Cyclin F (CCNF) (NM_001761) Human Tagged ORF Clone

CAT#: RC204189

CCNF (Myc-DDK-tagged)-Human cyclin F (CCNF)

Product Images

Western blot validation of overexpression lysate (Cat# LY419758) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204189 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCNF
• Synonyms: FBX1; FBXO1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204189 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGAGCGGCGGCGTGGTCCACTGTAGGTGTGCCAAGTGTTTCTGTTATCCTACAAAGCGAAGAATAA
  GGAGGAGGCCCCGAAACCTGACCATCTTGAGTCTCCCCGAAGATGTGCTCTTTCACATCCTGAAATGGCT
  TTCTGTAGAGGACATCCTGGCCGTCCGAGCTGTACACTCCCAGCTGAAGGACCTGGTGGACAACCACGCC
  AGTGTGTGGGCATGTGCCAGCTTCCAGGAGCTGTGGCCGTCTCCAGGGAACCTGAAGCTCTTTGAAAGGG
  CTGCTGAAAAGGGGAATTTCGAAGCTGCTGTGAAGCTGGGCATAGCCTACCTCTACAATGAAGGCCTGTC
  TGTGTCTGATGAGGCCCGCGCAGAAGTGAATGGCCTGAAGGCCTCTCGCTTCTTCAGTCTCGCTGAGCGG
  CTGAATGTGGGTGCCGCACCTTTCATCTGGCTCTTCATCCGCCCTCCGTGGTCGGTGAGCGGAAGCTGCT
  GCAAGGCCGTGGTTCACGAGAGCCTCAGGGCAGAGTGCCAGCTGCAGAGGACTCACAAAGCATCCATATT
  GCACTGCTTGGGCAGAGTGCTGAGTCTGTTCGAGGATGAGGAGAAGCAGCAGCAGGCCCATGACCTGTTT
  GAGGAGGCTGCTCATCAGGGATGTCTGACCAGCTCCTACCTCCTCTGGGAAAGCGACAGGAGGACAGATG
  TGTCAGATCCTGGGCGATGCCTCCACAGCTTCCGAAAACTCAGGGACTACGCTGCCAAAGGCTGCTGGGA
  AGCGCAGCTGTCTTTAGCCAAAGCCTGTGCAAATGCAAACCAGCTTGGACTGGAGGTGAGAGCTTCCAGT
  GAGATCGTCTGCCAGCTATTTCAGGCTTCCCAGGCTGTCAGTAAACAACAAGTCTTCTCCGTGCAGAAGG
  GACTCAATGACACAATGAGGTACATTCTGATCGACTGGCTGGTGGAAGTTGCCACCATGAAGGACTTCAC
  AAGCCTGTGCCTGCACCTGACCGTGGAGTGTGTGGACCGGTACCTGCGGAGGAGGCTGGTGCCGCGGTAC
  AGGCTCCAGCTGCTGGGCATCGCCTGCATGGTCATCTGCACCCGGTTTATCAGTAAAGAGATCCTGACCA
  TCCGGGAGGCCGTATGGCTCACGGACAACACTTACAAGTACGAGGACCTGGTGAGAATGATGGGCGAGAT
  CGTCTCCGCCTTGGAAGGGAAGATTCGAGTCCCCACTGTGGTGGATTACAAGGAGGTCCTGCTGACGCTA
  GTCCCTGTGGAGCTGAGAACCCAGCACCTGTGCAGCTTCCTCTGCGAGCTCTCCCTGCTGCACACCAGCC
  TGTCCGCCTACGCCCCAGCCCGCCTGGCTGCCGCAGCCCTGCTCCTGGCCAGACTGACGCACGGGCAGAC
  ACAGCCCTGGACCACTCAGCTGTGGGACCTCACCGGATTCTCCTATGAAGACCTCATTCCCTGCGTCTTG
  AGCCTCCATAAGAAGTGCTTCCATGATGACGCCCCCAAGGACTACAGGCAAGTCTCTCTGACCGCCGTGA
  AGCAGCGGTTTGAGGACAAGCGCTATGGAGAAATCAGCCAGGAAGAGGTGCTGAGCTACAGCCAGTTGTG
