PHF11 (NM_001040443) Human Tagged ORF Clone

CAT#: RC202386

PHF11 (Myc-DDK-tagged)-Human PHD finger protein 11 (PHF11), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY421761) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202386 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHF11
• Synonyms: APY; BCAP; IGEL; IGER; IGHER; NY-REN-34; NYREN34
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202386 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAAAAAGGACATGTGCACTCTGCCCCAAAGATGTCGAATATAATGTCCTGTACTTTGCACAATCAG
  AGAATATAGCTGCTCATGAGAATTGTTTGCTGTATTCTTCAGGACTTGTGGAATGTGAGGATCAGGATCC
  ACTTAATCCTGATAGAAGTTTTGATGTGGAATCAGTAAAGAAAGAAATCCAGAGAGGAAGGAAGTTGAAA
  TGCAAATTTTGTCATAAAAGAGGAGCCACCGTGGGATGTGATTTAAAAAACTGTAACAAGAATTACCACT
  TTTTCTGTGCCAAGAAGGACGACGCAGTTCCACAGTCTGATGGAGTTCGAGGAATTTATAAACTGCTTTG
  CCAGCAACATGCTCAATTCCCGATCATCGCTCAAAGTGCTAAATTTTCAGGAGTGAAAAGAAAAAGAGGA
  AGGAAGAAACCCCTCTCAGGCAATCATGTACAGCCACCCGAAACAATGAAATGTAATACATTCATAAGAC
  AAGTGAAAGAAGAGCATGGCAGACACACAGATGCAACTGTGAAAGTTCCTTTTCTTAAGAAATGCAAGGA
  AGCAGGACTTCTTAATTACTTACTTGAAGAAATATTAGACAAAGTTCATTCAATTCCAGAAAAACTCATG
  GATGAGACTACTTCAGAATCAGACTATGAAGAAATCGGGAGTGCACTTTTTGACTGTAGATTGTTCGAAG
  ACACATTTGTAAATTTTCAAGCAGCAATAGAGAAAAAAATTCATGCATCTCAACAAAGGTGGCAGCAGTT
  GAAGGAAGAGATTGAGCTACTTCAGGACTTAAAACAAACCTTGTGCTCTTTTCAAGAAAATAGAGATCTT
  ATGTCAAGTTCTACATCAATATCATCCCTGTCTTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202386 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEKRTCALCPKDVEYNVLYFAQSENIAAHENCLLYSSGLVECEDQDPLNPDRSFDVESVKKEIQRGRKLK
  CKFCHKRGATVGCDLKNCNKNYHFFCAKKDDAVPQSDGVRGIYKLLCQQHAQFPIIAQSAKFSGVKRKRG
  RKKPLSGNHVQPPETMKCNTFIRQVKEEHGRHTDATVKVPFLKKCKEAGLLNYLLEEILDKVHSIPEKLM
  DETTSESDYEEIGSALFDCRLFEDTFVNFQAAIEKKIHASQQRWQQLKEEIELLQDLKQTLCSFQENRDL
  MSSSTSISSLSY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001040443
• ORF Size: 876 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 1649 bp
• RefSeq ORF: 996 bp
• Locus ID: 51131
• Protein Families: Druggable Genome, Transcription Factors
• MW: 33.5 kDa
• Gene Summary: This gene encodes a protein containing a PHD (plant homeodomain) type zinc finger. This gene has been identified in some studies as a candidate gene for asthma. Naturally-occurring readthrough transcription may occur from the upstream SETDB2 (SET domain bifurcated 2) gene to this locus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]