ICAD (DFFA) (NM_004401) Human Tagged ORF Clone
CAT#: RC202879L4
- LentiORF®
Lenti ORF clone of Human DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1, mGFP tagged
"NM_004401" in other vectors (4)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | DFFA |
Synonyms | DFF-45; DFF1; ICAD |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC202879).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_004401 |
ORF Size | 993 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_004401.2, NP_004392.1 |
RefSeq Size | 2053 bp |
RefSeq ORF | 996 bp |
Locus ID | 1676 |
Cytogenetics | 1p36.22 |
Domains | CAD |
Protein Pathways | Apoptosis |
MW | 36.6 kDa |
Gene Summary | 'Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC319133 | DFFA (untagged)-Human DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 |
USD 310.00 |
|
RC202879 | DFFA (Myc-DDK-tagged)-Human DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 |
USD 420.00 |
|
RG202879 | DFFA (GFP-tagged) - Human DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1 |
USD 460.00 |
|
RC202879L3 | Lenti ORF clone of Human DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
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