  TGCTGCATTAGGAGTGACACAAGACAGCCCCGACCCCCCGACTTTCCTCAGCACAGGGGAGATCCACGCC
  TTCCTCAGCTCTCCCTCGGGGCGGAGAACCAAACGGAAGCGGGAGAACAGCCTCCAGGAAGACAGAGGCA
  GCTTCGTTACCACCCCCACTGCGGAGCTGTCCAGCCAGGAGGAGACGCTGCTGGGCAGCTTCCTCGACTG
  GAGCCTGGACTGCTGCTCTGGCTATGAAGGCGACCAGGAGAGTGAGGGCGAGAAGGAGGGCGACGTGACA
  GCTCCCAGCGGCATCCTCGATGTCACCGTGGTCTACCTGAACCCAGAACAGCATTGCTGCCAGGAATCCA
  GTGATGAGGAGGCTTGTCCAGAGGACAAGGGACCCCAGGACCCACAGGCACTGGCGCTGGACACCCAGAT
  CCCTGCAACCCCTGGACCCAAACCCCTGGTCCGCACCAGCCGGGAGCCAGGGAAGGACGTCACGACCTCA
  GGGTACTCCTCCGTCAGCACCGCAAGTCCCACAAGCTCCGTGGACGGTGGCTTGGGGGCCCTGCCCCAAC
  CTACCTCAGTGCTGTCCCTGGACAGTGACTCGCACACACAGCCCTGCCACCATCAGGCCAGGAAGTCATG
  TTTACAGTGTCGTCCCCCAAGTCCCCCGGAGAGCAGTGTTCCCCAGCAACAGGTGAAGCGGATAAACCTA
  TGCATACACAGTGAGGAGGAGGACATGAACCTGGGCCTTGTGAGGCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204189 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSGGVVHCRCAKCFCYPTKRRIRRRPRNLTILSLPEDVLFHILKWLSVEDILAVRAVHSQLKDLVDNHA
  SVWACASFQELWPSPGNLKLFERAAEKGNFEAAVKLGIAYLYNEGLSVSDEARAEVNGLKASRFFSLAER
  LNVGAAPFIWLFIRPPWSVSGSCCKAVVHESLRAECQLQRTHKASILHCLGRVLSLFEDEEKQQQAHDLF
  EEAAHQGCLTSSYLLWESDRRTDVSDPGRCLHSFRKLRDYAAKGCWEAQLSLAKACANANQLGLEVRASS
  EIVCQLFQASQAVSKQQVFSVQKGLNDTMRYILIDWLVEVATMKDFTSLCLHLTVECVDRYLRRRLVPRY
  RLQLLGIACMVICTRFISKEILTIREAVWLTDNTYKYEDLVRMMGEIVSALEGKIRVPTVVDYKEVLLTL
  VPVELRTQHLCSFLCELSLLHTSLSAYAPARLAAAALLLARLTHGQTQPWTTQLWDLTGFSYEDLIPCVL
  SLHKKCFHDDAPKDYRQVSLTAVKQRFEDKRYGEISQEEVLSYSQLCAALGVTQDSPDPPTFLSTGEIHA
  FLSSPSGRRTKRKRENSLQEDRGSFVTTPTAELSSQEETLLGSFLDWSLDCCSGYEGDQESEGEKEGDVT
  APSGILDVTVVYLNPEQHCCQESSDEEACPEDKGPQDPQALALDTQIPATPGPKPLVRTSREPGKDVTTS
  GYSSVSTASPTSSVDGGLGALPQPTSVLSLDSDSHTQPCHHQARKSCLQCRPPSPPESSVPQQQVKRINL
  CIHSEEEDMNLGLVRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001761
• ORF Size: 2358 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001761.1, NM_001761.2, NP_001752.1, NP_001752.2
• RefSeq Size: 4287 bp
• RefSeq ORF: 2361 bp
• Locus ID: 899
• Domains: F-box, cyclin_C, CYCLIN, cyclin
• Protein Families: Druggable Genome
• MW: 87.6 kDa
• Gene Summary: This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified. [provided by RefSeq, Jul 2008]