Phospholipase D2 (PLD2) (NM_002663) Human Untagged Clone

CAT#: SC118498

PLD2 (untagged)-Human phospholipase D2 (PLD2)


  "NM_002663" in other vectors (7)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PLD2
Synonyms PLD1C
Vector pCMV6-XL6
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC118498 sequence for NM_002663 edited (data generated by NextGen Sequencing)
ATGACGGCGACCCCTGAGAGCCTCTTCCCCACTGGGGACGAACTGGACTCCAGCCAGCTC
CAGATGGAGTCCGATGAGGTGGACACCCTGAAGGAGGGAGAGGACCCAGCCGACCGGATG
CACCCGTTTCTGGCCATCTATGAGCTTCAGTCTCTGAAAGTGCACCCCTTGGTGTTCGCA
CCTGGGGTCCCTGTCACAGCCCAGGTGGTGGGCACCGAAAGATATACCAGCGGATCCAAG
GTGGGAACCTGCACTCTGTATTCTGTCCGCTTGACTCACGGCGACTTTTCCTGGACAACC
AAGAAGAAATACCGTCATTTTCAGGAGCTGCATCGGGACCTCCTGAGACACAAAGTCTTG
ATGAGTCTGCTCCCTCTGGCTCGTTTTGCCGTTGCCTATTCTCCAGCCCGAGATGCAGGC
AACAGAGAGATGCCCTCTCTACCCCGGGCAGGTCCTGAGGGCTCCACCAGACATGCAGCC
AGCAAACAGAAATACCTGGAGAATTACCTCAACCGTCTCTTGACCATGTCTTTCTATCGC
AACTACCATGCCATGACAGAGTTCCTGGAAGTCAGTCAGCTGTCCTTTATCCCGGACTTG
GGCCGCAAAGGACTGGAGGGGATGATCCGGAAGCGCTCAGGTGGCCACCGTGTTCCTGGC
CTCACCTGCTGTGGCCGAGACCAAGTTTGTTATCGCTGGTCCAAGAGGTGGCTGGTGGTG
AAGGACTCCTTCCTGCTGTACATGTGCCTCGAGACAGGTGCCATCTCATTTGTTCAGCTC
TTTGACCCTGGCTTTGAGGTGCAAGTGGGGAAAAGGAGCACGGAGGCACGGCACGGCGTG
CGGATCGATACCTCCCACAGGTCCTTGATTCTCAAGTGCAGCAGCTACCGGCAGGCACGG
TGGTGGGCCCAAGAGATCACTGAGCTGGCACAGGGCCCAGGCAGAGACTTCCTACAGCTG
CACCGGCATGACAGCTACGCCCCACCCCGGCCTGGGACCTTGGCCCGGTGGTTTGTGAAT
GGGGCAGGTTACTTTGCTGCTGTGGCAGATGCCATCCTTCGAGCTCAAGAGGAGATTTTC
ATCACAGACTGGTGGTTGAGTCCTGAGGTTTACCTGAAGCGTCCGGCCCATTCAGATGAC
TGGAGACTGGACATTATGCTCAAGAGGAAGGCGGAGGAGGGTGTCCGTGTGTCTATTCTG
