Phospholipase D2 (PLD2) (NM_002663) Human Untagged Clone

CAT#: SC324003

PLD2 (untagged)-Human phospholipase D2 (PLD2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLD2
• Synonyms: PLD1C
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002663.2 CTCGGCCGGGGCGTGGGCTCCGGCTGCAGCTCCGGTCTGCTCTCTTGGCTCGGGAACCCC
  CGCGGGCGCTGGCTCCGTCTGCCAGGGATGACGGCGACCCCTGAGAGCCTCTTCCCCACT
  GGGGACGAACTGGACTCCAGCCAGCTCCAGATGGAGTCCGATGAGGTGGACACCCTGAAG
  GAGGGAGAGGACCCAGCCGACCGGATGCACCCGTTTCTGGCCATCTATGAGCTTCAGTCT
  CTGAAAGTGCACCCCTTGGTGTTCGCACCTGGGGTCCCTGTCACAGCCCAGGTGGTGGGC
  ACCGAAAGATATACCAGCGGATCCAAGGTGGGAACCTGCACTCTGTATTCTGTCCGCTTG
  ACTCACGGCGACTTTTCCTGGACAACCAAGAAGAAATACCGTCATTTTCAGGAGCTGCAT
  CGGGACCTCCTGAGACACAAAGTCTTGATGAGTCTGCTCCCTCTGGCTCGATTTGCCGTT
  GCCTATTCTCCAGCCCGAGATGCAGGCAACAGAGAGATGCCCTCTCTACCCCGGGCAGGT
  CCTGAGGGCTCCACCAGACATGCAGCCAGCAAACAGAAATACCTGGAGAATTACCTCAAC
  TGTCTCTTGACCATGTCTTTCTATCGCAACTACCATGCCATGACAGAGTTCCTGGAAGTC
  AGTCAGCTGTCCTTTATCCCGGACTTGGGCCGCAAAGGACTGGAGGGGATGATCCGGAAG
  CGCTCAGGTGGCCACCGTGTTCCTGGCCTCACCTGCTGTGGCCGAGACCAAGTTTGTTAT
  CGCTGGTCCAAGAGGTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGTGCCTCGAG
  ACAGGTGCCATCTCATTTGTTCAGCTCTTTGACCCTGGCTTTGAGGTGCAAGTGGGGAAA
  AGGAGCACGGAGGCACGGCACGGCGTGCGGATCGATACCTCCCACAGGTCCTTGATTCTC
  AAGTGCAGCAGCTACCGGCAGGCACGGTGGTGGGCCCAAGAGATCACTGAGCTGGCACAG
  GGCCCAGGCAGAGACTTCCTACAGCTGCACCGGCATGACAGCTACGCCCCACCCCGGCCT
  GGGACCTTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTTGCTGCTGTGGCAGATGCC
  ATCCTTCGAGCTCAAGAGGAGATTTTCATCACAGACTGGTGGTTGAGTCCTGAGGTTTAC
  CTGAAGCGTCCGGCCCATTCAGATGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCG
  GAGGAAGGTGTCCGTGTGTCTATTCTGCTGTTTAAAGAAGTGGAATTGGCCTTGGGCATC
  AACAGTGGCTATAGCAAGAGGGCGCTGATGCTGCTGCACCCCAACATAAAGGTGATGCGT
  CACCCAGACCAAGTGACGTTGTGGGCCCATCATGAGAAGCTCCTGGTGGTGGACCAAGTG
  GTAGCATTCCTGGGGGGACTGGACCTTGCCTATGGCCGCTGGGATGACCTGCACTACCGA
  CTGACTGACCTTGGAGACTCCTCTGAATCAGCTGCCTCCCAGCCTCCCACCCCGCGCCCA
  GACTCACCAGCCACCCCAGACCTCTCTCACAACCAATTCTTCTGGCTGGGCAAGGACTAC
  AGCAATCTTATCACCAAGGACTGGGTGCAGCTGGACCGGCCTTTCGAAGATTTCATTGAC
  AGGGAGACGACCCCTCGGATGCCATGGCGGGACGTTGGGGTGGTCGTCCATGGCCTACCG
  GCCCGGGACCTTGCCCGGCACTTCATCCAGCGCTGGAACTTCACCAAGACCACCAAGGCC
  AAGTACAAGACTCCCATATACCCCTACCTGCTTCCCAAGTCTACCAGCACGGCCAATCAG
  CTCCCCTTCACACTTCCAGGAGGGCAGTGCACCACCGTACAGGTCTTGCGATCAGTGGAC
  CGCTGGTCAGCAGGGACTCTGGAGAACTCCATCCTCAATGCCTACCTGCACACCATCAGG
  GAGAGCCAGCACTTCCTCTACATTGAGAATCAGTTCTTCATTAGCTGCTCAGATGGGCGG
