PTP alpha (PTPRA) (NM_080841) Human Tagged ORF Clone

CAT#: RC204392

PTPRA (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, A (PTPRA), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY409009) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC223746 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified PTPRA protein (Cat# TP304392). The protein was produced from HEK293T cells transfected with PTPRA cDNA clone (Cat# RC204392) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTPRA
• Synonyms: HEPTP; HLPR; HPTPA; HPTPalpha; LRP; PTPA; PTPRL2; R-PTP-alpha; RPTPA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204392 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATTCCTGGTTCATTCTTGTTCTGCTCGGCAGTGGTCTGATATGTGTCAGTGCCAACAATGCTACCA
  CAGTTGCACCTTCTGTAGGAATTACAAGATTAATTAACTCATCAACGGCAGAACCAGTTAAAGAAGAGGC
  CAAAACTTCAAATCCAACTTCTTCACTAACTTCTCTTTCTGTGGCACCAACATTCAGCCCAAATATAACT
  CTGGGACCCACCTATTTAACCACTGTCAATTCTTCAGACTCTGACAATGGGACCACAAGAACAGCAAGCA
  CCAATTCTATAGGCATTACAATTTCACCAAATGGAACGTGGCTTCCAGATAACCAGTTCACGGATGCCAG
  AACAGAACCCTGGGAGGGGAATTCCAGCACCGCAGCAACCACTCCAGAAACTTTCCCTCCTTCAGATGAG
  ACACCAATTATTGCGGTGATGGTGGCCCTGTCCTCTCTGCTAGTGATCGTGTTTATTATCATAGTTTTGT
  ACATGTTAAGGTTTAAGAAATACAAGCAAGCTGGGAGCCATTCCAATTCTTTCCGCTTATCCAACGGCCG
  CACTGAGGATGTGGAGCCCCAGAGTGTGCCACTTCTGGCCAGATCCCCAAGCACCAACAGGAAATACCCA
  CCCCTGCCCGTGGACAAGCTGGAAGAGGAAATTAACCGGAGAATGGCAGACGACAATAAGCTCTTCAGGG
  AGGAATTCAACGCTCTCCCTGCATGTCCTATCCAGGCCACCTGTGAGGCTGCTTCCAAGGAGGAAAACAA
  GGAAAAAAATCGATATGTAAACATCTTGCCTTATGACCACTCTAGAGTCCACCTGACACCGGTTGAAGGG
  GTTCCAGATTCTGATTACATCAATGCTTCATTCATCAACGGTTACCAAGAAAAGAACAAATTCATTGCTG
  CACAAGGACCAAAAGAAGAAACGGTGAATGATTTCTGGCGGATGATCTGGGAACAAAACACAGCCACCAT
  CGTCATGGTTACCAACCTGAAGGAGAGAAAGGAGTGCAAGTGCGCCCAGTACTGGCCAGACCAAGGCTGC
  TGGACCTATGGGAATATTCGGGTGTCTGTAGAGGATGTGACTGTCCTGGTGGACTACACAGTACGGAAGT
  TCTGCATCCAGCAGGTGGGCGACATGACCAACAGAAAGCCACAGCGCCTCATCACTCAGTTCCACTTTAC
  CAGCTGGCCAGACTTTGGGGTGCCTTTTACCCCGATCGGCATGCTCAAGTTCCTCAAGAAGGTGAAGGCC
  TGTAACCCTCAGTATGCAGGGGCCATCGTGGTCCACTGCAGTGCAGGTGTAGGGCGTACAGGTACCTTTG
  TCGTCATTGATGCCATGCTGGACATGATGCATACAGAACGGAAGGTGGACGTGTATGGCTTTGTGAGCCG
  GATCCGGGCACAGCGCTGCCAGATGGTGCAAACCGATATGCAGTATGTCTTCATATACCAAGCCCTTCTG
  GAGCATTATCTCTATGGAGATACAGAACTGGAAGTGACCTCTCTAGAAACCCACCTGCAGAAAATTTACA
  ACAAAATCCCAGGGACCAGCAACAATGGATTAGAGGAGGAGTTTAAGAAGTTAACATCAATCAAAATCCA
