CASQ1 (NM_001231) Human Untagged Clone

CAT#: SC119363

CASQ1 (untagged)-Human calsequestrin 1 (fast-twitch, skeletal muscle) (CASQ1), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CASQ1
• Synonyms: CASQ; PDIB1; VMCQA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC119363 sequence for NM_001231 edited (data generated by NextGen Sequencing)
  ATGAGTGCTACAGACAGGATGGGGCCCAGAGCTGTGCCGGGTCTGCGGCTGGCACTGCTG
  TTGCTGCTGGTGCTAGGGACACCCAAGTCAGGGGTACAGGGGCAGGAAGGGCTGGACTTC
  CCTGAGTACGATGGTGTGGACCGTGTGATCAATGTCAATGCAAAGAACTACAAGAATGTG
  TTCAAGAAGTATGAGGTGCTGGCACTCCTCTACCATGAACCCCCCGAGGATGACAAGGCC
  TCACAAAGACAATTTGAGATGGAGGAGCTGATCCTGGAGTTAGCAGCCCAAGTCCTAGAA
  GACAAGGGTGTTGGCTTCGGGCTGGTAGACTCTGAGAAGGATGCAGCTGTGGCCAAGAAA
  CTAGGCCTAACTGAAGTGGACAGCATGTATGTATTCAAGGGAGATGAAGTCATTGAGTAC
  GATGGCGAGTTTTCTGCTGACACCATCGTGGAGTTTCTGCTTGATGTCCTAGAGGACCCT
  GTGGAATTGATTGAAGGTGAACGAGAGCTGCAGGCGTTTGAGAATATTGAGGATGAGATC
  AAACTCATTGGCTACTTCAAGAGCAAAGACTCAGAGCATTACAAAGCCTTCGAGGATGCA
  GCTGAGGAGTTTCATCCCTACATCCCCTTCTTCGCCACCTTCGACAGCAAGGTGGCAAAG
  AAGCTGACCCTGAAGCTGAATGAGATTGATTTCTACGAGGCCTTCATGGAAGAGCCTGTG
  ACCATCCCAGACAAGCCCAATAGCGAAGAGGAGATTGTCAACTTCGTGGAGGAGCACAGG
  AGATCAACCCTGAGGAAACTGAAGCCGGAGAGTATGTATGAGACCTGGGAGGATGATATG
  GATGGAATCCACATTGTGGCCTTCGCAGAGGAAGCTGATCCTGATGGTTTCGAGTTCTTA
  GAGACTCTCAAGGCTGTGGCCCAAGATAACACTGAAAACCCAGATCTTAGCATCATCTGG
  ATTGACCCTGATGACTTCCCCCTGCTGGTCCCATACTGGGAGAAGACGTTTGACATCGAC
  TTGTCAGCCCCACAAATAGGAGTCGTCAATGTTACTGATGCGGATAGCGTATGGATGGAA
  ATGGACGATGAGGAGGACCTGCCTTCTGCTGAGGAGCTGGAGGACTGGCTGGAGGATGTC
  CTGGAGGGCGAGATCAACACAGAGGACGATGACGATGATGATGATGACTAG
  
  Clone variation with respect to NM_001231.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001231 unedited
  NGGTCTATCACCCGCCCGTTGNCNCAAAGGGCGGTAGGCGTGTACGGTGGGNAGGTCTAT
  ATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGG
  CCGCGAATTCGGCACCAGCTTATACCTCCCCTCCCCCAGCCCCAGCTTCATTTACCTCCG
  ACTCATCTGGTCTTATTTTTAGCTCCGGCATCTCTCCTTTTCCTTTCTTAATATGGCGAT
  GAGCTCTTAGGCCAGTGTGGGGACCGGGGCTGAGGTGCCCTGGACACTGGAGGAGGGGGA
  GGGAAGGAGCCCCTGGGAGCCTGGGGTAGAAGTGTAGGAGGTGGGAGGATTCCGGCCCGC
  ATGGAGCTGTCCTGGCCTCAGAAGGTTATCCGTCTCTCCTGCCAACCATGGAGACATATT
  TAGACAGGACCAGGTGGGGACTGAGGGGTGCCAATTTCAGGGGGCAGCTCCGGTTCCCTC
  CCCGCCCCCTGCTCCTATTCCTCCACCTGACCCTTTTTCCCTTGGCTCTGTCGGCAGTTT
  CTCCAGGACCCAGCAGTGCCCTCTGTCCACTGCTCTGGGCCATTCCCCAATCCCCCCTCC
  CACTTGAGCCCCTAACTCAGAATCTGGGACCCAGGGGCCCCTCCCTACCCCAGCTAACCT
  CTTCTGGACCAGGAGAGCCAACCCAGATCCCACTACCTCCATGAGTGCTACAGACAGGAT
  GGGGCCCAGAGCTGTGCCGGGTCTGCGGCTGGCACTGCTGTTGCTGCTGGTGCTAGGGAC
  ACCCAAGTCAGGGGTACAGGGGCAGGAAAGGCTGGACTTCCCTGAGTACGATGGTGTGGA
  CCGTGTGATCAATGTCCATGCCAAGAACTACAAGAATGTGGTCAAGAGTATGAAGGTGCT
  GGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001231
• Insert Size: 2450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001231.2, NP_001222.2
• RefSeq Size: 1884 bp
• RefSeq ORF: 1173 bp
• Locus ID: 844
• Cytogenetics: 1q23.2
• Domains: Calsequestrin
• Gene Summary: 'This gene encodes the skeletal muscle specific member of the calsequestrin protein family. Calsequestrin functions as a luminal sarcoplasmic reticulum calcium sensor in both cardiac and skeletal muscle cells. This protein, also known as calmitine, functions as a calcium regulator in the mitochondria of skeletal muscle. This protein is absent in patients with Duchenne and Becker types of muscular dystrophy. [provided by RefSeq, Jun 2013]'