SERPINB1 (NM_030666) Human Tagged ORF Clone
CAT#: RC202138
SERPINB1 (Myc-DDK-tagged)-Human serpin peptidase inhibitor, clade B (ovalbumin), member 1 (SERPINB1)
"NM_030666" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SERPINB1 |
Synonyms | EI; ELANH2; HEL-S-27; HEL57; LEI; M/NEI; MNEI; PI-2; PI2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC202138 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCAGCTGAGCTCAGCAAACACCCGCTTCGCCTTGGACCTGTTCCTGGCGTTGAGTGAGAACAATC CGGCTGGAAACATCTTCATCTCTCCCTTCAGCATTTCATCTGCTATGGCCATGGTTTTTCTGGGGACCAG AGGTAACACGGCAGCACAGCTGTCCAAGACTTTCCATTTCAACACGGTTGAAGAGGTTCATTCAAGATTC CAGAGTCTGAATGCTGATATCAACAAACGTGGAGCGTCTTATATTCTGAAACTTGCTAATAGATTATATG GAGAGAAAACTTACAATTTCCTTCCTGAGTTCTTGGTTTCGACTCAGAAAACATATGGTGCTGACCTGGC CAGTGTGGATTTTCAGCATGCCTCTGAAGATGCAAGGAAGACCATAAACCAGTGGGTCAAAGGACAGACA GAAGGAAAAATTCCGGAACTGTTGGCTTCGGGCATGGTTGATAACATGACCAAACTTGTGCTAGTAAATG CCATCTATTTCAAGGGAAACTGGAAGGATAAATTCATGAAAGAAGCCACGACGAATGCACCATTCAGATT GAATAAGAAAGACAGAAAAACTGTGAAAATGATGTATCAGAAGAAAAAATTTGCATATGGCTACATCGAG GACCTTAAGTGCCGTGTGCTGGAACTGCCTTACCAAGGCGAGGAGCTCAGCATGGTCATCCTGCTGCCGG ATGACATTGAGGACGAGTCCACGGGCCTGAAGAAGATTGAGGAACAGTTGACTTTGGAAAAGTTGCATGA GTGGACTAAACCTGAGAATCTCGATTTCATTGAAGTTAATGTCAGCTTGCCCAGGTTCAAACTGGAAGAG AGTTACACTCTCAACTCCGACCTCGCCCGCCTAGGTGTGCAGGATCTCTTTAACAGTAGCAAGGCTGATC TGTCTGGCATGTCAGGAGCCAGAGATATTTTTATATCAAAAATTGTCCACAAGTCATTTGTGGAAGTGAA TGAAGAGGGAACAGAGGCGGCAGCTGCCACAGCAGGCATCGCAACTTTCTGCATGTTGATGCCCGAAGAA AATTTCACTGCCGACCATCCATTCCTTTTCTTTATTCGGCATAATTCCTCAGGTAGCATCCTATTCTTGG GGAGATTTTCTTCCCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC202138 protein sequence
Red=Cloning site Green=Tags(s) MEQLSSANTRFALDLFLALSENNPAGNIFISPFSISSAMAMVFLGTRGNTAAQLSKTFHFNTVEEVHSRF QSLNADINKRGASYILKLANRLYGEKTYNFLPEFLVSTQKTYGADLASVDFQHASEDARKTINQWVKGQT EGKIPELLASGMVDNMTKLVLVNAIYFKGNWKDKFMKEATTNAPFRLNKKDRKTVKMMYQKKKFAYGYIE DLKCRVLELPYQGEELSMVILLPDDIEDESTGLKKIEEQLTLEKLHEWTKPENLDFIEVNVSLPRFKLEE SYTLNSDLARLGVQDLFNSSKADLSGMSGARDIFISKIVHKSFVEVNEEGTEAAAATAGIATFCMLMPEE NFTADHPFLFFIRHNSSGSILFLGRFSSP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_030666 |
ORF Size | 1137 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_030666.1, NM_030666.2, NM_030666.3, NP_109591.1 |
RefSeq Size | 2678 bp |
RefSeq ORF | 1140 bp |
Locus ID | 1992 |
Cytogenetics | 6p25.2 |
Domains | SERPIN |
Protein Families | Druggable Genome |
MW | 42.7 kDa |
Gene Summary | 'The protein encoded by this gene is a member of the serpin family of proteinase inhibitors. Members of this family maintain homeostasis by neutralizing overexpressed proteinase activity through their function as suicide substrates. This protein inhibits the neutrophil-derived proteinases neutrophil elastase, cathepsin G, and proteinase-3 and thus protects tissues from damage at inflammatory sites. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC109680 | SERPINB1 (untagged)-Human serpin peptidase inhibitor, clade B (ovalbumin), member 1 (SERPINB1) |
USD 310.00 |
|
RG202138 | SERPINB1 (GFP-tagged) - Human serpin peptidase inhibitor, clade B (ovalbumin), member 1 (SERPINB1) |
USD 460.00 |
|
RC202138L1 | Lenti ORF clone of Human serpin peptidase inhibitor, clade B (ovalbumin), member 1 (SERPINB1), Myc-DDK-tagged |
USD 768.00 |
|
RC202138L2 | Lenti ORF clone of Human serpin peptidase inhibitor, clade B (ovalbumin), member 1 (SERPINB1), mGFP tagged |
USD 620.00 |
|
RC202138L3 | Lenti ORF clone of Human serpin peptidase inhibitor, clade B (ovalbumin), member 1 (SERPINB1), Myc-DDK-tagged |
USD 620.00 |
|
RC202138L4 | Lenti ORF clone of Human serpin peptidase inhibitor, clade B (ovalbumin), member 1 (SERPINB1), mGFP tagged |
USD 620.00 |
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