Semaphorin 3B (SEMA3B) (NM_001005914) Human Untagged Clone

CAT#: SC319265

SEMA3B (untagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3B (SEMA3B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEMA3B
• Synonyms: LUCA-1; SemA; SEMA5; SEMAA; semaV
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001005914.1 GCAGAGTCCAGGGCAGCTCAAGGCTCCTCCACACACACACCCGCTGAACCCTGAGCACCC
  TGAGCTGCTGAGATGGGGCGGGCCGGGGCTGCCGCCGTGATCCCGGGCCTGGCCCTGCTC
  TGGGCAGTGGGGCTGGGGAGTGCCGCCCCCAGCCCCCCACGCCTTCGGCTCTCCTTCCAA
  GAGCTCCAGGCCTGGCATGGTCTCCAGACTTTCAGCCTGGAGCGAACCTGCTGCTACCAG
  GCCTTGCTGGTGGATGAGGAGCGTGGACGCCTGTTTGTGGGTGCCGAGAACCATGTGGCC
  TCCCTCAACCTGGACAACATCAGCAAGCGGGCCAAGAAGCTGGCCTGGCCGGCCCCTGTG
  GAATGGCGAGAGGAGTGCAACTGGGCAGGGAAGGACATTGGTACTGAGTGCATGAACTTC
  GTGAAGTTGCTGCATGCCTACAACCGCACCCATTTGCTGGCCTGTGGCACGGGAGCCTTC
  CACCCAACCTGTGCCTTTGTGGAAGTGGGCCACCGGGCAGAGGAGCCCGTCCTCCGGCTG
  GACCCAGGAAGGATAGAGGATGGCAAGGGGAAGAGTCCTTATGACCCCAGGCATCGGGCT
  GCCTCCGTGCTGGTGGGGGAGGAGCTATACTCAGGGGTGGCAGCAGACCTCATGGGACGA
  GACTTTACCATCTTTCGCAGCCTAGGGCAACGTCCAAGTCTCCGAACAGAGCCACACGAC
  TCCCGCTGGCTCAATGAGCCCAAGTTTGTCAAGGTATTTTGGATCCCGGAGAGCGAGAAC
  CCAGACGACGACAAAATCTACTTCTTCTTTCGTGAGACGGCGGTAGAGGCGGCGCCGGCA
  CTGGGACGCCTGTCCGTGTCCCGCGTTGGCCAGATCTGCCGGAACGACGTGGGCGGCCAG
  CGCAGCCTGGTCAACAAGTGGACGACGTTCCTGAAGGCGCGGCTGGTGTGCTCGGTGCCC
  GGCGTCGAGGGCGACACCCACTTCGATCAGCTCCAGGATGTGTTTCTGTTGTCCTCGCGG
  GACCACCGGACCCCGCTGCTCTATGCCGTCTTCTCCACGTCCAGCATCTTCCAGGGCTCT
  GCGGTGTGCGTGTACAGCATGAACGACGTGCGCCGGGCCTTCTTGGGACCCTTTGCACAC
  AAGGAGGGGCCCATGCACCAGTGGGTGTCATACCAGGGTCGCGTCCCCTACCCGCGGCCA
  GGCATGTGCCCCAGCAAGACCTTTGGCACCTTCAGTTCCACCAAGGACTTCCCAGACGAT
  GTCATCCAGTTTGCGCGGAACCACCCCCTCATGTACAACTCTGTCCTGCCCACTGGGGGG
  CGCCCTCTTTTCCTACAAGTTGGAGCCAATTACACCTTCACTCAAATTGCCGCGGACCGG
  GTTGCAGCCGCTGACGGACACTATGACGTCCTCTTCATTGGCACAGACGTTGGCACGGTG
  CTGAAGGTGATCTCGGTCCCCAAGGGCAGTAGGCCCAGCGCAGAGGGGCTGCTCCTGGAG
  GAGCTGCACGTGTTTGAGGACTCGGCCGCTGTCACCAGCATGCAAATTTCTTCCAAGAGG
  CACCAGCTGTACGTAGCCTCGCGGAGCGCGGTGGCCCAGATCGCGTTGCACCGCTGCGCT
  GCCCACGGCCGCGTCTGCACCGAATGCTGTCTGGCGCGTGACCCCTACTGCGCCTGGGAC
  GGGGTCGCGTGCACGCGCTTCCAGCCCAGTGCCAAGAGGCGGTTCCGGCGGCAAGACGTA
  AGGAATGGCGACCCCAGCACGTTGTGCTCCGGAGACTCGTCTCGTCCCGCGCTGCTGGAA
  CACAAGGTGTTCGGCGTGGAGGGCAGCAGCGCCTTTCTGGAGTGTGAGCCCCGCTCGCTG
  CAGGCGCGCGTGGAGTGGACTTTCCAGCGCGCAGGGGTGACAGCCCACACCCAGGTGCTG
  GCAGAGGAGCGCACCGAGCGCACCGCCCGGGGACTACTGCTGCGCAGGCTGCGGCGCCGG
  GACTCGGGCGTGTACTTGTGCGCCGCCGTCGAGCAGGGCTTTACGCAACCGCTGCGTCGC
  CTGTCGCTGCACGTGTTGAGTGCTACGCAGGCCGAACGACTGGCGCGGGCCGAGGAGGCT
  GCGCCCGCCGCGCCGCCGGGCCCCAAACTCTGGTACCGGGACTTTCTGCAGCTGGTGGAG
  CCGGGCGGAGGTGGCAGCGCGAACTCCCTGCGCATGTGCCGCCCGCAGCCTGCGCTGCAG
  TCACTGCCCCTGGAGTCGCGGAGAAAGGGCCGTAACCGGAGGACCCACGCCCCTGAGCCT
  CGCGCTGAGCGGGGGCCGCGCAGCGCAACGCACTGGTGACCAGACTGTCCCCACGCCGGG
  AACCAAGCAGGAGACGACAGGCGAGAGAGGAGCCAGACAGACCCTGAAAAGAAGGACGGG
  TTGGGGCCGGGCACATTGGGGGTCACCGGCCGATGGAGACACCAACCGACAGGCCCTGGC
  TGAGGGCAGCTGCGCGGGCTTATTTATTAACAGGATAACCCTTGAATGTAGCAGCCCCGG
  GAGGGCGGCACAGGTCGGGCGCAGGATTCAGCCGGAGGGAAGGGACGGGGAAGCCGAGCT
  CCAGAGCAACGACCAGGGCCGAGGAGGTGCCTGGAGTGCCCACCCTGGGAGACAGACCCC
  ACCTCCTTGGGTAGTGAGCAGTGAGCAGAAAGCTGTGAACAGGCTGGGCTGCTGGAGGTG
  GGGCGAGGCAGGCCGACTGTACTAAAGTAACGCAATAAACGCATTATCAGCCAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001005914
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001005914.1, NP_001005914.1
• RefSeq Size: 2986 bp
• RefSeq ORF: 2247 bp
• Locus ID: 7869
• Cytogenetics: 3p21.31
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: The protein encoded by this gene belongs to the class-3 semaphorin/collapsin family, whose members function in growth cone guidance during neuronal development. This family member inhibits axonal extension and has been shown to act as a tumor suppressor by inducing apoptosis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. The 5' terminal exon of this variant is inferred based on tiled partial transcript alignments.