RASA1 (NM_002890) Human Untagged Clone

CAT#: SC118332

RASA1 (untagged)-Human RAS p21 protein activator (GTPase activating protein) 1 (RASA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RASA1
• Synonyms: CM-AVM; CMAVM; CMAVM1; GAP; p120; p120GAP; p120RASGAP; PKWS; RASA; RASGAP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118332 sequence for NM_002890 edited (data generated by NextGen Sequencing)
  ATGATGGCGGCCGAGGCCGGCAGTGAGGAGGGCGGCCCGGTAACAGCCGGAGCTGGAGGA
  GGCGGCGCGGCAGCGGGCTCCAGTGCCTATCCCGCAGTGTGTCGGGTGAAGATACCCGCG
  GCCCTGCCTGTGGCAGCCGCCCCCTATCCTGGGCTGGTGGAGACCGGAGTGGCTGGAACT
  CTGGGTGGCGGAGCCGCTTTGGGGTCAGAGTTCCTAGGAGCCGGGTCTGTGGCAGGGGCA
  CTGGGGGGAGCTGGACTGACAGGGGGAGGTACTGCTGCTGGCGTAGCTGGTGCTGCTGCT
  GGCGTGGCCGGTGCTGCTGTTGCTGGACCTAGTGGAGACATGGCTCTCACCAAACTGCCC
  ACTTCGTTGCTTGCTGAGACTCTCGGGCCAGGCGGCGGTTTTCCCCCTCTGCCCCCTCCC
  CCTTACCTGCCCCCTTTGGGGGCGGGCCTCGGGACAGTGGACGAAGGTGACTCTCTGGAT
  GGACCAGAATACGAGGAGGAAGAGGTGGCCATACCGTTGACCGCTCCTCCAACTAACCAG
  TGGTATCACGGAAAACTTGACAGAACGATAGCAGAAGAACGCCTCAGGCAGGCAGGGAAG
  TCTGGCAGTTATCTTATAAGAGAGAGTGATCGGAGGCCAGGGTCCTTTGTACTTTCATTT
  CTTAGCCAGATGAATGTTGTCAACCATTTTAGGATTATTGCTATGTGTGGAGATTACTAC
  ATTGGTGGAAGACGTTTTTCTTCACTGTCAGACCTAATAGGTTATTACAGTCATGTTTCT
  TGTTTGCTTAAAGGAGAAAAATTACTTTACCCAGTTGCACCACCAGAGCCAGTAGAAGAT
  AGAAGGCGTGTACGAGCTATTCTACCTTACACAAAAGTACCAGACACTGATGAAATAAGT
  TTCTTAAAAGGAGATATGTTCATTGTTCATAATGAATTAGAAGATGGATGGATGTGGGTT
  ACAAATTTAAGAACAGATGAACAAGGCCTTATTGTTGAAGACCTAGTAGAAGAGGTGGGC
  CGGGAAGAAGATCCACATGAAGGAAAAATATGGTTCCATGGGAAGATTTCCAAACAGGAA
  GCTTATAATTTACTAATGACAGTTGGTCAAGTCTGCAGTTTTCTTGTGAGGCCCTCAGAT
  AATACTCCTGGCGATTATTCACTTTATTTCCGGACCAATGAAAATATTCAGCGATTTAAA
  ATATGTCCAACGCCAAACAATCAGTTTATGATGGGAGGCCGGTATTATAACAGCATTGGG
  GACATCATAGATCACTATCGAAAAGAACAGATTGTTGAAGGATATTATCTTAAGGAACCT
  GTACCAATGCAGGATCAAGAACAAGTACTCAATGACACAGTGGATGGCAAGGAAATCTAT
  AATACCATCCGTCGTAAAACAAAGGATGCCTTTTATAAAAACATTGTTAAGAAAGGTTAT
  CTTCTGAAAAAGGGCAAAGGAAAACGTTGGAAAAATTTATATTTTATCTTAGAGGGTAGT
  GATGCCCAACTTATTTATTTTGAAAGCGAAAAACGAGCTACCAAACCAAAAGGATTAATA
  GATCTCAGTGTATGTTCTGTCTATGTCGTTCATGATAGTCTCTTTGGCAGGCCAAACTGT
  TTTCAGATAGTAGTTCAGCACTTTAGTGAAGAACATTACATCTTTTACTTTGCAGGAGAA
  ACTCCAGAACAAGCAGAGGATTGGATGAAAGGTCTGCAGGCATTTTGCAATTTACGGAAA
  AGTAGTCCAGGGACATCCAATAAACGCCTTCGTCAGGTCAGCAGCCTTGTTTTACATATT
  GAAGAAGCCCATAAACTCCCAGTAAAACATTTTACTAATCCATATTGTAACATCTACCTG
  AATAGTGTCCAAGTAGCAAAAACTCATGCAAGGGAAGGGCAAAACCCAGTATGGTCAGAA
