Cytokeratin 16 (KRT16) (NM_005557) Human Tagged ORF Clone

CAT#: RC204470

KRT16 (Myc-DDK-tagged)-Human keratin 16 (KRT16)

Product Images

Western blot validation of overexpression lysate (Cat# LY417219) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204470 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KRT16
• Synonyms: CK16; FNEPPK; K1CP; K16; KRT16A; NEPPK; PC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204470 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCACCTGCAGCCGCCAGTTCACCTCCTCCAGCTCCATGAAGGGCTCCTGCGGCATCGGAGGCGGCA
  TCGGGGGCGGCTCCAGCCGCATCTCCTCCGTCCTGGCCGGAGGGTCCTGCCGTGCCCCCAGCACCTACGG
  GGGCGGCCTGTCTGTCTCCTCTCGCTTCTCCTCTGGGGGAGCCTGCGGGCTGGGGGGCGGCTATGGCGGT
  GGCTTCAGCAGCAGCAGCAGCTTTGGTAGTGGCTTCGGGGGAGGATATGGTGGTGGCCTTGGTGCTGGCT
  TCGGTGGTGGCTTGGGTGCTGGCTTTGGTGGTGGTTTTGCTGGTGGTGATGGGCTTCTGGTGGGCAGTGA
  GAAGGTGACCATGCAGAACCTCAATGACCGCCTGGCCTCCTACCTGGACAAGGTGCGTGCTCTGGAGGAG
  GCCAACGCCGACCTGGAAGTGAAGATCCGTGACTGGTACCAGAGGCAGCGGCCCAGTGAGATCAAAGACT
  ACAGTCCCTACTTCAAGACCATCGAGGACCTGAGGAACAAGATCATTGCGGCCACCATTGAGAATGCGCA
  GCCCATTTTGCAGATTGACAATGCCAGGCTGGCAGCCGATGACTTCAGGACCAAGTATGAGCATGAACTG
  GCCCTGCGGCAGACTGTGGAGGCCGACGTCAATGGCCTGCGCCGGGTGTTGGATGAGCTGACCCTGGCCA
  GGACTGACCTGGAGATGCAGATCGAAGGCCTGAAGGAGGAGCTGGCCTACCTGAGGAAGAACCACGAGGA
  GGAGATGCTTGCTCTGAGAGGTCAGACCGGCGGAGATGTGAACGTGGAGATGGATGCTGCACCTGGCGTG
  GACCTGAGCCGCATCCTGAATGAGATGCGTGACCAGTACGAGCAGATGGCAGAGAAAAACCGCAGAGACG
  CTGAGACCTGGTTCCTGAGCAAGACCGAGGAGCTGAACAAAGAAGTGGCCTCCAACAGCGAACTGGTACA
  GAGCAGCCGCAGTGAGGTGACGGAGCTCCGGAGGGTGCTCCAGGGCCTGGAGATTGAGCTGCAGTCCCAG
  CTCAGCATGAAAGCATCCCTGGAGAACAGCCTGGAGGAGACCAAAGGCCGCTACTGCATGCAGCTGTCCC
  AGATCCAGGGACTGATTGGCAGTGTGGAGGAGCAGCTGGCCCAGCTACGCTGTGAGATGGAGCAGCAGAG
  CCAGGAGTACCAGATCTTGCTGGATGTGAAGACGCGGCTGGAGCAGGAGATTGCCACCTACCGCCGCCTG
  CTGGAGGGCGAGGATGCCCACCTTTCCTCCCAGCAAGCATCTGGCCAATCCTATTCTTCCCGCGAGGTCT
  TCACCTCCTCCTCGTCCTCTTCGAGCCGTCAGACCCGGCCCATCCTCAAGGAGCAGAGCTCATCCAGCTT
  CAGCCAGGGCCAGAGCTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204470 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MTTCSRQFTSSSSMKGSCGIGGGIGGGSSRISSVLAGGSCRAPSTYGGGLSVSSRFSSGGACGLGGGYGG
  GFSSSSSFGSGFGGGYGGGLGAGFGGGLGAGFGGGFAGGDGLLVGSEKVTMQNLNDRLASYLDKVRALEE
  ANADLEVKIRDWYQRQRPSEIKDYSPYFKTIEDLRNKIIAATIENAQPILQIDNARLAADDFRTKYEHEL
  ALRQTVEADVNGLRRVLDELTLARTDLEMQIEGLKEELAYLRKNHEEEMLALRGQTGGDVNVEMDAAPGV
  DLSRILNEMRDQYEQMAEKNRRDAETWFLSKTEELNKEVASNSELVQSSRSEVTELRRVLQGLEIELQSQ
  LSMKASLENSLEETKGRYCMQLSQIQGLIGSVEEQLAQLRCEMEQQSQEYQILLDVKTRLEQEIATYRRL
  LEGEDAHLSSQQASGQSYSSREVFTSSSSSSSRQTRPILKEQSSSSFSQGQSS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005557
• ORF Size: 1419 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005557.2, NM_005557.3, NP_005548.2
• RefSeq Size: 1720 bp
• RefSeq ORF: 1422 bp
• Locus ID: 3868
• Cytogenetics: 17q21.2
• MW: 51.3 kDa
• Gene Summary: 'The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]'