RRAS2 (NM_012250) Human Tagged ORF Clone
CAT#: RC204591
RRAS2 (Myc-DDK-tagged)-Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1
"NM_012250" in other vectors (5)
USD 415.00
USD 150.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RRAS2 |
Synonyms | NS12; TC21 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC204591 representing NM_012250.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGCGGCCGGCTGGCGGGACGGCTCCGGCCAGGAGAAGTACCGGCTCGTGGTGGTCGGCGGGGGC GGCGTGGGCAAGTCGGCGCTCACCATCCAGTTCATCCAGTCCTATTTTGTAACGGATTATGATCCAACC ATTGAAGATTCTTACACAAAGCAGTGTGTGATAGATGACAGAGCAGCCCGGCTAGATATTTTGGATACA GCAGGACAAGAAGAGTTTGGAGCCATGAGAGAACAGTATATGAGGACTGGCGAAGGCTTCCTGTTGGTC TTTTCAGTCACAGATAGAGGCAGTTTTGAAGAAATCTATAAGTTTCAAAGACAGATTCTCAGAGTAAAG GATCGTGATGAGTTCCCAATGATTTTAATTGGTAATAAAGCAGATCTGGATCATCAAAGACAGGTAACA CAGGAAGAAGGACAACAGTTAGCACGGCAGCTTAAGGTAACATACATGGAGGCATCAGCAAAGATTAGG ATGAATGTAGATCAAGCTTTCCATGAACTTGTCCGGGTTATCAGGAAATTTCAAGAGCAGGAATGTCCT CCTTCACCAGAACCAACACGGAAAGAAAAAGACAAGAAAGGCTGCCATTGTGTCATTTTC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA >Peptide sequence encoded by RC204591
Blue=ORF Red=Cloning site Green=Tag(s) MAAAGWRDGSGQEKYRLVVVGGGGVGKSALTIQFIQSYFVTDYDPTIEDSYTKQCVIDDRAARLDILDT AGQEEFGAMREQYMRTGEGFLLVFSVTDRGSFEEIYKFQRQILRVKDRDEFPMILIGNKADLDHQRQVT QEEGQQLARQLKVTYMEASAKIRMNVDQAFHELVRVIRKFQEQECPPSPEPTRKEKDKKGCHCVIF SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV Recombinant protein using RC204591 also available, TP304591M |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012250 |
ORF Size | 612 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012250.6 |
RefSeq Size | 2360 bp |
RefSeq ORF | 615 bp |
Locus ID | 22800 |
UniProt ID | P62070 |
Cytogenetics | 11p15.2 |
Domains | ras, RAN, RAS, RHO, RAB |
Protein Families | Druggable Genome |
Protein Pathways | MAPK signaling pathway, Regulation of actin cytoskeleton, Tight junction |
MW | 23.4 kDa |
Gene Summary | This gene encodes a member of the R-Ras subfamily of Ras-like small GTPases. The encoded protein associates with the plasma membrane and may function as a signal transducer. This protein may play an important role in activating signal transduction pathways that control cell proliferation. Mutations in this gene are associated with the growth of certain tumors. Pseudogenes of this gene are found on chromosomes 1 and 2. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC115460 | RRAS2 (untagged)-Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1 |
USD 310.00 |
|
SC322652 | RRAS2 (untagged)-Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1 |
USD 310.00 |
|
RG204591 | RRAS2 (GFP-tagged) - Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1 |
USD 460.00 |
|
RC204591L3 | Lenti ORF clone of Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC204591L4 | Lenti ORF clone of Human related RAS viral (r-ras) oncogene homolog 2 (RRAS2), transcript variant 1, mGFP tagged |
USD 768.00 |
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