  GAGTTTGTCTTTGATGATCTTCCTCCTGACATCAATAGATTTGAAATAACTCTTAGTAAT
  AAAACAAAGAAAAGCAAAGATCCTGATATCTTATTTATGCGCTGCCAGTTGAGCCGATTA
  CAGAAAGGGCATGCCACAGATGAATGGTTTCTGCTCAGCTCCCATATACCATTAAAAGGT
  ATTGAACCAGGGTCCCTGCGTGTTCGAGCACGATACTCTATGGAAAAAATCATGCCAGAA
  GAAGAGTACAGTGAATTTAAAGAGCTTATACTGCAAAAGGAACTTCATGTAGTCTATGCT
  TTATCACATGTATGTGGACAAGACCGAACACTACTGGCCAGCATCCTACTGAGGATTTTT
  CTTCACGAAAAGCTTGAATCGTTGTTGTTATGCACACTAAATGACAGAGAAATAAGCATG
  GAAGATGAAGCCACTACCCTATTTCGAGCCACAACACTTGCAAGCACCTTGATGGAGCAG
  TATATGAAAGCCACTGCTACACAGTTTGTTCATCATGCTTTGAAAGACTCTATTTTAAAG
  ATAATGGAAAGCAAGCAGTCTTGTGAGTTAAGTCCATCAAAGTTAGAAAAAAATGAAGAT
  GTGAACACTAATTTAACACACCTATTGAACATACTTTCAGAGCTTGTGGAGAAAATATTC
  ATGGCTTCAGAAATACTTCCACCGACATTGAGATATATTTATGGGTGTTTACAGAAATCT
  GTTCAGCATAAGTGGCCTACAAATACCACCATGAGAACAAGAGTTGTTAGTGGTTTTGTT
  TTTCTTCGACTCATCTGTCCTGCCATCCTGAATCCACGGATGTTCAATATCATCTCAGAT
  TCTCCATCTCCTATTGCTGCAAGAACACTGATATTAGTGGCTAAATCTGTGCAGAACTTA
  GCAAATCTTGTGGAATTTGGAGCTAAGGAGCCCTACATGGAAGGTGTCAATCCATTCATC
  AAAAGCAACAAACATCGTATGATCATGTTTTTAGATGAACTTGGGAATGTACCTGAACTT
  CCGGACACTACAGAGCATTCTAGAACGGACCTGTCCCGTGATTTAGCAGCATTGCATGAG
  ATTTGCGTGGCTCATTCAGATGAACTTCGAACGCTCAGTAATGAGCGTGGTGCACAGCAG
  CACGTATTGAAAAAGCTTCTGGCTATAACAGAACTGCTTCAACAAAAACAAAACCAGTAT
  ACAAAAACCAATGATGTCAGGTAG
  
  Clone variation with respect to NM_002890.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002890 unedited
  CAGAATTTTGTAATACGACTCACTATAGGGGCGGCCGCGAATTCGCACGAGGGGCGCGGC
  GGCGGGCTCTCTCCTTTTGTTGTTGTTTCCTCAGCCTGGGGAGCTGAAGGGGAGACGCGT
  CTGGGTGGGGCTGCTCGGAGCCCGGGCCTGGTGGCCCCTGGGGCTCCCGGGCGGGCAGGG
  TAGGGCAGAGTAGAGCGGGCTTCAACATGATGGCGGCCGAGGCCGGCAGTGAGGAGGGCG
  GCCCGGTAACAGCCGGAGCTGGAGGAGGCGGCGCGGCAGCGGGCTCCAGTGCCTATCCCG
  CAGTGTGTCGGGTGAAGATACCCGCGGCCCTGCCTGTGGCAGCCGCCCCCTATCCTGGGC
  TGGTGGAGACCGGAGTGGCTGGAACTCTGGGTGGCGGAGCCGCTTTGGGGTCAGAGTTCC
  TAGGAGCCGGGTCTGTGGCAGGGGCACTGGGGGGAGCTGGACTGACAGGGGGAGGTACTG
  CTGCTGGCGTAGCTGGTGCTGCTGCTGGCGTGGCCGGTGCTGCTGTTGCTGGACCTAGTG
  GAGACATGGCTCTCACCAAACTGCCCACTTCGTTGCTTGCTGAGACTCTCGGGCCAGGCG
  GCGGTTTTCCCCCTCTGCCCCCTCCCCCTTACCTGCCCCCTTTGGGGGCGGGCCTCGGGA
  CAGTGGACGAAGGTGACTCTCTGGATGGACCAGAATACGAGGAGGAAGAGGTGGCCATAC
  CGTTGACCGCTCCTCCAACTAACCAGTGGTATCACGGAAAACTTGACAGAACGATAGCAG
  AAGAACGCCTCANGCAGGCAGGGAAGTCTGGCAGTTATCTTATAAGAGAGAGTGATCGGG
  AGCCAGGGTCCTTTGTACTTTCATTTCTAGCCAGATGAATGGTGTCAACCATTTTAGGAT
  ATTGCTATGTGTGGAGATACTACATGGGTGGAAGACGNTNTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002890 unedited
  GCTATGGACCGGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCAAGATAT
  CCCTTGGTTTTATTTTAACTTAACCTTTGTGAATACTTCACAAAAATACACATACAGAAG
  TAAATAAAACTATACAGTTGAACTAAATATCCAGCAGATACAACAAGTAGTCAGAAGTTC
  AAAGATGGATAAGTTAGGCAGCTGATGAGGTTGTCTGCATAATCAAAGGGGTAGCACAAA
  TAATGAACAAATAACTTTTAACAAAAGCATTCTAGTTAACATAAAAGAAATTGGTAGTCT
  ATAAAATGGTTGAATGGGTAACATGCATTGATCAATACAACAACCAATAATATTTACTGA
  ATTCAAACTATTTACTTGTAAACATTTAACAAAGCATTATGAGATCTAAATACAGTCTTT
  GACAATTTCAACAAAATAAGTCGTATGTTTAAAAAATAAAGCTGATGAGCAATAGATCAT
  TCTATATATTTTCTTTCAACAGTTCCAGCAAGAGAAATAGCAGAGTATTTTTTAAAAGTA
  TACAGCAAAGATTGTTCCTATTATAATGACAAACTCTGGAAACTGTAAATACAAATAATC
  ATTGAACAGTCAAATGGTCATATTCCTAATCAGGAAGTAGTTACAGTAAGAACAGTCTGC
  AATCCAGTTATACACAATTGTAATAAGAATGTGTTTCTTGTCAGNTGATTTCATAGTTCT
  TTCTCTAAGAGACAGCAAACTTGGCACTGGGTTGACTGAAATATATTTTGAAGGTCGAGA
  ATCCAGAACAGTCTAGCATTTCGTTTGGGAAAGGGGCCTGACACCAGCTAAAAGGTGTTA
  AGATGAGTCCTGGGGAATAGGAAATTTCTGCCAGAAGCTACAAGGTGCTCCAAGTTATAC
  CACCAATTTGGGATTTGTTGCAAGTCACTCCTGATGGGGGAAAGGGCTTTTTGGGAGACA
  GGGAATAAC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002890
• Insert Size: 4230 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002890.1, NP_002881.1
• RefSeq Size: 4307 bp
• RefSeq ORF: 3144 bp
• Locus ID: 5921
• Cytogenetics: 5q14.3
• Domains: C2, SH2, SH3, PH, RasGAP
• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is located in the cytoplasm and is part of the GAP1 family of GTPase-activating proteins. The gene product stimulates the GTPase activity of normal RAS p21 but not its oncogenic counterpart. Acting as a suppressor of RAS function, the protein enhances the weak intrinsic GTPase activity of RAS proteins resulting in the inactive GDP-bound form of RAS, thereby allowing control of cellular proliferation and differentiation. Mutations leading to changes in the binding sites of either protein are associated with basal cell carcinomas. Mutations also have been associated with hereditary capillary malformations (CM) with or without arteriovenous malformations (AVM) and Parkes Weber syndrome. Alternative splicing results in two isoforms where the shorter isoform, lacking the N-terminal hydrophobic region but retaining the same activity, appears to be abundantly expressed in placental but not adult tissues. [provided by RefSeq, May 2012]'
  Transcript Variant: This variant (1) lacks the alternatively spliced insert found in variant 2, resulting in translation of the longer isoform (1).