PCSK7 (NM_004716) Human Tagged ORF Clone

CAT#: RC203896

PCSK7 (Myc-DDK-tagged)-Human proprotein convertase subtilisin/kexin type 7 (PCSK7)

Product Images

Western blot validation of overexpression lysate (Cat# LY401489) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203896 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PCSK7
• Synonyms: LPC; PC7; PC8; SPC7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203896 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGAAGGGGAGGCAGAAAGTGCCACACTTGGATGCCCCCCTGGGCCTGCCCACCTGCCTCTGGCTGG
  AATTAGCCGGGCTCTTCTTACTGGTTCCCTGGGTCATGGGCCTGGCAGGGACAGGTGGGCCTGATGGCCA
  GGGCACAGGGGGGCCGAGCTGGGCTGTGCACCTGGAAAGCCTGGAAGGTGACGGGGAGGAAGAGACTCTG
  GAGCAGCAGGCGGATGCCTTGGCCCAGGCAGCAGGGCTGGTGAATGCTGGACGCATCGGAGAGCTTCAGG
  GGCACTACCTCTTTGTCCAGCCTGCTGGGCACAGGCCGGCCCTGGAGGTGGAGGCCATCCGGCAGCAGGT
  GGAGGCTGTGTTGGCTGGGCATGAAGCTGTGCGCTGGCACTCAGAGCAGAGGCTGCTAAGGCGGGCCAAG
  CGCAGCGTCCACTTCAACGACCCCAAGTACCCGCAGCAATGGCACCTGAATAACCGACGGAGCCCGGGCA
  GGGACATCAACGTGACGGGTGTGTGGGAACGCAATGTGACTGGGCGAGGGGTGACGGTGGTGGTAGTGGA
  TGACGGAGTGGAACACACCATCCAGGACATTGCACCCAACTATAGCCCTGAGGGTAGCTATGACCTCAAC
  TCTAATGACCCTGACCCCATGCCCCACCCGGATGTGGAGAATGGCAACCACCATGGCACGCGATGTGCAG
  GAGAGATCGCGGCTGTGCCCAACAACAGCTTCTGTGCCGTGGGCGTGGCCTACGGGAGCCGCATCGCAGG
  TATCCGGGTACTGGATGGACCTCTCACAGACAGCATGGAGGCAGTGGCGTTCAACAAGCACTATCAGATC
  AATGACATCTACAGCTGCAGCTGGGGACCAGATGACGATGGGAAGACAGTGGATGGCCCCCATCAGCTTG
  GAAAGGCTGCCTTACAACATGGGGTGATTGCTGGTCGCCAGGGCTTTGGGAGCATCTTTGTGGTAGCCAG
  TGGCAACGGAGGCCAACACAACGACAACTGCAACTACGATGGCTACGCCAACTCCATCTACACCGTCACC
  ATAGGAGCTGTGGATGAGGAGGGACGCATGCCTTTCTATGCAGAAGAATGTGCCTCCATGCTGGCAGTCA
  CCTTCAGTGGTGGGGACAAGATGCTTCGGAGCATTGTGACCACTGACTGGGACCTTCAGAAGGGCACTGG
  CTGCACTGAGGGCCACACAGGGACCTCAGCTGCAGCGCCTCTGGCAGCTGGCATGATAGCCTTAATGCTG
  CAGGTGCGGCCCTGCCTCACGTGGCGTGACGTCCAGCACATCATTGTCTTCACAGCCACCCGGTATGAGG
  ATCGCCGTGCAGAGTGGGTCACCAACGAGGCAGGCTTCAGCCATAGCCACCAGCACGGTTTCGGCCTCCT
  CAACGCCTGGAGGCTCGTGAATGCAGCCAAGATCTGGACATCTGTCCCTTACTTAGCATCCTACGTCAGT
  CCCGTGTTAAAAGAAAACAAGGCGATTCCGCAGTCCCCCCGTTCCCTGGAGGTCCTGTGGAATGTCAGCA
  GGATGGACCTGGAGATGTCAGGGCTGAAGACCCTGGAGCATGTGGCAGTGACAGTCTCCATCACTCACCC
  ACGGCGCGGCAGCTTGGAGCTGAAGCTGTTCTGCCCCAGTGGCATGATGTCCCTCATCGGCGCCCCCCGC
  AGCATGGACTCGGATCCCAACGGCTTCAATGACTGGACCTTCTCCACTGTGCGATGCTGGGGGGAGAGAG
  CCCGAGGGACCTACAGGCTTGTCATCAGGGATGTCGGGGATGAGTCATTCCAGGTCGGCATCCTCCGGCA
