AKR1B10 (NM_020299) Human Tagged ORF Clone
CAT#: RC203177L4
- LentiORF®
Lenti ORF clone of Human aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10), mGFP tagged
"NM_020299" in other vectors (6)
USD 415.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Symbol | AKR1B10 |
Synonyms | AKR1B11; AKR1B12; ALDRLn; ARL-1; ARL1; HIS; HSI |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC203177).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_020299 |
ORF Size | 948 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020299.3, NP_064695.2 |
RefSeq Size | 1610 bp |
RefSeq ORF | 951 bp |
Locus ID | 57016 |
UniProt ID | O60218 |
Cytogenetics | 7q33 |
Domains | aldo_ket_red |
Protein Families | Druggable Genome |
Protein Pathways | Butanoate metabolism, Fructose and mannose metabolism, Linoleic acid metabolism, Metabolic pathways |
MW | 36 kDa |
Gene Summary | This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC311363 | AKR1B10 (untagged)-Human aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10) |
USD 660.00 |
|
RC203177 | AKR1B10 (Myc-DDK-tagged)-Human aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10) |
USD 420.00 |
|
RG203177 | AKR1B10 (GFP-tagged) - Human aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10) |
USD 460.00 |
|
RC203177L1 | Lenti ORF clone of Human aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10), Myc-DDK-tagged |
USD 620.00 |
|
RC203177L2 | Lenti ORF clone of Human aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10), mGFP tagged |
USD 768.00 |
|
RC203177L3 | Lenti ORF clone of Human aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10), Myc-DDK-tagged |
USD 768.00 |
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