FMO2 (NM_001460) Human Tagged ORF Clone
CAT#: RC203237
FMO2 (Myc-DDK-tagged)-Human flavin containing monooxygenase 2 (non-functional) (FMO2)
"NM_001460" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | FMO2 |
Synonyms | FMO1B1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203237 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAAGAAGGTAGCTGTGATTGGAGCTGGGGTCAGTGGCCTAATTTCTCTGAAGTGCTGTGTGGATG AGGGACTTGAGCCCACTTGCTTTGAGAGAACTGAAGATATTGGAGGAGTGTGGAGGTTCAAAGAGAATGT GGAAGATGGCCGAGCAAGTATCTATCAATCTGTCGTTACCAACACCAGCAAAGAAATGTCCTGTTTCAGT GACTTTCCAATGCCTGAAGATTTTCCAAACTTCCTGCATAATTCTAAACTTCTGGAATATTTCAGGATTT TTGCTAAAAAATTTGATCTGCTAAAATATATTCAGTTCCAGACAACTGTCCTTAGTGTGAGAAAATGTCC AGATTTCTCATCCTCTGGCCAATGGAAGGTTGTCACTCAGAGCAACGGCAAGGAGCAGAGTGCTGTCTTT GACGCAGTTATGGTTTGCAGTGGCCACCACATTCTACCTCATATCCCACTGAAGTCATTTCCAGGTATGG AGAGGTTCAAAGGCCAATATTTCCATAGCCGCCAATACAAGCATCCAGATGGATTTGAGGGAAAACGCAT CCTGGTGATTGGAATGGGAAACTCAGGCTCAGATATTGCTGTTGAGCTGAGTAAGAATGCTGCTCAGGTT TTTATCAGCACCAGGCATGGCACCTGGGTCATGAGCCGTATCTCTGAAGATGGCTATCCTTGGGACTCAG TGTTCCACACCCGGTTTCGTTCTATGCTCCGCAATGTACTGCCACGAACAGCTGTAAAATGGATGATAGA ACAACAGATGAATCGGTGGTTCAACCATGAAAATTATGGCCTTGAGCCTCAAAACAAATACATTATGAAG GAACCTGTACTAAATGATGATGTCCCAAGTCGTCTACTCTGTGGAGCCATCAAGGTGAAATCTACAGTGA AAGAGCTCACAGAAACTTCTGCCATCTTTGAGGATGGAACAGTGGAGGAGAACATTGATGTCATCATTTT TGCAACAGGATATAGTTTCTCTTTTCCCTTCCTTGAAGATTCACTCGTTAAAGTAGAGAATAATATGGTC TCACTGTATAAATACATATTCCCCGCTCACCTGGACAAGTCAACCCTCGCGTGCATTGGTCTCATCCAGC CCCTAGGTTCCATTTTCCCAACTGCTGAACTTCAAGCTCGTTGGGTGACAAGAGTTTTCAAAGGCTTGTG TAGCCTGCCCTCAGAGAGAACTATGATGATGGACATTATCAAAAGGAATGAAAAAAGAATTGACCTGTTT GGAGAAAGCCAGAGCCAGACGTTGCAGACCAATTATGTTGACTACTTGGACGAGCTCGCCTTAGAGATAG GTGCGAAGCCAGATTTCTGCTCTCTCTTGTTCAAAGATCCTAAACTGGCTGTGAGACTCTATTTCGGACC CTGCAACTCCTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203237 protein sequence
Red=Cloning site Green=Tags(s) MAKKVAVIGAGVSGLISLKCCVDEGLEPTCFERTEDIGGVWRFKENVEDGRASIYQSVVTNTSKEMSCFS DFPMPEDFPNFLHNSKLLEYFRIFAKKFDLLKYIQFQTTVLSVRKCPDFSSSGQWKVVTQSNGKEQSAVF DAVMVCSGHHILPHIPLKSFPGMERFKGQYFHSRQYKHPDGFEGKRILVIGMGNSGSDIAVELSKNAAQV FISTRHGTWVMSRISEDGYPWDSVFHTRFRSMLRNVLPRTAVKWMIEQQMNRWFNHENYGLEPQNKYIMK EPVLNDDVPSRLLCGAIKVKSTVKELTETSAIFEDGTVEENIDVIIFATGYSFSFPFLEDSLVKVENNMV SLYKYIFPAHLDKSTLACIGLIQPLGSIFPTAELQARWVTRVFKGLCSLPSERTMMMDIIKRNEKRIDLF GESQSQTLQTNYVDYLDELALEIGAKPDFCSLLFKDPKLAVRLYFGPCNSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001460 |
ORF Size | 1413 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001460.1, NM_001460.2, NM_001460.3, NP_001451.1 |
RefSeq Size | 5304 bp |
RefSeq ORF | 1608 bp |
Locus ID | 2327 |
Cytogenetics | 1q24.3 |
Protein Pathways | Drug metabolism - cytochrome P450 |
MW | 53.6 kDa |
Gene Summary | 'This gene encodes a flavin-containing monooxygenase family member. It is an NADPH-dependent enzyme that catalyzes the N-oxidation of some primary alkylamines through an N-hydroxylamine intermediate. However, some human populations contain an allele (FMO2*2A) with a premature stop codon, resulting in a protein that is C-terminally-truncated, has no catalytic activity, and is likely degraded rapidly. This gene is found in a cluster with other related family members on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC126279 | FMO2 (untagged)-Human flavin containing monooxygenase 2 (non-functional) (FMO2) |
USD 310.00 |
|
RG203237 | FMO2 (GFP-tagged) - Human flavin containing monooxygenase 2 (non-functional) (FMO2) |
USD 460.00 |
|
RC203237L1 | Lenti ORF clone of Human flavin containing monooxygenase 2 (non-functional) (FMO2), Myc-DDK-tagged |
USD 620.00 |
|
RC203237L2 | Lenti ORF clone of Human flavin containing monooxygenase 2 (non-functional) (FMO2), mGFP tagged |
USD 620.00 |
|
RC203237L3 | Lenti ORF clone of Human flavin containing monooxygenase 2 (non-functional) (FMO2), Myc-DDK-tagged |
USD 620.00 |
|
RC203237L4 | Lenti ORF clone of Human flavin containing monooxygenase 2 (non-functional) (FMO2), mGFP tagged |
USD 620.00 |
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