PHLPP (PHLPP1) (NM_194449) Human Tagged ORF Clone

CAT#: RC203930L1

Lenti ORF clone of Human PH domain and leucine rich repeat protein phosphatase 1 (PHLPP1), Myc-DDK-tagged

Product Images

Double digestion of RC203930L1 using SgfI and MluI

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  Double digestion of RC203930L1 using SgfI and MluI

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PHLPP1
• Synonyms: PHLPP; PLEKHE1; PPM3A; SCOP
• Vector: pLenti-C-Myc-DDK
• E. coli Selection: Chloramphenicol (34 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC203930).
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_194449
• ORF Size: 3615 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_194449.1, NP_919431.1
• RefSeq Size: 4798 bp
• RefSeq ORF: 5154 bp
• Locus ID: 23239
• UniProt ID: O60346
• Cytogenetics: 18q21.33
• MW: 184.5 kDa
• Gene Summary: This gene encodes a member of the serine/threonine phosphatase family. The encoded protein promotes apoptosis by dephosphorylating and inactivating the serine/threonine kinase Akt, and functions as a tumor suppressor in multiple types of cancer. Increased expression of this gene may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling. [provided by RefSeq, Dec 2011]