PLK3 (NM_004073) Human Untagged Clone

CAT#: SC323648

PLK3 (untagged)-Kinase deficient mutant (K91M) of Human polo-like kinase 3 (Drosophila) (PLK3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLK3
• Synonyms: CNK; FNK; PLK-3; PRK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323648 sequence for NM_004073 edited (data generated by NextGen Sequencing)
  ATGGAGCCTGCCGCCGGTTTCCTGTCTCCGCGCCCCTTCCAGCGTGCGGCCGCCGCGCCC
  GCTCCCCCGGCCGGGCCCGGGCCGCCTCCGAGTGCCTTGCGCGGACCTGAGCTGGAGATG
  CTGGCCGGGCTACCGACGTCAGACCCCGGGCGCCTCATCACGGACCCGCGCAGCGGCCGC
  ACCTACCTCAAAGGCCGCTTGTTGGGCAAGGGGGGCTTCGCCCGCTGCTACGAGGCCACT
  GACACAGAGACTGGCAGCGCCTACGCTGTCATGGTCATCCCGCAGAGCCGCGTCGCCAAG
  CCGCATCAGCGCGAGAAGATCCTAAATGAGATTGAGCTGCACCGAGACCTGCAGCACCGC
  CACATCGTGCGTTTTTCGCACCACTTTGAGGACGCTGACAACATCTACATTTTCTTGGAG
  CTCTGCAGCCGAAAGTCCCTGGCCCACATCTGGAAGGCCCGGCACACCCTGTTGGAGCCA
  GAAGTGCGCTACTACCTGCGGCAGATCCTTTCTGGCCTCAAGTACTTGCACCAGCGCGGC
  ATCTTGCACCGGGACCTCAAGTTGGGAAATTTTTTCATCACTGAGAACATGGAACTGAAG
  GTGGGGGATTTTGGGCTGGCAGCCCGGTTGGAGCCTCCGGAGCAGAGGAAGAAGACCATC
  TGTGGCACCCCCAACTATGTGGCTCCAGAAGTGCTGCTGAGACAGGGCCACGGCCCTGAG
  GCGGATGTATGGTCACTGGGCTGTGTCATGTACACGCTGCTCTGCGGGAGCCCTCCCTTT
  GAGACGGCTGACCTGAAGGAGACGTACCGCTGCATCAAGCAGGTTCACTACACGCTGCCT
  GCCAGCCTCTCACTGCCTGCCCGGCAGCTCCTGGCCGCCATCCTTCGGGCCTCACCCCGA
  GACCGCCCCTCTATTGACCAGATCCTGCGCCATGACTTCTTTACCAAGGGCTACACCCCC
  GATCGACTCCCTATCAGCAGCTGCGTGACAGTCCCAGACCTGACACCCCCCAACCCAGCT
  AGGAGTCTGTTTGCCAAAGTTACCAAGAGCCTCTTTGGCAGAAAGAAGAAGAGTAAGAAT
  CATGCCCAGGAGAGGGATGAGGTCTCCGGTTTGGTGAGCGGCCTCATGCGCACATCCGTT
  GGCCATCAGGATGCCAGGCCAGAGGCTCCAGCAGCTTCTGGCCCAGCCCCTGTCAGCCTG
  GTAGAGACAGCACCTGAAGACAGCTCACCCCGTGGGACACTGGCAAGCAGTGGAGATGGA
  TTTGAAGAAGGTCTGACTGTGGCCACAGTAGTGGAGTCAGCCCTTTGTGCTCTGAGAAAT
  TGTATAGCCTTCATGCCCCCAGCGGAACAGAACCCGGCCCCCCTGGCCCAGCCAGAGCCT
  CTGGTGTGGGTCAGCAAGTGGGTTGACTACTCCAATAAGTTCGGCTTTGGGTATCAACTG
  TCCAGCCGCCGTGTGGCTGTGCTCTTCAACGATGGCACACATATGGCCCTGTCGGCCAAC
  AGAAAGACTGTGCACTACAATCCCACCAGCACAAAGCACTTCTCCTTCTCCGTGGGTGCT
  GTGCCCCGGGCCCTGCAGCCTCAGCTGGGTATCCTGCGGTACTTCGCCTCCTACATGGAG
  CAGCACCTCATGAAGGGTGGAGATCTGCCCAGTGTGGAAGAGGTAGAGGTACCTGCTCCG
  CCCTTGCTGCTGCAGTGGGTCAAGACGGATCAGGCTCTCCTCATGCTGTTTAGTGATGGC
  ACTGTCCAGGTGAACTTCTACGGGGACCACACCAAGCTGATTCTCAGTGGCTGGGAGCCC
  CTCCTTGTGACTTTTGTGGCCCGAAATCGTAGTGCTTGTACTTACCTCGCTTCCCACCTT
  CGGCAGCTGGGCTGCTCTCCAGACCTGCGGCAGCGACTCCGCTATGCTCTGCGCCTGCTC
  CGGGACCGCAGCCCAGCCTAG
  
  Clone variation with respect to NM_004073.2
  272 a=>t;273 a=>g
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_004073 unedited
  CCCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCAAATCCAGGCAGC
  GCCACGCGCGGCCGGGGCCGGGCGGAACCGAGAAGCCGGGACCGCGCTGCGACGCGCCGGCCGCATGGAG
  CCTGCCGCCGGTTTCCTGTCTCCGCGCCCCTTCCAGCGTGCGGCCGCCGCGCCCGCTCCCCCGGCCGGGC
  CCGGGCCGCCTCCGAGTGCCTTGCGCGGACCTGAGCTGGAGATGCTGGCCGGGCTACCGACGTGGGAGCC
  GGGGCGCCTCATCACGGACCCGCGCAGCGGCCGCACCTACCTCAAGGCCGCTTGTTGGGCAAGGGGGGGC
  TTCGCCCGCTGGCTACGAGGCACTGACCCGAACCTGCAGCGGCCTACCCTGTATGGTATTCCGCAAAGCC
  TGTCGCCAACCGCATCACCCAAGAGATCTTATGGAATTGGCTGCCCGAACTGGCACCCCCCGACTGTGTG
  TTTCCGACCATTTAAGCG
  
  Kinase domain sequence

  >SC323648 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYYTGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC
  AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACCAGGCAAATCCAGGCAGCGCCAC
  GCGCGGCCGGGGCCGGGCGGAACCGAGAAGCCGGGACCGCGCTGCGACGCGCCGGCCGCATGGAGCCTGC
  CGCCGGTTTCCTGTCTCCGCGCCCCTTCCAGCGTGCGGCCGCCGC
  
• Restriction Sites: Please inquire
• ACCN: NM_004073
• ORF Size: 1941 bp
• Insert Size: 2200
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.

Reference Data

• RefSeq: NM_004073.2, NP_004064.2
• RefSeq Size: 2369
• RefSeq ORF: 1941
• Locus ID: 1263
• Domains: pkinase, POLO_box, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis. [provided by RefSeq, Sep 2015]