ICAD (DFFA) (NM_004401) Human Untagged Clone

CAT#: SC319133

DFFA (untagged)-Human DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1


  "NM_004401" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "DFFA"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol DFFA
Synonyms DFF-45; DFF1; ICAD
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_004401.2 CGAAGGTGGGCAGGTCCCACCTTGTGGAGGATGGAGGTGACCGGGGACGCCGGGGTACCA
GAATCTGGCGAGATCCGGACTCTAAAGCCGTGTCTGCTGCGCCGCAACTACAGCCGCGAA
CAGCACGGCGTGGCCGCCTCCTGCCTCGAAGACCTGAGGAGCAAGGCCTGTGACATTCTG
GCCATTGATAAGTCCCTGACACCAGTCACCCTGGTCCTGGCAGAGGATGGCACCATAGTG
GATGATGACGATTACTTTCTGTGTCTACCTTCCAATACTAAGTTTGTGGCATTGGCTAGT
AATGAGAAATGGGCATACAACAATTCAGATGGAGGTACAGCTTGGATTTCCCAAGAGTCC
TTTGATGTAGATGAAACAGACAGCGGGGCAGGGTTGAAGTGGAAGAATGTGGCCAGGCAG
CTGAAAGAAGATCTGTCCAGCATCATCCTCCTATCAGAGGAGGACCTCCAGATGCTTGTT
GACGCTCCCTGCTCAGACCTGGCTCAGGAACTACGTCAGAGTTGTGCCACCGTCCAGCGG
CTGCAGCACACACTCCAACAGGTGCTTGACCAAAGAGAGGAAGTGCGTCAGTCCAAGCAG
CTCCTGCAGCTGTACCTCCAGGCTTTGGAGAAAGAGGGCAGCCTCTTGTCAAAGCAGGAA
GAGTCCAAAGCTGCCTTTGGTGAGGAGGTGGATGCAGTAGACACGGGTATCAGCAGAGAG
ACCTCCTCGGACGTTGCGCTGGCGAGCCACATCCTTACTGCACTGAGGGAGAAGCAGGCT
CCAGAGCTGAGCTTATCTAGTCAGGATTTGGAGTTGGTTACCAAGGAAGACCCCAAAGCA
CTGGCTGTTGCCTTGAACTGGGACATAAAGAAGACGGAGACTGTTCAGGAGGCCTGTGAG
TGGGAGCTCGCCCTGCGCCTGCAGCAGACGCAGAGCTTGCATTCTCTCCGGAGCATCTCA
GCAAGCAAGGCCTCACCACCTGGTGACCTGCAGAATCCTAAGCGAGCCAGACAGGATCCC
ACATAGCAGCAGCGGGAAGTGTGCCAAGGAAGCTCTGTGGCGTTGTGTTATTGGTAGACA
CCCTCAGCCTCATCATTTGACTACCTATGTACTACTCTACCCCCTGCCTTAGAGCACCTT
CCAGAGAAGCTATTCCAGGTCTCAACATACGCCGTTCCACCAATTTTTTTTTTAGCCCCA
CCAGCTTCAGGACTTCTGCCAATTTTGAATGATATAGCTGCACCAACAATATCCCGCCTC
CTCTAATTACATATGATGTTCTCTGTTCAAAAGTAATTGGCAGTGATTGGCCAGGCGCAG
TGGCTCACGCCTGTAATCCCAGCACTGGGAGGCCGAGGGGGGCGGATCGTGAAGTCAGGA
GATCGAGACCATCCTGGCTAACATGGTGAAGCCCTGTCTCTACTAAAAATACAAAAAAAA
TTAGCCGGCCATGGTGGCGGGCGCCTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAG
AATGGCATGAACCTGGGAGGCAGAGCTTGCAGTGAGCTGAGATTGCGCCACTGCACTCCA
GCCTGGGCAACAGAGCGAGACTCCGTCTCAAAAAAAAAAAAAAAAAAGTAATTGGCAGTG
ACTATGGGCGCACTGCCTAACATTTAGCCCTGCCCCATATGGAACATGTTAAAAAAAAAA
AAAGCCAGGCCGAGCGTGTTGGCTCACGCTTGTAATCCCAGCACTTTGGGAGGCCGAGGC
GGGTGGATCACGAGGTCAGGAGATCGAGACCATCCTGGCTAACACAGTGAAACGTGTTTT
TACTAAAAGTACAAAAAACTAGCTGGGCGTGGTGGCAGGAGCCTGTAGTCCCAGCTACTC
GGGAGGCTGAGGCAGGAGAATTGCTTGAACCGGGGAGGCAGAAGTTGCAGTGAGCCGAGA
TTATGCCACTGTACTCCAGCCTGGGTGACAGAGTGACACTCTGTCTCAAAAAAAAAAAAA
AAAAA
Restriction Sites Please inquire     
ACCN NM_004401
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004401.2, NP_004392.1
RefSeq Size 2053 bp
RefSeq ORF 996 bp
Locus ID 1676
Cytogenetics 1p36.22
Domains CAD
Protein Pathways Apoptosis
Gene Summary 'Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1).

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.