DP1 (TFDP1) (NM_007111) Human Tagged ORF Clone

CAT#: RC203986

TFDP1 (Myc-DDK-tagged)-Human transcription factor Dp-1 (TFDP1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY416191) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC203986 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified TFDP1 protein (Cat# TP303986). The protein was produced from HEK293T cells transfected with TFDP1 cDNA clone (Cat# RC203986) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TFDP1
• Synonyms: DILC; Dp-1; DP1; DRTF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203986 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAAAAGATGCCGGTCTAATTGAAGCCAACGGAGAACTCAAGGTCTTCATAGACCAGAACCTTAGTC
  CCGGGAAAGGCGTGGTGTCCCTCGTGGCCGTTCACCCCTCCACCGTCAACCCGCTCGGGAAGCAGCTCTT
  GCCAAAAACCTTTGGACAGTCCAATGTCAACATTGCCCAGCAAGTGGTAATTGGTACGCCTCAGAGACCG
  GCAGCGTCAAACACCCTGGTGGTAGGAAGCCCACACACCCCCAGCACTCACTTTGCCTCTCAGAACCAGC
  CTTCCGACTCCTCACCTTGGTCTGCCGGGAAGCGCAACAGGAAAGGAGAGAAGAATGGCAAGGGCCTACG
  GCATTTCTCCATGAAGGTCTGCGAGAAGGTGCAGAGGAAAGGGACCACTTCCTACAACGAAGTGGCAGAC
  GAGCTGGTTGCGGAGTTCAGTGCTGCCGACAACCACATCTTACCAAACGAGTCAGCTTATGACCAGAAAA
  ACATAAGACGGCGCGTCTACGATGCCTTAAACGTGCTAATGGCCATGAACATCATCTCCAAGGAGAAGAA
  GGAGATCAAGTGGATTGGTCTGCCCACCAACTCGGCTCAGGAATGTCAGAACTTAGAGGTGGAAAGACAG
  AGGAGACTTGAAAGAATAAAACAGAAACAGTCTCAACTTCAAGAACTTATTCTACAGCAAATTGCCTTCA
  AGAACCTGGTGCAGAGAAACCGGCATGCGGAGCAGCAGGCCAGCCGGCCACCGCCACCCAACTCAGTCAT
  CCACCTGCCCTTCATCATCGTCAACACCAGCAAGAAGACGGTCATCGACTGCAGCATCTCCAATGACAAA
  TTTGAGTATCTGTTTAATTTTGACAACACATTTGAAATCCACGATGACATAGAAGTGCTGAAGCGGATGG
  GCATGGCTTGCGGGCTGGAGTCGGGGAGCTGCTCTGCCGAAGACCTTAAAATGGCCAGAAGTCTGGTCCC
  CAAGGCTCTGGAGCCATACGTGACAGAAATGGCTCAGGGAACTGTTGGAGGCGTGTTCATCACGACGGCA
  GGTTCCACGTCTAACGGCACAAGGTTCTCTGCCAGTGACCTGACCAACGGTGCAGATGGGATGCTGGCCA
  CAAGCTCCAATGGGTCTCAGTACAGCGGCTCCAGGGTGGAGACTCCGGTGTCCTACGTCGGGGAGGACGA
  CGAGGAGGACGATGACTTCAACGAGAATGACGAGGACGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203986 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAKDAGLIEANGELKVFIDQNLSPGKGVVSLVAVHPSTVNPLGKQLLPKTFGQSNVNIAQQVVIGTPQRP
  AASNTLVVGSPHTPSTHFASQNQPSDSSPWSAGKRNRKGEKNGKGLRHFSMKVCEKVQRKGTTSYNEVAD
  ELVAEFSAADNHILPNESAYDQKNIRRRVYDALNVLMAMNIISKEKKEIKWIGLPTNSAQECQNLEVERQ
  RRLERIKQKQSQLQELILQQIAFKNLVQRNRHAEQQASRPPPPNSVIHLPFIIVNTSKKTVIDCSISNDK
  FEYLFNFDNTFEIHDDIEVLKRMGMACGLESGSCSAEDLKMARSLVPKALEPYVTEMAQGTVGGVFITTA
  GSTSNGTRFSASDLTNGADGMLATSSNGSQYSGSRVETPVSYVGEDDEEDDDFNENDEDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007111
• ORF Size: 1230 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007111.1, NM_007111.2, NM_007111.3, NM_007111.4, NP_009042.1
• RefSeq Size: 2651 bp
• RefSeq ORF: 1233 bp
• Locus ID: 7027
• Cytogenetics: 13q34
• Domains: E2F_TDP
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cell cycle, TGF-beta signaling pathway
• MW: 45.1 kDa
• Gene Summary: 'This gene encodes a member of a family of transcription factors that heterodimerize with E2F proteins to enhance their DNA-binding activity and promote transcription from E2F target genes. The encoded protein functions as part of this complex to control the transcriptional activity of numerous genes involved in cell cycle progression from G1 to S phase. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 1, 15, and X.[provided by RefSeq, Jan 2009]'