  ACGGTTCTGAACAAGGTGGGCGATGAGATTGTGGACAGAATCCTGAAGGCCCACAAACAG
  GGGTGGTGTTACCGAGTCTACGTGCTTTTGCCCTTACTCCCTGGCTTCGAGGGTGACATC
  TCCACGGGCGGTGGCAACTCCATCCAGGCCATTCTGCACTTTACTTACAGGACCCTGTGT
  CGTGGGGAGTATTCAATCCTGCATCGCCTTAAAGCAGCCATGGGGACAGCATGGCGGGAC
  TATATTTCCATCTGCGGGCTTCGTACACACGGAGAGCTGGGCGGGCACCCCGTCTCGGAG
  CTCATCTACATCCACAGCAAGGTGCTCATCGCAGATGACCGGACAGTCATCATTGGTTCT
  GCAAACATCAATGACCGGAGCTTGCTGGGGAAGCGGGACAGTGAGCTGGCCGTGCTGATC
  GAGGACACAGAGACGGAACCATCCCTCATGAATGGGGCAGAGTATCAGGCGGGCAGGTTT
  GCCTTGAGTCTGCGGAAGCACTGCTTCGGTGTGATTCTTGGAGCAAATACCCGGCCAGAC
  TTGGATCTCCGAGACCCCATCTGTGATGACTTCTTCCAGTTGTGGCAAGACATGGCTGAG
  AGCAACGCCAATATCTATGAGCAGATCTTCCGCTGCCTGCCATCCAATGCCACGCGTTCC
  CTGCGGACTCTCCGGGAGTACGTGGCCGTGGAGCCCTTGGCCACGGTCAGTCCCCCCTTG
  GCTCGGTCTGAGCTCACCCAGGTCCAGGGCCACCTGGTCCACTTCCCCCTCAAGTTCCTA
  GAGGATGAGTCTTTGCTGCCCCCGCTGGGTAGCAAGGAGGGCATGATCCCCCTAGAAGTG
  TGGACATAGTTGAGGCCCCCGTCAGGGAGAGGTCACCAGCTGCTGTGCCCCACCACGTCT
  GGCTCCCTGCCCCTTAACCCCAAGGACTGAGGGCAGTGCCCTTTGAGATCTGGGGAGGCA
  GGCATTCCTGAAGGGAACTAGAGGTGTTACAGAGGACCCTTACGTGAGAAATAGCTGAAA
  AGGGCACTCCCAACCCTGGGCTGGGGAGGAGGAGAGAGTCCCAGAGCTCATCCCCCCTGC
  TGCCCAGTGCAAACCACTTCTCCATGCTGCAAAGGAGAAGCACAGCTCCTGCCAGGGTGA
  GCAGGGTCAAGCCTCTTATTCCAGGAGAAGGGGGCTCTGCCCCAGGCCCTACTACCCATT
  GTTCCCTTCCTCTTCCTGCCCTTGAACCCCCTCCCTGTCCCAGGGCCCTCCCAGCCCATT
  GCTGCCAAGGTGGAGGGAAGGATAAAGCCACTTCTGGCTTCAGCCCCCACCAGGGGAAGG
  AAGGAGGGCACATTAACTCCCTCCACCAGCCTGCTGACAGACACTAACTTTGTATCCGTT
  CAATAAGCATTTCATAAATAAAGGTGTAGAAAAGGTTAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_002663
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002663.2, NP_002654.2
• RefSeq Size: 3475 bp
• RefSeq ORF: 2802 bp
• Locus ID: 5338
• Cytogenetics: 17p13.2
• Domains: PX, PLDc, PH
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ether lipid metabolism, Fc gamma R-mediated phagocytosis, Glycerophospholipid metabolism, GnRH signaling pathway, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene catalyzes the hydrolysis of phosphatidylcholine to phosphatidic acid and choline. The activity of the encoded enzyme is enhanced by phosphatidylinositol 4,5-bisphosphate and ADP-ribosylation factor-1. This protein localizes to the peripheral membrane and may be involved in cytoskeletal organization, cell cycle control, transcriptional regulation, and/or regulated secretion. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (PLD2A).