CTGTTTAAAGAAGTGGAATTGGCCTTGGGCATCAACAGTGGCTATAGCAAGAGGGCGCTG
ATGCTGCTGCACCCCAACATAAAGGTGATGCGTCACCCAGACCAAGTGACGTTGTGGGCC
CATCATGAGAAGCTCCTGGTGGTGGACCAAGTGGTAGCATTCCTGGGGGGACTGGACCTT
GCCTATGGCCGCTGGGATGACCTGCACTACCGACTGACTGACCTTGGAGACTCCTCTGAA
TCAGCTGCCTCCCAGCCTCCCACCCCGCGCCCAGACTCACCAGCCACCCCAGACCTCTCT
CACAACCAATTCTTCTGGCTGGGCAAGGACTACAGCAATCTTATCACCAAGGACTGGGTG
CAGCTGGACCGGCCTTTCGAAGATTTCATTGACAGGGAGACGACCCCTCGGATGCCATGG
CGGGACGTTGGGGTGGTCGTCCATGGCCTACCGGCCCGGGACCTTGCCCGGCACTTCATC
CAGCGCTGGAACTTCACCAAGACCACCAAGGCCAAGTACAAGACTCCCACATACCCCTAC
CTGCTTCCCAAGTCTACCAGCACGGCCAATCAGCTCCCCTTCACACTTCCAGGAGGGCAG
TGCACCACCGTACAGGTCTTGCGATCAGTGGACCGCTGGTCAGCAGGGACTCTGGAGAAC
TCCATCCTCAATGCCTACCTGCACACCATCAGGGGGAGCCAGCACTTCCTCTACATTGAG
AATCAGTTCTTCATTAGCTGCTCAGATGGGCGGACGGTTCTGAACAAGGTGGGCGATGAG
ATTGTGGACAGAATCCTGAAGGCCCACAAACAGGGGTGGTGTTACCGAGTCTACGTGCTT
TTGCCCTTACTCCCTGGCTTCGAGGGTGACATCTCCACGGGCGGTGGCAACTCCATCCAG
GCCATTCTGCACTTTACTTACAGGACCCTGTGTCGTGGGGAGTATTCAATCCTGCATCGC
CTTAAAGCAGCCATGGGGACAGCATGGCGGGACTATATTTCCATCTGCGGGCTTCGTACA
CACGGAGAGCTGGGCGGGCACCCCGTCTCGGAGCTCATCTACATCCACAGCAAGGTGCTC
ATCGCAGATGACCGGACAGTCATCATTGGTTCTGCAAACATCAATGACCGGAGCTTGCTG
GGGAAGCGGGACAGTGAGCTGGCCGTGCTGATCGAGGACACAGAGACGGAACCATCCCTC
ATGAATGGGGCAGAGTATCAGGCGGGCAGGTTTGCCTTGAGTCTGCGGAAGCACTGCTTC
GGTGTGATTCTTGGAGCAAATACCCGGCCAGACTTGGATCTCCGAGACCCCATCTGTGAT
GACTTCTTCCAGTTGTGGCAAGACATGGCTGAGAGCAACGCCAATATCTATGAGCAGATC
TTCCGCTGCCTGCCATCCAATGCCACGCGTTCCCTGCGGACTCTCCGGGAGTACGTGGCC
GTGGAGCCCTTGGCCACGGTCAGTCCCCCCTTGGCTCGGTCTGAGCTCACCCAGGTCCAG
GGCCACCTGGTCCACTTCCCCCTCAAGTTCCTAGAGGATGAGTCTTTGCTGCCCCCGCTG
GGTAGCAAGGAGGGCATGATCCCCCTAGAAGTGTGGACATAG

