FMO2 (NM_001460) Human Tagged ORF Clone

CAT#: RG203237

FMO2 (GFP-tagged) - Human flavin containing monooxygenase 2 (non-functional) (FMO2)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: FMO2
• Synonyms: FMO1B1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203237 representing NM_001460
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAGAAGGTAGCTGTGATTGGAGCTGGGGTCAGTGGCCTAATTTCTCTGAAGTGCTGTGTGGATG
  AGGGACTTGAGCCCACTTGCTTTGAGAGAACTGAAGATATTGGAGGAGTGTGGAGGTTCAAAGAGAATGT
  GGAAGATGGCCGAGCAAGTATCTATCAATCTGTCGTTACCAACACCAGCAAAGAAATGTCCTGTTTCAGT
  GACTTTCCAATGCCTGAAGATTTTCCAAACTTCCTGCATAATTCTAAACTTCTGGAATATTTCAGGATTT
  TTGCTAAAAAATTTGATCTGCTAAAATATATTCAGTTCCAGACAACTGTCCTTAGTGTGAGAAAATGTCC
  AGATTTCTCATCCTCTGGCCAATGGAAGGTTGTCACTCAGAGCAACGGCAAGGAGCAGAGTGCTGTCTTT
  GACGCAGTTATGGTTTGCAGTGGCCACCACATTCTACCTCATATCCCACTGAAGTCATTTCCAGGTATGG
  AGAGGTTCAAAGGCCAATATTTCCATAGCCGCCAATACAAGCATCCAGATGGATTTGAGGGAAAACGCAT
  CCTGGTGATTGGAATGGGAAACTCAGGCTCAGATATTGCTGTTGAGCTGAGTAAGAATGCTGCTCAGGTT
  TTTATCAGCACCAGGCATGGCACCTGGGTCATGAGCCGTATCTCTGAAGATGGCTATCCTTGGGACTCAG
  TGTTCCACACCCGGTTTCGTTCTATGCTCCGCAATGTACTGCCACGAACAGCTGTAAAATGGATGATAGA
  ACAACAGATGAATCGGTGGTTCAACCATGAAAATTATGGCCTTGAGCCTCAAAACAAATACATTATGAAG
  GAACCTGTACTAAATGATGATGTCCCAAGTCGTCTACTCTGTGGAGCCATCAAGGTGAAATCTACAGTGA
  AAGAGCTCACAGAAACTTCTGCCATCTTTGAGGATGGAACAGTGGAGGAGAACATTGATGTCATCATTTT
  TGCAACAGGATATAGTTTCTCTTTTCCCTTCCTTGAAGATTCACTCGTTAAAGTAGAGAATAATATGGTC
  TCACTGTATAAATACATATTCCCCGCTCACCTGGACAAGTCAACCCTCGCGTGCATTGGTCTCATCCAGC
  CCCTAGGTTCCATTTTCCCAACTGCTGAACTTCAAGCTCGTTGGGTGACAAGAGTTTTCAAAGGCTTGTG
  TAGCCTGCCCTCAGAGAGAACTATGATGATGGACATTATCAAAAGGAATGAAAAAAGAATTGACCTGTTT
  GGAGAAAGCCAGAGCCAGACGTTGCAGACCAATTATGTTGACTACTTGGACGAGCTCGCCTTAGAGATAG
  GTGCGAAGCCAGATTTCTGCTCTCTCTTGTTCAAAGATCCTAAACTGGCTGTGAGACTCTATTTCGGACC
  CTGCAACTCCTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203237 representing NM_001460
  Red=Cloning site Green=Tags(s)
  
  MAKKVAVIGAGVSGLISLKCCVDEGLEPTCFERTEDIGGVWRFKENVEDGRASIYQSVVTNTSKEMSCFS
  DFPMPEDFPNFLHNSKLLEYFRIFAKKFDLLKYIQFQTTVLSVRKCPDFSSSGQWKVVTQSNGKEQSAVF
  DAVMVCSGHHILPHIPLKSFPGMERFKGQYFHSRQYKHPDGFEGKRILVIGMGNSGSDIAVELSKNAAQV
  FISTRHGTWVMSRISEDGYPWDSVFHTRFRSMLRNVLPRTAVKWMIEQQMNRWFNHENYGLEPQNKYIMK
  EPVLNDDVPSRLLCGAIKVKSTVKELTETSAIFEDGTVEENIDVIIFATGYSFSFPFLEDSLVKVENNMV
  SLYKYIFPAHLDKSTLACIGLIQPLGSIFPTAELQARWVTRVFKGLCSLPSERTMMMDIIKRNEKRIDLF
  GESQSQTLQTNYVDYLDELALEIGAKPDFCSLLFKDPKLAVRLYFGPCNSY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001460
• ORF Size: 1413 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001460.2, NP_001451.1
• RefSeq Size: 5181 bp
• RefSeq ORF: 1608 bp
• Locus ID: 2327
• Cytogenetics: 1q24.3
• Protein Pathways: Drug metabolism - cytochrome P450
• Gene Summary: 'This gene encodes a flavin-containing monooxygenase family member. It is an NADPH-dependent enzyme that catalyzes the N-oxidation of some primary alkylamines through an N-hydroxylamine intermediate. However, some human populations contain an allele (FMO2*2A) with a premature stop codon, resulting in a protein that is C-terminally-truncated, has no catalytic activity, and is likely degraded rapidly. This gene is found in a cluster with other related family members on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]'