PLK3 (NM_004073) Human Tagged ORF Clone

CAT#: RC203352

PLK3 (Myc-DDK-tagged)-Human polo-like kinase 3 (PLK3)

Product Images

Western blot validation of overexpression lysate (Cat# LY418239) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203352 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLK3
• Synonyms: CNK; FNK; PLK-3; PRK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203352 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCTGCCGCCGGTTTCCTGTCTCCGCGCCCCTTCCAGCGTGCGGCCGCCGCGCCCGCTCCCCCGG
  CCGGGCCCGGGCCGCCTCCGAGTGCCTTGCGCGGACCTGAGCTGGAGATGCTGGCCGGGCTACCGACGTC
  AGACCCCGGGCGCCTCATCACGGACCCGCGCAGCGGCCGCACCTACCTCAAAGGCCGCTTGTTGGGCAAG
  GGGGGCTTCGCCCGCTGCTACGAGGCCACTGACACAGAGACTGGCAGCGCCTACGCTGTCAAAGTCATCC
  CGCAGAGCCGCGTCGCCAAGCCGCATCAGCGCGAGAAGATCCTAAATGAGATTGAGCTGCACCGAGACCT
  GCAGCACCGCCACATCGTGCGTTTTTCGCACCACTTTGAGGACGCTGACAACATCTACATTTTCTTGGAG
  CTCTGCAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGGCACACCCTGTTGGAGCCAGAAGTGCGCT
  ACTACCTGCGGCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGCGCGGCATCTTGCACCGGGACCTCAA
  GTTGGGAAATTTTTTCATCACTGAGAACATGGAACTGAAGGTGGGGGATTTTGGGCTGGCAGCCCGGTTG
  GAGCCTCCGGAGCAGAGGAAGAAGACCATCTGTGGCACCCCCAACTATGTGGCTCCAGAAGTGCTGCTGA
  GACAGGGCCACGGCCCTGAGGCGGATGTATGGTCACTGGGCTGTGTCATGTACACGCTGCTCTGCGGGAG
  CCCTCCCTTTGAGACGGCTGACCTGAAGGAGACGTACCGCTGCATCAAGCAGGTTCACTACACGCTGCCT
  GCCAGCCTCTCACTGCCTGCCCGGCAGCTCCTGGCCGCCATCCTTCGGGCCTCACCCCGAGACCGCCCCT
  CTATTGACCAGATCCTGCGCCATGACTTCTTTACCAAGGGCTACACCCCCGATCGACTCCCTATCAGCAG
  CTGCGTGACAGTCCCAGACCTGACACCCCCCAACCCAGCTAGGAGTCTGTTTGCCAAAGTTACCAAGAGC
  CTCTTTGGCAGAAAGAAGAAGAGTAAGAATCATGCCCAGGAGAGGGATGAGGTCTCCGGTTTGGTGAGCG
  GCCTCATGCGCACATCCGTTGGCCATCAGGATGCCAGGCCAGAGGCTCCAGCAGCTTCTGGCCCAGCCCC
  TGTCAGCCTGGTAGAGACAGCACCTGAAGACAGCTCACCCCGTGGGACACTGGCAAGCAGTGGAGATGGA
  TTTGAAGAAGGTCTGACTGTGGCCACAGTAGTGGAGTCAGCCCTTTGTGCTCTGAGAAATTGTATAGCCT
  TCATGCCCCCAGCGGAACAGAACCCGGCCCCCCTGGCCCAGCCAGAGCCTCTGGTGTGGGTCAGCAAGTG
  GGTTGACTACTCCAATAAGTTCGGCTTTGGGTATCAACTGTCCAGCCGCCGTGTGGCTGTGCTCTTCAAC
  AATGGCACACATATGGCCCTGTCGGCCAACAGAAAGACTGTGCACTACAATCCCACCAGCACAAAGCACT
  TCTCCTTCTCCGTGGGTGCTGTGCCCCGGGCCCTGCAGCCTCAGCTGGGTATCCTGCGGTACTTCGCCTC
  CTACATGGAGCAGCACCTCATGAAGGGTGGAGATCTGCCCAGTGTGGAAGAGGTAGAGGTACCTGCTCCG
  CCCTTGCTGCTGCAGTGGGTCAAGACGGATCAGGCTCTCCTCATGCTGTTTAGTGATGGCACTGTCCAGG
  TGAACTTCTACGGGGACCACACCAAGCTGATTCTCAGTGGCTGGGAGCCCCTCCTTGTGACTTTTGTGGC
  CCGAAATCGTAGTGCTTGTACTTACCTCGCTTCCCACCTTCGGCAGCTGGGCTGCTCTCCAGACCTGCGG
  CAGCGACTCCGCTATGCTCTGCGCCTGCTCCGGGACCGCAGCCCAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203352 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEPAAGFLSPRPFQRAAAAPAPPAGPGPPPSALRGPELEMLAGLPTSDPGRLITDPRSGRTYLKGRLLGK
  GGFARCYEATDTETGSAYAVKVIPQSRVAKPHQREKILNEIELHRDLQHRHIVRFSHHFEDADNIYIFLE
  LCSRKSLAHIWKARHTLLEPEVRYYLRQILSGLKYLHQRGILHRDLKLGNFFITENMELKVGDFGLAARL
  EPPEQRKKTICGTPNYVAPEVLLRQGHGPEADVWSLGCVMYTLLCGSPPFETADLKETYRCIKQVHYTLP
  ASLSLPARQLLAAILRASPRDRPSIDQILRHDFFTKGYTPDRLPISSCVTVPDLTPPNPARSLFAKVTKS
  LFGRKKKSKNHAQERDEVSGLVSGLMRTSVGHQDARPEAPAASGPAPVSLVETAPEDSSPRGTLASSGDG
  FEEGLTVATVVESALCALRNCIAFMPPAEQNPAPLAQPEPLVWVSKWVDYSNKFGFGYQLSSRRVAVLFN
  NGTHMALSANRKTVHYNPTSTKHFSFSVGAVPRALQPQLGILRYFASYMEQHLMKGGDLPSVEEVEVPAP
  PLLLQWVKTDQALLMLFSDGTVQVNFYGDHTKLILSGWEPLLVTFVARNRSACTYLASHLRQLGCSPDLR
  QRLRYALRLLRDRSPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004073
• ORF Size: 1938 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004073.2, NM_004073.3, NP_004064.2
• RefSeq Size: 2369 bp
• RefSeq ORF: 1941 bp
• Locus ID: 1263
• Domains: pkinase, POLO_box, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 71.6 kDa
• Gene Summary: The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis. [provided by RefSeq, Sep 2015]