Clone variation with respect to NM_002663.4
384 a=>t;1895 a=>g
>OriGene 5' read for NM_002663 unedited
TCCCCGCCCCGTTCNCGCTAATGGGCGGTAGCGTGTACGGTGGNGAGTCTATATAAGCAG
AGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAA
TTCGGCACGAGGGGAACCCCCGCGGGCGCTGGCTCCGTCTGCCAGGGATGACGGCGACCC
CTGAGAGCCTCTTCCCCACTGGGGACGAACTGGACTCCAGCCAGCTCCAGATGGAGTCCG
ATGAGGTGGACACCCTGAAGGAGGGAGAGGACCCAGCCGACCGGATGCACCCGTTTCTGG
CCATCTATGAGCTTCAGTCTCTGAAAGTGCACCCCTTGGTGTTCGCACCTGGGGTCCCTG
TCACAGCCCAGGTGGTGGGCACCGAAAGATATACCAGCGGATCCAAGGTGGGAACCTGCA
CTCTGTATTCTGTCCGCTTGACTCACGGCGACTTTTCCTGGACAACCAAGAAGAAATACC
GTCATTTTCAGGAGCTGCATCGGGACCTCCTGAGACACANAGTCTTGATGAGTCTGCTCC
CTCTGGCTCGTTTTGCCGTTGCCTATTCTCCAGCCCGAGATGCAGGCAACAGAGAGATGC
CCTCTCTACCCGGGCAGGTCCTGAGGGCTCACCAGACATGCAGCCAGCAAACAGAAATAC
CTGGAGAATTACCTCAACCGTCTCTTGACCATGTCTTTCTATCGNCACTACCATGCCATG
ACAGAGTTCCTGGAAGTCAGTCAGCTGTCCTTTATCCCGGACTTGGGCCCCANAGGACTG
GAGGGGATGATCCGGGAGCGCCTCAGTGGCCCCGTGGTCCTGGCCTACCTGCTGGGGCCG
AAACCAGTTTGTTATCCTTGGTCCAAAAGGTGGCTGGGGGTGAAGGACTCCTTCTGCTTG
TCATGGCCCTCGGGAACGGGCCATCTCTATTGTTCACTCTTTGAACCTGGCTTTGGGGGC
ATGGGGAAAAGAACAC
>OriGene 3' read for NM_002663 unedited
ACGTACATTATGNACGCGCCGCATTCAGGATCGGTTTTTTTTTTTTTTTTTTTTTTTTTT
TTTTTTTTTTTTTTTTTTTACACCTTTTTTTATGAAATGCTTATTGAACGGTTTCAAATT
TACCGTTTTTTAACAAACTGGTTGGAGGGAGTTAATGAGCCCTCCTTCCTTCCCCTGGGG
GGGGCTGAACCAAAAGGGCCTTCTCCTTCCCCCCACCTTGGGAGCAAAGGCCTGGGAGGG
CCCTGGGACACGGAGGGGTGTTCAAGGGCAGGAAGAGGAAGGCCACAATGGGTAGTAGGG
CCTGGGGCAAAACCCCCTTCTCCGGAAATAAGAGGCTTGGCCCTGGTGACCCTGCCACGA
TCTGGGGTTTTACTTTGCAGCACGGAAAAACCGTTTGCACTGGGCAGCATGGGGGATGAA
CTCTGGGAATCTGTTCTCCTCCCCAGCCCAGGGGGGGGAAGGCCCTTTTCATCTATTTCT
CACCTAAGGGACCTTTGGAACACCCCTTTTTCCCTTTATGAATGCCAGCGGCCCCAGATC
TCAAAGGGCACTGCCCTCAGGACTTGGGGTTAAAGGGCATAGTACCAGACACTGGGGGGC
ACATCTGCTGTAGACCTCCCCCTGACCGAAGCCTGAAGTATAACCACACTTGTTAAGGGG
ATCCATGCCGGGCTTGCTAACCATGGGGGGGCATTAAAAGGAGTGATCCTCCCCGGAAAC
TTGGAGGGGGAAAGTGGGCCCAAGAGAGCCCCTGGCCCCTCGGGATAACCTTCAGAACCA
AATCCAAACGGGGGTACTCGACCCGGGCCCCAAAGGGCTTCCCCAGCCCCCACCCCTTCG
CCGGGAAATTCCCCGCGGGGAAAAGGGTTGCCCTTGGGATTGGCTTGCCCCCGGAAAAAT
TCCGGTTGGAAACTATTT
Restriction Sites ECoRI-NOT     
ACCN NM_002663
Insert Size 4000 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002663.2, NP_002654.2
RefSeq Size 3475 bp
RefSeq ORF 2802 bp
Locus ID 5338
Cytogenetics 17p13.2
Domains PX, PLDc, PH
Protein Families Druggable Genome
Protein Pathways Endocytosis, Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Metabolic pathways
Gene Summary 'The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]'
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (PLD2A).

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