RASA1 (NM_002890) Human Tagged ORF Clone

CAT#: RC205473

RASA1 (Myc-DDK-tagged)-Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RASA1 (Cat# RC205473, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASA1(Cat# TA505935). Positive lysates LY401014 (100ug) and LC401014 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY401014) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205473 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RASA1 protein (Cat# TP305473). The protein was produced from HEK293T cells transfected with RASA1 cDNA clone (Cat# RC205473) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RASA1
• Synonyms: CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP; p120RASGAP; PKWS; RASA; RASGAP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205473 representing NM_002890.
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATGGCGGCCGAGGCCGGCAGTGAGGAGGGCGGCCCGGTAACAGCCGGAGCTGGAGGAGGCGGCGCG
  GCAGCGGGCTCCAGTGCCTATCCCGCAGTGTGTCGGGTGAAGATACCCGCGGCCCTGCCTGTGGCAGCC
  GCCCCCTATCCTGGGCTGGTGGAGACCGGAGTGGCTGGAACTCTGGGTGGCGGAGCCGCTTTGGGGTCA
  GAGTTCCTAGGAGCCGGGTCTGTGGCAGGGGCACTGGGGGGAGCTGGACTGACAGGGGGAGGTACTGCT
  GCTGGCGTAGCTGGTGCTGCTGCTGGCGTGGCCGGTGCTGCTGTTGCTGGACCTAGTGGAGACATGGCT
  CTCACCAAACTGCCCACTTCGTTGCTTGCTGAGACTCTCGGGCCAGGCGGCGGTTTTCCCCCTCTGCCC
  CCTCCCCCTTACCTGCCCCCTTTGGGGGCGGGCCTCGGGACAGTGGACGAAGGTGACTCTCTGGATGGA
  CCAGAATACGAGGAGGAAGAGGTGGCCATACCGTTGACCGCTCCTCCAACTAACCAGTGGTATCACGGA
  AAACTTGACAGAACGATAGCAGAAGAACGCCTCAGGCAGGCAGGGAAGTCTGGCAGTTATCTTATAAGA
  GAGAGTGATCGGAGGCCAGGGTCCTTTGTACTTTCATTTCTTAGCCAGATGAATGTTGTCAACCATTTT
  AGGATTATTGCTATGTGTGGAGATTACTACATTGGTGGAAGACGTTTTTCTTCACTGTCAGACCTAATA
  GGTTATTACAGTCATGTTTCTTGTTTGCTTAAAGGAGAAAAATTACTTTACCCAGTTGCACCACCAGAG
  CCAGTAGAAGATAGAAGGCGTGTACGAGCTATTCTACCTTACACAAAAGTACCAGACACTGATGAAATA
  AGTTTCTTAAAAGGAGATATGTTCATTGTTCATAATGAATTAGAAGATGGATGGATGTGGGTTACAAAT
  TTAAGAACAGATGAACAAGGCCTTATTGTTGAAGACCTAGTAGAAGAGGTGGGCCGGGAAGAAGATCCA
  CATGAAGGAAAAATATGGTTCCATGGGAAGATTTCCAAACAGGAAGCTTATAATTTACTAATGACAGTT
  GGTCAAGTCTGCAGTTTTCTTGTGAGGCCCTCAGATAATACTCCTGGCGATTATTCACTTTATTTCCGG
  ACCAATGAAAATATTCAGCGATTTAAAATATGTCCAACGCCAAACAATCAGTTTATGATGGGAGGCCGG
  TATTATAACAGCATTGGGGACATCATAGATCACTATCGAAAAGAACAGATTGTTGAAGGATATTATCTT
  AAGGAACCTGTACCAATGCAGGATCAAGAACAAGTACTCAATGACACAGTGGATGGCAAGGAAATCTAT
  AATACCATCCGTCGTAAAACAAAGGATGCCTTTTATAAAAACATTGTTAAGAAAGGTTATCTTCTGAAA
  AAGGGCAAAGGAAAACGTTGGAAAAATTTATATTTTATCTTAGAGGGTAGTGATGCCCAACTTATTTAT
  TTTGAAAGCGAAAAACGAGCTACCAAACCAAAAGGATTAATAGATCTCAGTGTATGTTCTGTCTATGTC
  GTTCATGATAGTCTCTTTGGCAGGCCAAACTGTTTTCAGATAGTAGTTCAGCACTTTAGTGAAGAACAT
  TACATCTTTTACTTTGCAGGAGAAACTCCAGAACAAGCAGAGGATTGGATGAAAGGTCTGCAGGCATTT
  TGCAATTTACGGAAAAGTAGTCCAGGGACATCCAATAAACGCCTTCGTCAGGTCAGCAGCCTTGTTTTA
  CATATTGAAGAAGCCCATAAACTCCCAGTAAAACATTTTACTAATCCATATTGTAACATCTACCTGAAT
  AGTGTCCAAGTAGCAAAAACTCATGCAAGGGAAGGGCAAAACCCAGTATGGTCAGAAGAGTTTGTCTTT
  GATGATCTTCCTCCTGACATCAATAGATTTGAAATAACTCTTAGTAATAAAACAAAGAAAAGCAAAGAT
  CCTGATATCTTATTTATGCGCTGCCAGTTGAGCCGATTACAGAAAGGGCATGCCACAGATGAATGGTTT
  CTGCTCAGCTCCCATATACCATTAAAAGGTATTGAACCAGGGTCCCTGCGTGTTCGAGCACGATACTCT
  ATGGAAAAAATCATGCCAGAAGAAGAGTACAGTGAATTTAAAGAGCTTATACTGCAAAAGGAACTTCAT
  GTAGTCTATGCTTTATCACATGTATGTGGACAAGACCGAACACTACTGGCCAGCATCCTACTGAGGATT
  TTTCTTCACGAAAAGCTTGAATCGTTGTTGTTATGCACACTAAATGACAGAGAAATAAGCATGGAAGAT
  GAAGCCACTACCCTATTTCGAGCCACAACACTTGCAAGCACCTTGATGGAGCAGTATATGAAAGCCACT
  GCTACACAGTTTGTTCATCATGCTTTGAAAGACTCTATTTTAAAGATAATGGAAAGCAAGCAGTCTTGT
  GAGTTAAGTCCATCAAAGTTAGAAAAAAATGAAGATGTGAACACTAATTTAACACACCTATTGAACATA
  CTTTCAGAGCTTGTGGAGAAAATATTCATGGCTTCAGAAATACTTCCACCGACATTGAGATATATTTAT
  GGGTGTTTACAGAAATCTGTTCAGCATAAGTGGCCTACAAATACCACCATGAGAACAAGAGTTGTTAGT
  GGTTTTGTTTTTCTTCGACTCATCTGTCCTGCCATCCTGAATCCACGGATGTTCAATATCATCTCAGAT
  TCTCCATCTCCTATTGCTGCAAGAACACTGATATTAGTGGCTAAATCTGTGCAGAACTTAGCAAATCTT
  GTGGAATTTGGAGCTAAGGAGCCCTACATGGAAGGTGTCAATCCATTCATCAAAAGCAACAAACATCGT
  ATGATCATGTTTTTAGATGAACTTGGGAATGTACCTGAACTTCCGGACACTACAGAGCATTCTAGAACG
  GACCTGTCCCGTGATTTAGCAGCATTGCATGAGATTTGCGTGGCTCATTCAGATGAACTTCGAACGCTC
  AGTAATGAGCGTGGTGCACAGCAGCACGTATTGAAAAAGCTTCTGGCTATAACAGAACTGCTTCAACAA
  AAACAAAACCAGTATACAAAAACCAATGATGTCAGG
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
  Protein Sequence

