTGGGCC
  CATCATGAGAAGCTCCTGGTGGTGGACCAAGTGGTAGCATTCCTGGGGGGACTGGACCTT
  GCCTATGGCCGCTGGGATGACCTGCACTACCGACTGACTGACCTTGGAGACTCCTCTGAA
  TCAGCTGCCTCCCAGCCTCCCACCCCGCGCCCAGACTCACCAGCCACCCCAGACCTCTCT
  CACAACCAATTCTTCTGGCTGGGCAAGGACTACAGCAATCTTATCACCAAGGACTGGGTG
  CAGCTGGACCGGCCTTTCGAAGATTTCATTGACAGGGAGACGACCCCTCGGATGCCATGG
  CGGGACGTTGGGGTGGTCGTCCATGGCCTACCGGCCCGGGACCTTGCCCGGCACTTCATC
  CAGCGCTGGAACTTCACCAAGACCACCAAGGCCAAGTACAAGACTCCCACATACCCCTAC
  CTGCTTCCCAAGTCTACCAGCACGGCCAATCAGCTCCCCTTCACACTTCCAGGAGGGCAG
  TGCACCACCGTACAGGTCTTGCGATCAGTGGACCGCTGGTCAGCAGGGACTCTGGAGAAC
  TCCATCCTCAATGCCTACCTGCACACCATCAGGGGGAGCCAGCACTTCCTCTACATTGAG
  AATCAGTTCTTCATTAGCTGCTCAGATGGGCGGACGGTTCTGAACAAGGTGGGCGATGAG
  ATTGTGGACAGAATCCTGAAGGCCCACAAACAGGGGTGGTGTTACCGAGTCTACGTGCTT
  TTGCCCTTACTCCCTGGCTTCGAGGGTGACATCTCCACGGGCGGTGGCAACTCCATCCAG
  GCCATTCTGCACTTTACTTACAGGACCCTGTGTCGTGGGGAGTATTCAATCCTGCATCGC
  CTTAAAGCAGCCATGGGGACAGCATGGCGGGACTATATTTCCATCTGCGGGCTTCGTACA
  CACGGAGAGCTGGGCGGGCACCCCGTCTCGGAGCTCATCTACATCCACAGCAAGGTGCTC
  ATCGCAGATGACCGGACAGTCATCATTGGTTCTGCAAACATCAATGACCGGAGCTTGCTG
  GGGAAGCGGGACAGTGAGCTGGCCGTGCTGATCGAGGACACAGAGACGGAACCATCCCTC
  ATGAATGGGGCAGAGTATCAGGCGGGCAGGTTTGCCTTGAGTCTGCGGAAGCACTGCTTC
  GGTGTGATTCTTGGAGCAAATACCCGGCCAGACTTGGATCTCCGAGACCCCATCTGTGAT
  GACTTCTTCCAGTTGTGGCAAGACATGGCTGAGAGCAACGCCAATATCTATGAGCAGATC
  TTCCGCTGCCTGCCATCCAATGCCACGCGTTCCCTGCGGACTCTCCGGGAGTACGTGGCC
  GTGGAGCCCTTGGCCACGGTCAGTCCCCCCTTGGCTCGGTCTGAGCTCACCCAGGTCCAG
  GGCCACCTGGTCCACTTCCCCCTCAAGTTCCTAGAGGATGAGTCTTTGCTGCCCCCGCTG
  GGTAGCAAGGAGGGCATGATCCCCCTAGAAGTGTGGACATAG
  
  Clone variation with respect to NM_002663.4
  384 a=>t;1895 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002663 unedited
  TCCCCGCCCCGTTCNCGCTAATGGGCGGTAGCGTGTACGGTGGNGAGTCTATATAAGCAG
  AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA
  TTCGGCACGAGGGGAACCCCCGCGGGCGCTGGCTCCGTCTGCCAGGGATGACGGCGACCC
  CTGAGAGCCTCTTCCCCACTGGGGACGAACTGGACTCCAGCCAGCTCCAGATGGAGTCCG
  ATGAGGTGGACACCCTGAAGGAGGGAGAGGACCCAGCCGACCGGATGCACCCGTTTCTGG
