Cytokeratin 16 (KRT16) (NM_005557) Human Untagged Clone

CAT#: SC122721

KRT16 (untagged)-Human keratin 16 (KRT16)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT16
• Synonyms: CK16; FNEPPK; K1CP; K16; KRT16A; NEPPK; PC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005557 edited
  TTTCCTTCTTTCAGCCAACTGCTCACTCGCTCACCTCCCTCCTTGGCACCATGACCACCT
  GCAGCCGCCAGTTCACCTCCTCCAGCTCCATGAAGGGCTCCTGCGGCATCGGAGGCGGCA
  TCGGGGGCGGCTCCAGCCGCATCTCCTCCGTCCTGGCCGGAGGGTCCTGCCGTGCCCCCA
  GCACCTACGGGGGCGGCCTGTCTGTCTCCTCTCGCTTCTCCTCTGGGGGAGCCTGCGGGC
  TGGGGGGCGGCTATGGCGGTGGCTTCAGCAGCAGCAGCAGCTTTGGTAGTGGCTTCGGGG
  GAGGATATGGTGGTGGCCTTGGTGCTGGCTTCGGTGGTGGCTTGGGTGCTGGCTTTGGTG
  GTGGTTTTGCTGGTGGTGATGGGCTTCTGGTGGGCAGTGAGAAGGTGACCATGCAGAACC
  TCAATGACCGCCTGGCCTCCTACCTGGACAAGGTGCGTGCTCTGGAGGAGGCCAACGCCG
  ACCTGGAAGTGAAGATCCGTGACTGGTACCAGAGGCAGCGGCCCAGTGAGATCAAAGACT
  ACAGTCCCTACTTCAAGACCATCGAGGACCTGAGGAACAAGATCATTGCGGCCACCATTG
  AGAATGCGCAGCCCATTTTGCAGATTGACAATGCCAGGCTGGCAGCCGATGACTTCAGGA
  CCAAGTATGAGCATGAACTGGCCCTGCGGCAGACTGTGGAGGCCGACGTCAATGGCCTGC
  GCCGGGTGTTGGATGAGCTGACCCTGGCCAGGACTGACCTGGAGATGCAGATCGAAGGCC
  TGAAGGAGGAGCTGGCCTACCTGAGGAAGAACCACGAGGAGGAGATGCTTGCTCTGAGAG
  GTCAGACCGGCGGAGATGTGAACGTGGAGATGGATGCTGCACCTGGCGTGGACCTGAGCC
  GCATCCTGAATGAGATGCGTGACCAGTACGAGCAGATGGCAGAGAAAAACCGCAGAGACG
  CTGAGACCTGGTTCCTGAGCAAGACCGAGGAGCTGAACAAAGAAGTGGCCTCCAACAGCG
  AACTGGTACAGAGCAGCCGCAGTGAGGTGACGGAGCTCCGGAGGGTGCTCCAGGGCCTGG
  AGATTGAGCTGCAGTCCCAGCTCAGCATGAAAGCATCCCTGGAGAACAGCCTGGAGGAGA
  CCAAAGGCCGCTACTGCATGCAGCTGTCCCAGATCCAGGGACTGATTGGCAGTGTGGAGG
  AGCAGCTGGCCCAGCTACGCTGTGAGATGGAGCAGCAGAGCCAGGAGTACCAGATCTTGC
  TGGATGTGAAGACGCGGCTGGAGCAGGAGATTGCCACCTACCGCCGCCTGCTGGAGGGCG
  AGGATGCCCACCTTTCCTCCCAGCAAGCATCTGGCCAATCCTATTCTTCCCGCGAGGTCT
  TCACCTCCTCCTCGTCCTCTTCGAGCCGTCAGACCCGGCCCATCCTCAAGGAGCAGAGCT
  CATCCAGCTTCAGCCAGGGCCAGAGCTCCTAGAACTGAGCTGCCTCTACCACAGCCTCCT
  GCCCACCAGCTGGCCTCACCTCCTGAAGGCCCGGGTCAGGACCCTGCTCTCCTGGCGCAG
  TTCCCAGCTATCTCCCCTGCTCCTCTGCTGGTGGTGGGCTAATAAAGCTGACTTTCTGGT
  TGATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005557 unedited
  CCCCGTTCAAATTTGTAATACGACTCATATAGGCGGCCGCGAATTCCCGGATTTCCTTCT
  TTCAGCCAACTGCTCACTCGCTCACCTCCCTCCTTGGCACCATGACCACCTGCAGCCGCC
  AGTTCACCTCCTCCAGCTCCATGAAGGGCTCCTGCGGCATCGGAGGCGGCATCGGGGGCG
  GCTCCAGCCGCATCTCCTCCGTCCTGGCCGGAGGGTCCTGCCGTGCCCCCAGCACCTACG
  GGGGCGGCCTGTCTGTCTCCTCTCGCTTCTCCTCTGGGGGAAGCCTGCGGGCTGGGGGGC
  GGCTATGGCGGTGGCTTCAGCAGCAGCAGCAGCTTTGGTAGTGGCTTCGGGGGAGGATAT
  GGTGGTGGCCTTGGTGCTGGCTTCGGTGGTGGCTTGGGTGCTGGCTTTGGTGGTGGTTTT
  GCTGGTGGTGATGGGCTTCTGGTGGGCAGTGAGAAGGTGACCATGCAGAACCTCAATGAC
  CGCCTGGCCTCCTACCTGGACAAGGTGCGTGCTCTGGAGGAGGCCAACGCCGACCTGGAA
  GTGAAGATCCGTGACTGGTACCAGAAGCAGCGGCCCAGTGAGATCAAAGACTACAGTCCC
  TACTTCAAGACCATCGAGGACCTGAGGAACAAGATCATTGCGGCCACCATTGAGAATGCG
  CAGCCCATTTTGCAGATTGACAATGCCAGGCTGGCAGCCGATGACTTCANGACCAAGTAT
  GAGCATGAACTGGCCCTGCGGCAGACTGTGGAGGCCGACGTCAATGGCCTGCGCNCGGTG
  TTGGATGAGCTGACCCTGGGCAAGACTGACCTGGAGATGCAAATCGAAGGCCTGAAGAGG
  AACTGGCCTACCTGAGAAGGACCCCGAGNAGGAGATGCTTGCTCTGAGAGTCAC
  
• Restriction Sites: Please inquire
• ACCN: NM_005557
• Insert Size: 1702 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005557.2, NP_005548.2
• RefSeq Size: 1688 bp
• RefSeq ORF: 1422 bp
• Locus ID: 3868
• Cytogenetics: 17q21.2
• Gene Summary: 'The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region of chromosome 17q12-q21. This keratin has been coexpressed with keratin 14 in a number of epithelial tissues, including esophagus, tongue, and hair follicles. Mutations in this gene are associated with type 1 pachyonychia congenita, non-epidermolytic palmoplantar keratoderma and unilateral palmoplantar verrucous nevus. [provided by RefSeq, Jul 2008]'