EFEMP2 (NM_016938) Human Tagged ORF Clone
CAT#: RC203726
EFEMP2 (Myc-DDK-tagged)-Human EGF containing fibulin-like extracellular matrix protein 2 (EFEMP2), transcript variant 1
"NM_016938" in other vectors (7)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | EFEMP2 |
Synonyms | ARCL1B; FBLN4; MBP1; UPH1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203726 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCCCTGCGCCTCCTGCCTACCCGGGTCTCTACTGCTCTGGGCGCTGCTACTGTTGCTCTTGGGAT CAGCTTCTCCTCAGGATTCTGAAGAGCCCGACAGCTACACGGAATGCACAGATGGCTATGAGTGGGACCC AGACAGCCAGCACTGCCGGGATGTCAACGAGTGTCTGACCATCCCTGAGGCCTGCAAGGGGGAAATGAAG TGCATCAACCACTACGGGGGCTACTTGTGCCTGCCCCGCTCCGCTGCCGTCATCAACGACCTACACGGCG AGGGACCCCCGCCACCAGTGCCTCCCGCTCAACACCCCAACCCCTGCCCACCAGGCTATGAGCCCGACGA TCAGGACAGCTGTGTGGATGTGGACGAGTGTGCCCAGGCCCTGCACGACTGTCGCCCCAGCCAGGACTGC CATAACTTGCCTGGCTCCTATCAGTGCACCTGCCCTGATGGTTACCGCAAGATCGGGCCCGAGTGTGTGG ACATAGACGAGTGCCGCTACCGCTACTGCCAGCACCGCTGCGTGAACCTGCCTGGCTCCTTCCGCTGCCA GTGCGAGCCGGGCTTCCAGCTGGGGCCTAACAACCGCTCCTGTGTTGATGTGAACGAGTGTGACATGGGG GCCCCATGCGAGCAGCGCTGCTTCAACTCCTATGGGACCTTCCTGTGTCGCTGCCACCAGGGCTATGAGC TGCATCGGGATGGCTTCTCCTGCAGTGATATTGATGAGTGTAGCTACTCCAGCTACCTCTGTCAGTACCG CTGCGTCAACGAGCCAGGCCGTTTCTCCTGCCACTGCCCACAGGGTTACCAGCTGCTGGCCACACGCCTC TGCCAAGACATTGATGAGTGTGAGTCTGGTGCGCACCAGTGCTCCGAGGCCCAAACCTGTGTCAACTTCC ATGGGGGCTACCGCTGCGTGGACACCAACCGCTGCGTGGAGCCCTACATCCAGGTCTCTGAGAACCGCTG TCTCTGCCCGGCCTCCAACCCTCTATGTCGAGAGCAGCCTTCATCCATTGTGCACCGCTACATGACCATC ACCTCGGAGCGGAGCGTGCCCGCTGACGTGTTCCAGATCCAGGCGACCTCCGTCTACCCCGGTGCCTACA ATGCCTTTCAGATCCGTGCTGGAAACTCGCAGGGGGACTTTTACATTAGGCAAATCAACAACGTCAGCGC CATGCTGGTCCTCGCCCGGCCGGTGACGGGCCCCCGGGAGTACGTGCTGGACCTGGAGATGGTCACCATG AATTCCCTCATGAGCTACCGGGCCAGCTCTGTACTGAGGCTCACCGTCTTTGTAGGGGCCTACACCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203726 protein sequence
Red=Cloning site Green=Tags(s) MLPCASCLPGSLLLWALLLLLLGSASPQDSEEPDSYTECTDGYEWDPDSQHCRDVNECLTIPEACKGEMK CINHYGGYLCLPRSAAVINDLHGEGPPPPVPPAQHPNPCPPGYEPDDQDSCVDVDECAQALHDCRPSQDC HNLPGSYQCTCPDGYRKIGPECVDIDECRYRYCQHRCVNLPGSFRCQCEPGFQLGPNNRSCVDVNECDMG APCEQRCFNSYGTFLCRCHQGYELHRDGFSCSDIDECSYSSYLCQYRCVNEPGRFSCHCPQGYQLLATRL CQDIDECESGAHQCSEAQTCVNFHGGYRCVDTNRCVEPYIQVSENRCLCPASNPLCREQPSSIVHRYMTI TSERSVPADVFQIQATSVYPGAYNAFQIRAGNSQGDFYIRQINNVSAMLVLARPVTGPREYVLDLEMVTM NSLMSYRASSVLRLTVFVGAYTF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016938 |
ORF Size | 1329 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016938.1, NM_016938.2, NM_016938.3, NM_016938.4, NP_058634.1, NP_058634.2 |
RefSeq Size | 2096 bp |
RefSeq ORF | 1332 bp |
Locus ID | 30008 |
Domains | EGF_CA, EGF, EGF |
Protein Families | Druggable Genome, Secreted Protein |
MW | 49.4 kDa |
Gene Summary | A large number of extracellular matrix proteins have been found to contain variations of the epidermal growth factor (EGF) domain and have been implicated in functions as diverse as blood coagulation, activation of complement and determination of cell fate during development. The protein encoded by this gene contains four EGF2 domains and six calcium-binding EGF2 domains. This gene is necessary for elastic fiber formation and connective tissue development. Defects in this gene are cause of an autosomal recessive cutis laxa syndrome. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jan 2011] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC114145 | EFEMP2 (untagged)-Human EGF containing fibulin-like extracellular matrix protein 2 (EFEMP2), transcript variant 1 |
USD 310.00 |
|
SC322269 | EFEMP2 (untagged)-Human EGF containing fibulin-like extracellular matrix protein 2 (EFEMP2), transcript variant 1 |
USD 760.00 |
|
RG203726 | EFEMP2 (GFP-tagged) - Human EGF containing fibulin-like extracellular matrix protein 2 (EFEMP2), transcript variant 1 |
USD 460.00 |
|
RC203726L1 | Lenti ORF clone of Human EGF containing fibulin-like extracellular matrix protein 2 (EFEMP2), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC203726L2 | Lenti ORF clone of Human EGF containing fibulin-like extracellular matrix protein 2 (EFEMP2), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC203726L3 | Lenti ORF clone of Human EGF containing fibulin-like extracellular matrix protein 2 (EFEMP2), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC203726L4 | Lenti ORF clone of Human EGF containing fibulin-like extracellular matrix protein 2 (EFEMP2), transcript variant 1, mGFP tagged |
USD 620.00 |
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