  >Peptide sequence encoded by RC205473
  Blue=ORF Red=Cloning site Green=Tag(s)
  
  MMAAEAGSEEGGPVTAGAGGGGAAAGSSAYPAVCRVKIPAALPVAAAPYPGLVETGVAGTLGGGAALGS
  EFLGAGSVAGALGGAGLTGGGTAAGVAGAAAGVAGAAVAGPSGDMALTKLPTSLLAETLGPGGGFPPLP
  PPPYLPPLGAGLGTVDEGDSLDGPEYEEEEVAIPLTAPPTNQWYHGKLDRTIAEERLRQAGKSGSYLIR
  ESDRRPGSFVLSFLSQMNVVNHFRIIAMCGDYYIGGRRFSSLSDLIGYYSHVSCLLKGEKLLYPVAPPE
  PVEDRRRVRAILPYTKVPDTDEISFLKGDMFIVHNELEDGWMWVTNLRTDEQGLIVEDLVEEVGREEDP
  HEGKIWFHGKISKQEAYNLLMTVGQVCSFLVRPSDNTPGDYSLYFRTNENIQRFKICPTPNNQFMMGGR
  YYNSIGDIIDHYRKEQIVEGYYLKEPVPMQDQEQVLNDTVDGKEIYNTIRRKTKDAFYKNIVKKGYLLK
  KGKGKRWKNLYFILEGSDAQLIYFESEKRATKPKGLIDLSVCSVYVVHDSLFGRPNCFQIVVQHFSEEH
  YIFYFAGETPEQAEDWMKGLQAFCNLRKSSPGTSNKRLRQVSSLVLHIEEAHKLPVKHFTNPYCNIYLN
  SVQVAKTHAREGQNPVWSEEFVFDDLPPDINRFEITLSNKTKKSKDPDILFMRCQLSRLQKGHATDEWF
  LLSSHIPLKGIEPGSLRVRARYSMEKIMPEEEYSEFKELILQKELHVVYALSHVCGQDRTLLASILLRI
  FLHEKLESLLLCTLNDREISMEDEATTLFRATTLASTLMEQYMKATATQFVHHALKDSILKIMESKQSC
  ELSPSKLEKNEDVNTNLTHLLNILSELVEKIFMASEILPPTLRYIYGCLQKSVQHKWPTNTTMRTRVVS
  GFVFLRLICPAILNPRMFNIISDSPSPIAARTLILVAKSVQNLANLVEFGAKEPYMEGVNPFIKSNKHR
  MIMFLDELGNVPELPDTTEHSRTDLSRDLAALHEICVAHSDELRTLSNERGAQQHVLKKLLAITELLQQ
  KQNQYTKTNDVR
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
  
  Recombinant protein using RC205473 also available, TP305473M
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002890
• ORF Size: 3141 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002890.3
• RefSeq Size: 4402 bp
• RefSeq ORF: 3144 bp
• Locus ID: 5921
• UniProt ID: P20936
• Cytogenetics: 5q14.3
• Domains: C2, SH2, SH3, PH, RasGAP
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, MAPK signaling pathway
• MW: 116.4 kDa
• Gene Summary: The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]