Semaphorin 3B (SEMA3B) (NM_001005914) Human Tagged ORF Clone

CAT#: RC202875

SEMA3B (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (SEMA3B), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY423670) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202875 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEMA3B
• Synonyms: LUCA-1; SemA; SEMA5; SEMAA; semaV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202875 representing NM_001005914
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCGGGCCGGGGCTGCCGCCGTGATCCCGGGCCTGGCCCTGCTCTGGGCAGTGGGGCTGGGGAGTG
  CCGCCCCCAGCCCCCCACGCCTTCGGCTCTCCTTCCAAGAGCTCCAGGCCTGGCATGGTCTCCAGACTTT
  CAGCCTGGAGCGAACCTGCTGCTACCAGGCCTTGCTGGTGGATGAGGAGCGTGGACGCCTGTTTGTGGGT
  GCCGAGAACCATGTGGCCTCCCTCAACCTGGACAACATCAGCAAGCGGGCCAAGAAGCTGGCCTGGCCGG
  CCCCTGTGGAATGGCGAGAGGAGTGCAACTGGGCAGGGAAGGACATTGGTACTGAGTGCATGAACTTCGT
  GAAGTTGCTGCATGCCTACAACCGCACCCATTTGCTGGCCTGTGGCACGGGAGCCTTCCACCCAACCTGT
  GCCTTTGTGGAAGTGGGCCACCGGGCAGAGGAGCCCGTCCTCCGGCTGGACCCAGGAAGGATAGAGGATG
  GCAAGGGGAAGAGTCCTTATGACCCCAGGCATCGGGCTGCCTCCGTGCTGGTGGGGGAGGAGCTATACTC
  AGGGGTGGCAGCAGACCTCATGGGACGAGACTTTACCATCTTTCGCAGCCTAGGGCAACGTCCAAGTCTC
  CGAACAGAGCCACACGACTCCCGCTGGCTCAATGAGCCCAAGTTTGTCAAGGTATTTTGGATCCCGGAGA
  GCGAGAACCCAGACGACGACAAAATCTACTTCTTCTTTCGTGAGACGGCGGTAGAGGCGGCGCCGGCACT
  GGGACGCCTGTCCGTGTCCCGCGTTGGCCAGATCTGCCGGAACGACGTGGGCGGCCAGCGCAGCCTGGTC
  AACAAGTGGACGACGTTCCTGAAGGCGCGGCTGGTGTGCTCGGTGCCCGGCGTCGAGGGCGACACCCACT
  TCGATCAGCTCCAGGATGTGTTTCTGTTGTCCTCGCGGGACCACCGGACCCCGCTGCTCTATGCCGTCTT
  CTCCACGTCCAGCATCTTCCAGGGCTCTGCGGTGTGCGTGTACAGCATGAACGACGTGCGCCGGGCCTTC
  TTGGGACCCTTTGCACACAAGGAGGGGCCCATGCACCAGTGGGTGTCATACCAGGGTCGCGTCCCCTACC
  CGCGGCCAGGCATGTGCCCCAGCAAGACCTTTGGCACCTTCAGTTCCACCAAGGACTTCCCAGACGATGT
  CATCCAGTTTGCGCGGAACCACCCCCTCATGTACAACTCTGTCCTGCCCACTGGGGGGCGCCCTCTTTTC
  CTACAAGTTGGAGCCAATTACACCTTCACTCAAATTGCCGCGGACCGGGTTGCAGCCGCTGACGGACACT
  ATGACGTCCTCTTCATTGGCACAGACGTTGGCACGGTGCTGAAGGTGATCTCGGTCCCCAAGGGCAGTAG
  GCCCAGCGCAGAGGGGCTGCTCCTGGAGGAGCTGCACGTGTTTGAGGACTCGGCCGCTGTCACCAGCATG
  CAAATTTCTTCCAAGAGGCACCAGCTGTACGTAGCCTCGCGGAGCGCGGTGGCCCAGATCGCGTTGCACC
  GCTGCGCTGCCCACGGCCGCGTCTGCACCGAATGCTGTCTGGCGCGTGACCCCTACTGCGCCTGGGACGG
  GGTCGCGTGCACGCGCTTCCAGCCCAGTGCCAAGAGGCGGTTCCGGCGGCAAGACGTAAGGAATGGCGAC
  CCCAGCACGTTGTGCTCCGGAGACTCGTCTCGTCCCGCGCTGCTGGAACACAAGGTGTTCGGCGTGGAGG
  GCAGCAGCGCCTTTCTGGAGTGTGAGCCCCGCTCGCTGCAGGCGCGCGTGGAGTGGACTTTCCAGCGCGC
  AGGGGTGACAGCCCACACCCAGGTGCTGGCAGAGGAGCGCACCGAGCGCACCGCCCGGGGACTACTGCTG
  CGCAGGCTGCGGCGCCGGGACTCGGGCGTGTACTTGTGCGCCGCCGTCGAGCAGGGCTTTACGCAACCGC
  TGCGTCGCCTGTCGCTGCACGTGTTGAGTGCTACGCAGGCCGAACGACTGGCGCGGGCCGAGGAGGCTGC
  GCCCGCCGCGCCGCCGGGCCCCAAACTCTGGTACCGGGACTTTCTGCAGCTGGTGGAGCCGGGCGGAGGT
  GGCAGCGCGAACTCCCTGCGCATGTGCCGCCCGCAGCCTGCGCTGCAGTCACTGCCCCTGGAGTCGCGGA
  GAAAGGGCCGTAACCGGAGGACCCACGCCCCTGAGCCTCGCGCTGAGCGGGGGCCGCGCAGCGCAACGCA
  CTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202875 representing NM_001005914
  Red=Cloning site Green=Tags(s)
  