  CCATCTATGAGCTTCAGTCTCTGAAAGTGCACCCCTTGGTGTTCGCACCTGGGGTCCCTG
  TCACAGCCCAGGTGGTGGGCACCGAAAGATATACCAGCGGATCCAAGGTGGGAACCTGCA
  CTCTGTATTCTGTCCGCTTGACTCACGGCGACTTTTCCTGGACAACCAAGAAGAAATACC
  GTCATTTTCAGGAGCTGCATCGGGACCTCCTGAGACACANAGTCTTGATGAGTCTGCTCC
  CTCTGGCTCGTTTTGCCGTTGCCTATTCTCCAGCCCGAGATGCAGGCAACAGAGAGATGC
  CCTCTCTACCCGGGCAGGTCCTGAGGGCTCACCAGACATGCAGCCAGCAAACAGAAATAC
  CTGGAGAATTACCTCAACCGTCTCTTGACCATGTCTTTCTATCGNCACTACCATGCCATG
  ACAGAGTTCCTGGAAGTCAGTCAGCTGTCCTTTATCCCGGACTTGGGCCCCANAGGACTG
  GAGGGGATGATCCGGGAGCGCCTCAGTGGCCCCGTGGTCCTGGCCTACCTGCTGGGGCCG
  AAACCAGTTTGTTATCCTTGGTCCAAAAGGTGGCTGGGGGTGAAGGACTCCTTCTGCTTG
  TCATGGCCCTCGGGAACGGGCCATCTCTATTGTTCACTCTTTGAACCTGGCTTTGGGGGC
  ATGGGGAAAAGAACAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002663 unedited
  ACGTACATTATGNACGCGCCGCATTCAGGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTACACCTTTTTTTATGAAATGCTTATTGAACGGTTTCAAATT
  TACCGTTTTTTAACAAACTGGTTGGAGGGAGTTAATGAGCCCTCCTTCCTTCCCCTGGGG
  GGGGCTGAACCAAAAGGGCCTTCTCCTTCCCCCCACCTTGGGAGCAAAGGCCTGGGAGGG
  CCCTGGGACACGGAGGGGTGTTCAAGGGCAGGAAGAGGAAGGCCACAATGGGTAGTAGGG
  CCTGGGGCAAAACCCCCTTCTCCGGAAATAAGAGGCTTGGCCCTGGTGACCCTGCCACGA
  TCTGGGGTTTTACTTTGCAGCACGGAAAAACCGTTTGCACTGGGCAGCATGGGGGATGAA
  CTCTGGGAATCTGTTCTCCTCCCCAGCCCAGGGGGGGGAAGGCCCTTTTCATCTATTTCT
  CACCTAAGGGACCTTTGGAACACCCCTTTTTCCCTTTATGAATGCCAGCGGCCCCAGATC
  TCAAAGGGCACTGCCCTCAGGACTTGGGGTTAAAGGGCATAGTACCAGACACTGGGGGGC
  ACATCTGCTGTAGACCTCCCCCTGACCGAAGCCTGAAGTATAACCACACTTGTTAAGGGG
  ATCCATGCCGGGCTTGCTAACCATGGGGGGGCATTAAAAGGAGTGATCCTCCCCGGAAAC
  TTGGAGGGGGAAAGTGGGCCCAAGAGAGCCCCTGGCCCCTCGGGATAACCTTCAGAACCA
  AATCCAAACGGGGGTACTCGACCCGGGCCCCAAAGGGCTTCCCCAGCCCCCACCCCTTCG
  CCGGGAAATTCCCCGCGGGGAAAAGGGTTGCCCTTGGGATTGGCTTGCCCCCGGAAAAAT
  TCCGGTTGGAAACTATTT
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002663
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002663.2, NP_002654.2
• RefSeq Size: 3475 bp
• RefSeq ORF: 2802 bp
• Locus ID: 5338
• Cytogenetics: 17p13.2
• Domains: PX, PLDc, PH
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (PLD2A).