Stromal interaction molecule 1 (STIM1) (NM_003156) Human Tagged ORF Clone
CAT#: RC205678L3
- LentiORF®
Lenti ORF clone of Human stromal interaction molecule 1 (STIM1), Myc-DDK-tagged
"NM_003156" in other vectors (6)
Product Images
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | STIM1 |
Synonyms | D11S4896E; GOK; IMD10; STRMK; TAM; TAM1 |
Vector | pLenti-C-Myc-DDK-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC205678).
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Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_003156 |
ORF Size | 2055 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_003156.2, NP_003147.2 |
RefSeq Size | 4039 bp |
RefSeq ORF | 2058 bp |
Locus ID | 6786 |
Cytogenetics | 11p15.4 |
Protein Families | Transmembrane |
MW | 77.42 kDa |
Gene Summary | 'This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC118136 | STIM1 (untagged)-Human stromal interaction molecule 1 (STIM1) |
USD 310.00 |
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RC205678 | STIM1 (Myc-DDK-tagged)-Human stromal interaction molecule 1 (STIM1) |
USD 610.00 |
|
RG205678 | STIM1 (GFP-tagged) - Human stromal interaction molecule 1 (STIM1) |
USD 670.00 |
|
RC205678L1 | Lenti ORF clone of Human stromal interaction molecule 1 (STIM1), Myc-DDK-tagged |
USD 1,008.00 |
|
RC205678L2 | Lenti ORF clone of Human stromal interaction molecule 1 (STIM1), mGFP tagged |
USD 810.00 |
|
RC205678L4 | Lenti ORF clone of Human stromal interaction molecule 1 (STIM1), mGFP tagged |
USD 1,008.00 |
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