ICAD (DFFA) (NM_004401) Human Untagged Clone

CAT#: SC319133

DFFA (untagged)-Human DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DFFA
• Synonyms: DFF-45; DFF1; ICAD
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004401.2 CGAAGGTGGGCAGGTCCCACCTTGTGGAGGATGGAGGTGACCGGGGACGCCGGGGTACCA
  GAATCTGGCGAGATCCGGACTCTAAAGCCGTGTCTGCTGCGCCGCAACTACAGCCGCGAA
  CAGCACGGCGTGGCCGCCTCCTGCCTCGAAGACCTGAGGAGCAAGGCCTGTGACATTCTG
  GCCATTGATAAGTCCCTGACACCAGTCACCCTGGTCCTGGCAGAGGATGGCACCATAGTG
  GATGATGACGATTACTTTCTGTGTCTACCTTCCAATACTAAGTTTGTGGCATTGGCTAGT
  AATGAGAAATGGGCATACAACAATTCAGATGGAGGTACAGCTTGGATTTCCCAAGAGTCC
  TTTGATGTAGATGAAACAGACAGCGGGGCAGGGTTGAAGTGGAAGAATGTGGCCAGGCAG
  CTGAAAGAAGATCTGTCCAGCATCATCCTCCTATCAGAGGAGGACCTCCAGATGCTTGTT
  GACGCTCCCTGCTCAGACCTGGCTCAGGAACTACGTCAGAGTTGTGCCACCGTCCAGCGG
  CTGCAGCACACACTCCAACAGGTGCTTGACCAAAGAGAGGAAGTGCGTCAGTCCAAGCAG
  CTCCTGCAGCTGTACCTCCAGGCTTTGGAGAAAGAGGGCAGCCTCTTGTCAAAGCAGGAA
  GAGTCCAAAGCTGCCTTTGGTGAGGAGGTGGATGCAGTAGACACGGGTATCAGCAGAGAG
  ACCTCCTCGGACGTTGCGCTGGCGAGCCACATCCTTACTGCACTGAGGGAGAAGCAGGCT
  CCAGAGCTGAGCTTATCTAGTCAGGATTTGGAGTTGGTTACCAAGGAAGACCCCAAAGCA
  CTGGCTGTTGCCTTGAACTGGGACATAAAGAAGACGGAGACTGTTCAGGAGGCCTGTGAG
  TGGGAGCTCGCCCTGCGCCTGCAGCAGACGCAGAGCTTGCATTCTCTCCGGAGCATCTCA
  GCAAGCAAGGCCTCACCACCTGGTGACCTGCAGAATCCTAAGCGAGCCAGACAGGATCCC
  ACATAGCAGCAGCGGGAAGTGTGCCAAGGAAGCTCTGTGGCGTTGTGTTATTGGTAGACA
  CCCTCAGCCTCATCATTTGACTACCTATGTACTACTCTACCCCCTGCCTTAGAGCACCTT
  CCAGAGAAGCTATTCCAGGTCTCAACATACGCCGTTCCACCAATTTTTTTTTTAGCCCCA
  CCAGCTTCAGGACTTCTGCCAATTTTGAATGATATAGCTGCACCAACAATATCCCGCCTC
  CTCTAATTACATATGATGTTCTCTGTTCAAAAGTAATTGGCAGTGATTGGCCAGGCGCAG
  TGGCTCACGCCTGTAATCCCAGCACTGGGAGGCCGAGGGGGGCGGATCGTGAAGTCAGGA
  GATCGAGACCATCCTGGCTAACATGGTGAAGCCCTGTCTCTACTAAAAATACAAAAAAAA
  TTAGCCGGCCATGGTGGCGGGCGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAG
  AATGGCATGAACCTGGGAGGCAGAGCTTGCAGTGAGCTGAGATTGCGCCACTGCACTCCA
  GCCTGGGCAACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAGTAATTGGCAGTG
  ACTATGGGCGCACTGCCTAACATTTAGCCCTGCCCCATATGGAACATGTTAAAAAAAAAA
  AAAGCCAGGCCGAGCGTGTTGGCTCACGCTTGTAATCCCAGCACTTTGGGAGGCCGAGGC
  GGGTGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACAGTGAAACGTGTTTT
  TACTAAAAGTACAAAAAACTAGCTGGGCGTGGTGGCAGGAGCCTGTAGTCCCAGCTACTC
  GGGAGGCTGAGGCAGGAGAATTGCTTGAACCGGGGAGGCAGAAGTTGCAGTGAGCCGAGA
  TTATGCCACTGTACTCCAGCCTGGGTGACAGAGTGACACTCTGTCTCAAAAAAAAAAAAA
  AAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004401
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004401.2, NP_004392.1
• RefSeq Size: 2053 bp
• RefSeq ORF: 996 bp
• Locus ID: 1676
• Cytogenetics: 1p36.22
• Domains: CAD
• Protein Pathways: Apoptosis
• Gene Summary: 'Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1).