  ATGGCAGCTGACCCTATATGGCTCTGTGTGGAGTGCAGTAGACATCAGGGACAGACAAAGGCTGTTAGAG
  AGTGCCATGAGTGGAAAATACCTGCACGATGACTTCGCCCTGCCCTGCCCACCGGGGCTGAAAATTCCTG
  AGGAAGATGGTTACACCATCACCCCCAACACCCTCAAGACCCTGGTGCTGGTAGGCTGTTTCACCGTCTT
  CTGGACTGTTTACTACATGCTGGAAGTATATTTGAGCCAGAGGAATGTGGCTTCCAATCAAGTTTGTAGG
  AGTGGACCCTGCCACTGGCCCCATCGGAGCCGGAAAGCCAAGGAGGAAGGGACAGAGCTAGAATCAGTGC
  CACTTTGCAGCAGCAAGGATCCAGACGAAGTGGAAACAGAGAGCAGGGGCCCTCCCACCACCTCTGACCT
  CCTTGCCCCAGACCTGCTGGAGCAAGGGGACTGGAGCCTGTCCCAGAACAAGAGCGCCCTGGACTGCCCT
  CATCAGCACCTAGACGTACCGCACGGGAAGGAGGAGCAGATCTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203896 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPKGRQKVPHLDAPLGLPTCLWLELAGLFLLVPWVMGLAGTGGPDGQGTGGPSWAVHLESLEGDGEEETL
  EQQADALAQAAGLVNAGRIGELQGHYLFVQPAGHRPALEVEAIRQQVEAVLAGHEAVRWHSEQRLLRRAK
  RSVHFNDPKYPQQWHLNNRRSPGRDINVTGVWERNVTGRGVTVVVVDDGVEHTIQDIAPNYSPEGSYDLN
  SNDPDPMPHPDVENGNHHGTRCAGEIAAVPNNSFCAVGVAYGSRIAGIRVLDGPLTDSMEAVAFNKHYQI
  NDIYSCSWGPDDDGKTVDGPHQLGKAALQHGVIAGRQGFGSIFVVASGNGGQHNDNCNYDGYANSIYTVT
  IGAVDEEGRMPFYAEECASMLAVTFSGGDKMLRSIVTTDWDLQKGTGCTEGHTGTSAAAPLAAGMIALML
  QVRPCLTWRDVQHIIVFTATRYEDRRAEWVTNEAGFSHSHQHGFGLLNAWRLVNAAKIWTSVPYLASYVS
  PVLKENKAIPQSPRSLEVLWNVSRMDLEMSGLKTLEHVAVTVSITHPRRGSLELKLFCPSGMMSLIGAPR
  SMDSDPNGFNDWTFSTVRCWGERARGTYRLVIRDVGDESFQVGILRQWQLTLYGSVWSAVDIRDRQRLLE
  SAMSGKYLHDDFALPCPPGLKIPEEDGYTITPNTLKTLVLVGCFTVFWTVYYMLEVYLSQRNVASNQVCR
  SGPCHWPHRSRKAKEEGTELESVPLCSSKDPDEVETESRGPPTTSDLLAPDLLEQGDWSLSQNKSALDCP
  HQHLDVPHGKEEQIC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004716
• ORF Size: 2355 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004716.1, NM_004716.2, NM_004716.3, NP_004707.2
• RefSeq Size: 3940 bp
• RefSeq ORF: 2358 bp
• Locus ID: 9159
• Domains: Peptidase_S8, P_proprotein
• Protein Families: Druggable Genome, Protease, Transmembrane
• MW: 86.2 kDa
• Gene Summary: This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. This gene encodes one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. It can process proalbumin and is thought to be responsible for the activation of HIV envelope glycoproteins gp160 and gp140. This gene has been implicated in the transcriptional regulation of housekeeping genes and plays a role in the regulation of iron metabolism. A t(11;14)(q23;q32) chromosome translocation associated with B-cell lymphoma occurs between this gene and its inverted counterpart. [provided by RefSeq, Feb 2014]