PEA15 (NM_003768) Human Tagged ORF Clone
CAT#: RC205507
PEA15 (Myc-DDK-tagged)-Human phosphoprotein enriched in astrocytes 15 (PEA15)
"NM_003768" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PEA15 |
Synonyms | HMAT1; HUMMAT1H; MAT1; MAT1H; PEA-15; PED; PED-PEA15; PED/PEA15 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC205507 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGAGTACGGGACCCTCCTGCAAGACCTGACCAACAACATCACCCTTGAAGATCTAGAACAGCTCA AGTCGGCCTGCAAGGAAGACATCCCCAGCGAAAAGAGTGAGGAGATCACTACTGGCAGTGCCTGGTTTAG CTTCCTGGAGAGCCACAACAAGCTGGACAAAGACAACCTCTCCTACATTGAGCACATCTTTGAGATCTCC CGCCGTCCTGACCTACTCACTATGGTGGTTGACTACAGAACCCGTGTGCTGAAAATCTCTGAGGAGGATG AGCTGGACACCAAGCTAACCCGTATCCCCAGTGCCAAGAAGTACAAAGACATTATCCGGCAGCCCTCTGA GGAAGAGATCATCAAATTGGCTCCCCCACCGAAGAAGGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC205507 protein sequence
Red=Cloning site Green=Tags(s) MAEYGTLLQDLTNNITLEDLEQLKSACKEDIPSEKSEEITTGSAWFSFLESHNKLDKDNLSYIEHIFEIS RRPDLLTMVVDYRTRVLKISEEDELDTKLTRIPSAKKYKDIIRQPSEEEIIKLAPPPKKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_003768 |
ORF Size | 390 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003768.1, NM_003768.2, NM_003768.3, NM_003768.4, NP_003759.1 |
RefSeq Size | 2509 bp |
RefSeq ORF | 393 bp |
Locus ID | 8682 |
Cytogenetics | 1q23.2 |
Domains | DED |
Protein Families | Druggable Genome |
MW | 15 kDa |
Gene Summary | This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108132 | PEA15 (untagged)-Human phosphoprotein enriched in astrocytes 15 (PEA15) |
USD 310.00 |
|
RG205507 | PEA15 (GFP-tagged) - Human phosphoprotein enriched in astrocytes 15 (PEA15) |
USD 460.00 |
|
RC205507L1 | Lenti ORF clone of Human phosphoprotein enriched in astrocytes 15 (PEA15), Myc-DDK-tagged |
USD 768.00 |
|
RC205507L2 | Lenti ORF clone of Human phosphoprotein enriched in astrocytes 15 (PEA15), mGFP tagged |
USD 620.00 |
|
RC205507L3 | Lenti ORF clone of Human phosphoprotein enriched in astrocytes 15 (PEA15), Myc-DDK-tagged |
USD 620.00 |
|
RC205507L4 | Lenti ORF clone of Human phosphoprotein enriched in astrocytes 15 (PEA15), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review