CASQ1 (NM_001231) Human Tagged ORF Clone

CAT#: RC204696

CASQ1 (Myc-DDK-tagged)-Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein

Product Images

Western blot validation of overexpression lysate (Cat# LY420059) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204696 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified CASQ1 protein (Cat# TP304696). The protein was produced from HEK293T cells transfected with CASQ1 cDNA clone (Cat# RC204696) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CASQ1
• Synonyms: CASQ; PDIB1; VMCQA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204696 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCCAGAGCTGTGCCGGGTCTGCGGCTGGCACTGCTGTTGCTGCTGGTGCTAGGGACACCCAAGT
  CAGGGGTACAGGGGCAGGAAGGGCTGGACTTCCCTGAGTACGATGGTGTGGACCGTGTGATCAATGTCAA
  TGCAAAGAACTACAAGAATGTGTTCAAGAAGTATGAGGTGCTGGCACTCCTCTACCATGAACCCCCCGAG
  GATGACAAGGCCTCACAAAGACAATTTGAGATGGAGGAGCTGATCCTGGAGTTAGCAGCCCAAGTCCTAG
  AAGACAAGGGTGTTGGCTTCGGGCTGGTAGACTCTGAGAAGGATGCAGCTGTGGCCAAGAAACTAGGCCT
  AACTGAAGTGGACAGCATGTATGTATTCAAGGGAGATGAAGTCATTGAGTACGATGGCGAGTTTTCTGCT
  GACACCATCGTGGAGTTTCTGCTTGATGTCCTAGAGGACCCTGTGGAATTGATTGAAGGTGAACGAGAGC
  TGCAGGCGTTTGAGAATATTGAGGATGAGATCAAACTCATTGGCTACTTCAAGAGCAAAGACTCAGAGCA
  TTACAAAGCCTTCGAGGATGCAGCTGAGGAGTTTCATCCCTACATCCCCTTCTTCGCCACCTTCGACAGC
  AAGGTGGCAAAGAAGCTGACCCTGAAGCTGAATGAGATTGATTTCTACGAGGCCTTCATGGAAGAGCCTG
  TGACCATCCCAGACAAGCCCAATAGCGAAGAGGAGATTGTCAACTTCGTGGAGGAGCACAGGAGATCAAC
  CCTGAGGAAACTGAAGCCGGAGAGTATGTATGAGACCTGGGAGGATGATATGGATGGAATCCACATTGTG
  GCCTTCGCAGAGGAAGCTGATCCTGATGGTTTCGAGTTCTTAGAGACTCTCAAGGCTGTGGCCCAAGATA
  ACACTGAAAACCCAGATCTTAGCATCATCTGGATTGACCCTGATGACTTCCCCCTGCTGGTCCCATACTG
  GGAGAAGACGTTTGACATCGACTTGTCAGCCCCACAAATAGGAGTCGTCAATGTTACTGATGCGGATAGC
  GTATGGATGGAAATGGACGATGAGGAGGACCTGCCTTCTGCTGAGGAGCTGGAGGACTGGCTGGAGGATG
  TCCTGGAGGGCGAGATCAACACAGAGGACGATGACGATGATGATGATGAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204696 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGPRAVPGLRLALLLLLVLGTPKSGVQGQEGLDFPEYDGVDRVINVNAKNYKNVFKKYEVLALLYHEPPE
  DDKASQRQFEMEELILELAAQVLEDKGVGFGLVDSEKDAAVAKKLGLTEVDSMYVFKGDEVIEYDGEFSA
  DTIVEFLLDVLEDPVELIEGERELQAFENIEDEIKLIGYFKSKDSEHYKAFEDAAEEFHPYIPFFATFDS
  KVAKKLTLKLNEIDFYEAFMEEPVTIPDKPNSEEEIVNFVEEHRRSTLRKLKPESMYETWEDDMDGIHIV
  AFAEEADPDGFEFLETLKAVAQDNTENPDLSIIWIDPDDFPLLVPYWEKTFDIDLSAPQIGVVNVTDADS
  VWMEMDDEEDLPSAEELEDWLEDVLEGEINTEDDDDDDDD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001231
• ORF Size: 1170 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001231.1, NM_001231.2, NM_001231.3, NP_001222.2
• RefSeq Size: 1993 bp
• RefSeq ORF: 1191 bp
• Locus ID: 844
• Cytogenetics: 1q23.2
• Domains: Calsequestrin
• MW: 44.5 kDa
• Gene Summary: 'This gene encodes the skeletal muscle specific member of the calsequestrin protein family. Calsequestrin functions as a luminal sarcoplasmic reticulum calcium sensor in both cardiac and skeletal muscle cells. This protein, also known as calmitine, functions as a calcium regulator in the mitochondria of skeletal muscle. This protein is absent in patients with Duchenne and Becker types of muscular dystrophy. [provided by RefSeq, Jun 2013]'