KCNK6 (NM_004823) Human Tagged ORF Clone

CAT#: RC202421

KCNK6 (Myc-DDK-tagged)-Human potassium channel, subfamily K, member 6 (KCNK6)

Product Images

Western blot validation of overexpression lysate (Cat# LY401515) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202421 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified KCNK6 protein (Cat# TP302421). The protein was produced from HEK293T cells transfected with KCNK6 cDNA clone (Cat# RC202421) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNK6
• Synonyms: K2p6.1; KCNK8; TOSS; TWIK-2; TWIK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202421 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGAGGGGCGCGCTTCTGGCGGGCGCCTTGGCCGCGTACGCCGCGTACCTGGTGCTGGGCGCGCTGT
  TGGTGGCGCGGCTGGAGGGGCCGCACGAAGCCAGGCTCCGAGCCGAGCTGGAGACGCTGCGGGCGCAGCT
  GCTTCAGCGCAGCCCGTGTGTGGCTGCCCCCGCCCTGGACGCCTTCGTGGAGCGAGTGCTGGCGGCCGGA
  CGGCTGGGGCGGGTCGTGCTTGCTAACGCTTCGGGGTCCGCCAACGCCTCGGACCCCGCCTGGGACTTCG
  CCTCTGCTCTCTTCTTCGCCAGCACGCTGATCACCACCGTGGGCTATGGGTACACAACGCCACTGACTGA
  TGCGGGCAAGGCCTTCTCCATCGCCTTTGCGCTCCTGGGCGTGCCGACCACCATGCTGCTGCTGACCGCC
  TCAGCCCAGCGCCTGTCACTGCTGCTGACTCACGTGCCCCTGTCTTGGCTGAGCATGCGTTGGGGCTGGG
  ACCCCCGGCGGGCGGCCTGCTGGCACTTGGTGGCCCTGTTGGGGGTCGTAGTGACCGTCTGCTTTCTGGT
  GCCGGCTGTGATCTTTGCCCACCTCGAGGAGGCCTGGAGCTTCTTGGATGCCTTCTACTTCTGCTTTATC
  TCTCTGTCCACCATCGGCCTGGGCGACTACGTGCCCGGGGAGGCCCCTGGCCAGCCCTACCGGGCCCTCT
  ACAAGGTGCTGGTCACAGTCTACCTCTTCCTGGGCCTGGTGGCCATGGTGCTGGTGCTGCAGACCTTCCG
  CCACGTGTCCGACCTCCACGGCCTCACGGAGCTCATCCTGCTGCCCCCTCCGTGCCCTGCCAGTTTCAAT
  GCGGATGAGGACGATCGGGTGGACATCCTGGGCCCCCAGCCGGAGTCGCACCAGCAACTCTCTGCCAGCT
  CCCACACCGACTACGCTTCCATCCCCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202421 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRRGALLAGALAAYAAYLVLGALLVARLEGPHEARLRAELETLRAQLLQRSPCVAAPALDAFVERVLAAG
  RLGRVVLANASGSANASDPAWDFASALFFASTLITTVGYGYTTPLTDAGKAFSIAFALLGVPTTMLLLTA
  SAQRLSLLLTHVPLSWLSMRWGWDPRRAACWHLVALLGVVVTVCFLVPAVIFAHLEEAWSFLDAFYFCFI
  SLSTIGLGDYVPGEAPGQPYRALYKVLVTVYLFLGLVAMVLVLQTFRHVSDLHGLTELILLPPPCPASFN
  ADEDDRVDILGPQPESHQQLSASSHTDYASIPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004823
• ORF Size: 939 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004823.1, NP_004814.1
• RefSeq Size: 2671 bp
• RefSeq ORF: 942 bp
• Locus ID: 9424
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 33.7 kDa
• Gene Summary: This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. This channel protein, considered an open rectifier, is widely expressed. It is stimulated by arachidonic acid, and inhibited by internal acidification and volatile anaesthetics. [provided by RefSeq, Jul 2008]