  GAATGACAAGATGCGGACTGGAAACCTTCCAGCCAACATGAAGAAGAACCGTGTTTTACAGATCATTCCA
  TATGAATTCAACAGAGTGATCATTCCAGTTAAGCGGGGCGAAGAGAATACAGACTATGTGAACGCATCCT
  TTATTGATGGCTACCGGCAGAAGGACTCCTATATCGCCAGCCAGGGCCCTCTTCTCCACACAATTGAGGA
  CTTCTGGCGAATGATCTGGGAGTGGAAATCCTGCTCTATCGTGATGCTAACAGAACTGGAGGAGAGAGGC
  CAGGAGAAGTGTGCCCAGTACTGGCCATCTGATGGACTGGTGTCCTATGGAGATATTACAGTGGAACTGA
  AGAAGGAGGAGGAATGTGAGAGCTACACCGTCCGAGACCTCCTGGTCACCAACACCAGGGAGAATAAGAG
  CCGGCAGATCCGGCAGTTCCACTTCCATGGCTGGCCTGAAGTGGGCATCCCCAGTGACGGAAAGGGCATG
  ATCAGCATCATCGCCGCCGTGCAGAAGCAGCAGCAGCAGTCAGGGAACCACCCCATCACCGTGCACTGCA
  GCGCCGGGGCAGGAAGGACGGGGACCTTCTGTGCCCTGAGCACCGTCCTGGAGCGTGTGAAAGCAGAGGG
  GATTTTGGATGTCTTCCAGACTGTCAAGAGCCTGCGGCTACAGAGGCCACACATGGTCCAGACACTGGAA
  CAGTATGAGTTCTGCTACAAGGTGGTGCAGGAGTATATTGATGCATTCTCAGATTATGCCAACTTCAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204392 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDSWFILVLLGSGLICVSANNATTVAPSVGITRLINSSTAEPVKEEAKTSNPTSSLTSLSVAPTFSPNIT
  LGPTYLTTVNSSDSDNGTTRTASTNSIGITISPNGTWLPDNQFTDARTEPWEGNSSTAATTPETFPPSDE
  TPIIAVMVALSSLLVIVFIIIVLYMLRFKKYKQAGSHSNSFRLSNGRTEDVEPQSVPLLARSPSTNRKYP
  PLPVDKLEEEINRRMADDNKLFREEFNALPACPIQATCEAASKEENKEKNRYVNILPYDHSRVHLTPVEG
  VPDSDYINASFINGYQEKNKFIAAQGPKEETVNDFWRMIWEQNTATIVMVTNLKERKECKCAQYWPDQGC
  WTYGNIRVSVEDVTVLVDYTVRKFCIQQVGDMTNRKPQRLITQFHFTSWPDFGVPFTPIGMLKFLKKVKA
  CNPQYAGAIVVHCSAGVGRTGTFVVIDAMLDMMHTERKVDVYGFVSRIRAQRCQMVQTDMQYVFIYQALL
  EHYLYGDTELEVTSLETHLQKIYNKIPGTSNNGLEEEFKKLTSIKIQNDKMRTGNLPANMKKNRVLQIIP
  YEFNRVIIPVKRGEENTDYVNASFIDGYRQKDSYIASQGPLLHTIEDFWRMIWEWKSCSIVMLTELEERG
  QEKCAQYWPSDGLVSYGDITVELKKEEECESYTVRDLLVTNTRENKSRQIRQFHFHGWPEVGIPSDGKGM
  ISIIAAVQKQQQQSGNHPITVHCSAGAGRTGTFCALSTVLERVKAEGILDVFQTVKSLRLQRPHMVQTLE
  QYEFCYKVVQEYIDAFSDYANFK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080841
• ORF Size: 2379 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080841.1, NM_080841.2, NP_543031.1
• RefSeq Size: 3153 bp
• RefSeq ORF: 2382 bp
• Locus ID: 5786
• Cytogenetics: 20p13
• Domains: Y_phosphatase
• Protein Families: Druggable Genome, Phosphatase, Transmembrane
• MW: 89.7 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. This PTP has been shown to dephosphorylate and activate Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Three alternatively spliced variants of this gene, which encode two distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]'