PARL (NM_018622) Human Tagged ORF Clone

CAT#: RC204096

PARL (Myc-DDK-tagged)-Human presenilin associated, rhomboid-like (PARL), nuclear gene encoding mitochondrial protein, transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY402698) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204096 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PARL
• Synonyms: PRO2207; PSARL; PSARL1; PSENIP2; RHBDS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204096 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTGGCGAGGCTGGGCGCAGAGAGGCTGGGGCTGCGGCCAGGCGTGGGGTGCGTCGGTGGGCGGCC
  GCAGCTGCGAGGAGCTCACTGCGGTCCTAACCCCGCCGCAGCTCCTCGGACGCAGGTTTAACTTCTTTAT
  TCAACAAAAATGCGGATTCAGAAAAGCACCCAGGAAGGTTGAACCTCGAAGATCAGACCCAGGGACAAGT
  GGTGAAGCATACAAGAGAAGTGCTTTGATTCCTCCTGTGGAAGAAACAGTCTTTTATCCTTCTCCCTATC
  CTATAAGGAGTCTCATAAAACCTTTATTTTTTACTGTTGGGTTTACAGGCTGTGCATTTGGATCAGCTGC
  TATTTGGCAATATGAATCACTGAAATCCAGGGTCCAGAGTTATTTTGATGGTATAAAAGCTGATTGGTTG
  GATAGCATAAGACCACAAAAAGAAGGAGACTTCAGAAAGGAGATTAACAAGTGGTGGAATAACCTAAGTG
  ATGGCCAGCGGACTGTGACAGGTATTATAGCTGCAAATGTCCTTGTATTCTGTTTATGGAGAGTACCTTC
  TCTGCAGCGGACAATGATCAGATATTTCACATCGAATCCAGCCTCAAAGGTCCTTTGTTCTCCAATGTTG
  CTGTCAACATTCAGTCATTTCTCCTTATTTCACATGGCAGCAAATATGTATGTTTTGTGGAGCTTCTCTT
  CCAGCATAGTGAACATTCTGGGTCAAGAGCAGTTCATGGCAGTGTACCTATCTGCAGGTGTTATTTCCAA
  TTTTGTCAGTTACGTGGGTAAAGTTGCCACAGGAAGATATGGACCATCACTTGGTGCATCTGGTGCCATC
  ATGACAGTCCTCGCAGCTGTCTGCACTAAGATCCCAGAAGGGAGGCTTGCCATTATTTTCCTTCCGATGT
  TCACGTTCACAGCAGGGAATGCCCTGAAAGCCATTATCGCCATGGATACAGCAGGAATGATCCTGGGATG
  GAAATTTTTTGATCATGCGGCACATCTTGGGGGAGCTCTTTTTGGAATATGGTATGTTACTTACGGTCAT
  GAACTGATTTGGAAGAACAGGGAGCCGCTAGTGAAAATCTGGCATGAAATAAGGACTAATGGCCCCAAAA
  AAGGAGGTGGCTCTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204096 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAWRGWAQRGWGCGQAWGASVGGRSCEELTAVLTPPQLLGRRFNFFIQQKCGFRKAPRKVEPRRSDPGTS
  GEAYKRSALIPPVEETVFYPSPYPIRSLIKPLFFTVGFTGCAFGSAAIWQYESLKSRVQSYFDGIKADWL
  DSIRPQKEGDFRKEINKWWNNLSDGQRTVTGIIAANVLVFCLWRVPSLQRTMIRYFTSNPASKVLCSPML
  LSTFSHFSLFHMAANMYVLWSFSSSIVNILGQEQFMAVYLSAGVISNFVSYVGKVATGRYGPSLGASGAI
  MTVLAAVCTKIPEGRLAIIFLPMFTFTAGNALKAIIAMDTAGMILGWKFFDHAAHLGGALFGIWYVTYGH
  ELIWKNREPLVKIWHEIRTNGPKKGGGSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018622
• ORF Size: 1137 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018622.2, NM_018622.3, NM_018622.4, NM_018622.5, NM_018622.6, NP_061092.3
• RefSeq Size: 1423 bp
• RefSeq ORF: 1140 bp
• Locus ID: 55486
• Domains: Rhomboid
• Protein Families: Protease, Transmembrane
• MW: 42.2 kDa
• Gene Summary: This gene encodes a member of the rhomboid family of intramembrane serine proteases that is localized to the inner mitochondrial membrane. The encoded protein regulates mitochondrial remodeling and apoptosis through regulated substrate proteolysis. Proteolytic processing of the encoded protein results in the release of a small peptide, P-beta, which may transit to the nucleus. Mutations in this gene may be associated with Parkinson's disease. [provided by RefSeq, May 2016]