  MGRAGAAAVIPGLALLWAVGLGSAAPSPPRLRLSFQELQAWHGLQTFSLERTCCYQALLVDEERGRLFVG
  AENHVASLNLDNISKRAKKLAWPAPVEWREECNWAGKDIGTECMNFVKLLHAYNRTHLLACGTGAFHPTC
  AFVEVGHRAEEPVLRLDPGRIEDGKGKSPYDPRHRAASVLVGEELYSGVAADLMGRDFTIFRSLGQRPSL
  RTEPHDSRWLNEPKFVKVFWIPESENPDDDKIYFFFRETAVEAAPALGRLSVSRVGQICRNDVGGQRSLV
  NKWTTFLKARLVCSVPGVEGDTHFDQLQDVFLLSSRDHRTPLLYAVFSTSSIFQGSAVCVYSMNDVRRAF
  LGPFAHKEGPMHQWVSYQGRVPYPRPGMCPSKTFGTFSSTKDFPDDVIQFARNHPLMYNSVLPTGGRPLF
  LQVGANYTFTQIAADRVAAADGHYDVLFIGTDVGTVLKVISVPKGSRPSAEGLLLEELHVFEDSAAVTSM
  QISSKRHQLYVASRSAVAQIALHRCAAHGRVCTECCLARDPYCAWDGVACTRFQPSAKRRFRRQDVRNGD
  PSTLCSGDSSRPALLEHKVFGVEGSSAFLECEPRSLQARVEWTFQRAGVTAHTQVLAEERTERTARGLLL
  RRLRRRDSGVYLCAAVEQGFTQPLRRLSLHVLSATQAERLARAEEAAPAAPPGPKLWYRDFLQLVEPGGG
  GSANSLRMCRPQPALQSLPLESRRKGRNRRTHAPEPRAERGPRSATHW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001005914
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005914.1, NM_001005914.2, NP_001005914.1
• RefSeq Size: 2986 bp
• RefSeq ORF: 2247 bp
• Locus ID: 7869
• Cytogenetics: 3p21.31
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Axon guidance
• MW: 83.03 kDa
• Gene Summary: The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]