Stromal interaction molecule 1 (STIM1) (NM_003156) Human Untagged Clone

CAT#: SC118136

STIM1 (untagged)-Human stromal interaction molecule 1 (STIM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STIM1
• Synonyms: D11S4896E; GOK; IMD10; STRMK; TAM; TAM1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003156 edited
  GAATTCGGCACGAGGCTTGCCTGGAGACCGTCGGCTGCACTCCCGGGCTCCTGGCTTTGCCTCTGGGATC
  CCGAGGTGTCCACATCAGACGCATGTTGACTGAGACCTAGAGTCATGGATGTATGCGTCCGTCTTGCCCT
  GTGGCTCCTCTGGGGACTCCTCCTGCACCAGGGCCAGAGCCTCAGCCATAGTCACAGTGAGAAGGCGACA
  GGAACCAGCTCGGGGGCCAACTCTGAGGAGTCCACTGCAGCAGAGTTTTGCCGAATTGACAAGCCCCTGT
  GTCACAGTGAGGATGAGAAACTCAGCTTCGAGGCAGTCCGTAACATCCACAAACTGATGGACGATGATGC
  CAATGGTGATGTGGATGTGGAAGAAAGTGATGAGTTCCTGAGGGAAGACCTCAATTACCATGACCCAACA
  GTGAAACACAGCACCTTCCATGGTGAGGATAAGCTCATCAGCGTGGAGGACCTGTGGAAGGCATGGAAGT
  CATCAGAAGTATACAATTGGACCGTGGATGAGGTGGTACAGTGGCTGATCACATATGTGGAGCTGCCTCA
  GTATGAGGAGACCTTCCGGAAGCTGCAGCTCAGTGGCCATGCCATGCCAAGGCTGGCTGTCACCAACACC
  ACCATGACAGGGACTGTGCTGAAGATGACAGACCGGAGTCATCGGCAGAAGCTGCAGCTGAAGGCTCTGG
  ATACAGTGCTCTTTGGGCCTCCTCTCTTGACTCGCCATAATCACCTCAAGGACTTCATGCTGGTGGTGTC
  TATCGTTATTGGTGTGGGCGGCTGCTGGTTTGCCTATATCCAGAACCGTTACTCCAAGGAGCACATGAAG
  AAGATGATGAAGGACTTGGAGGGGTTACACCGAGCTGAGCAGAGTCTGCATGACCTTCAGGAAAGGCTGC
  ACAAGGCCCAGGAGGAGCACCGCACAGTGGAGGTGGAGAAGGTCCATCTGGAAAAGAAGCTGCGCGATGA
  GATCAACCTTGCTAAGCAGGAAGCCCAGCGGCTGAAGGAGCTGCGGGAGGGTACTGAGAATGAGCGGAGC
  CGCCAAAAATATGCTGAGGAGGAGTTGGAGCAGGTTCGGGAGGCCTTGAGGAAAGCAGAGAAGGAGCTAG
  AATCTCACAGCTCATGGTATGCTCCAGAGGCCCTTCAGAAGTGGCTGCAGCTGACACATGAGGTGGAGGT
  GCAATATTACAACATCAAGAAGCAAAATGCTGAGAAGCAGCTGCTGGTGGCCAAGGAGGGGGCTGAGAAG
  ATAAAAAAGAAGAGAAACACACTCTTTGGCACCTTCCACGTGGCCCACAGCTCTTCCCTGGATGATGTAG
  ATCATAAAATTCTAACAGCTAAGCAAGCACTGAGCGAGGTGACAGCAGCATTGCGGGAGCGCCTGCACCG
  CTGGCAACAGATCGAGATCCTCTGTGGCTTCCAGATTGTCAACAACCCTGGCATCCACTCACTGGTGGCT
  GCCCTCAACATAGACCCCAGCTGGATGGGCAGTACACGCCCCAACCCTGCTCACTTCATCATGACTGACG
  ACGTGGATGACATGGATGAGGAGATTGTGTCTCCCTTGTCCATGCAGTCCCCTAGCCTGCAGAGCAGTGT
  TCGGCAGCGCCTGACGGAGCCACAGCATGGCCTGGGATCTCAGAGGGATTTGACCCATTCCGATTCGGAG
  TCCTCCCTCCACATGAGTGACCGCCAGCGTGTGGCCCCCAAACCTCCTCAGATGAGCCGTGCTGCAGACG
  AGGCTCTCAATGCCATGACTTCCAATGGCAGCCACCGGCTGATCGAGGGGGTCCACCCAGGGTCTCTGGT
  GGAGAAACTGCCTGACAGCCCTGCCCTGGCCAAGAAGGCATTACTGGCGCTGAACCATGGGCTGGACAAG
  GCCCACAGCCTGATGGAGCTGAGCCCCTCAGCCCCACCTGGTGGCTCTCCACATTTGGATTCTTCCCGTT
  CTCACAGCCCCAGCTCCCCAGACCCAGACACACCATCTCCAGTTGGGGACAGCCGAGCCCTGCAAGCCAG
  CCGAAACACACGCATTCCCCACCTGGCTGGCAAGAAGGCTGTGGCTGAGGAGGATAATGGCTCTATTGGC
  GAGGAAACAGACTCCAGCCCAGGCCGGAAGAAGTTTCCCCTCAAAATCTTTAAGAAGCCTCTTAAGAAGT
  AGGCAGGATGGGGTGGCAGTAAAGGGACAGCTTGTCCTTCCCTGGGTGTTCTGTCTCTCCTTCCCTCCCT
  TCCTTCAAGATAACTGGCCCCAAGAGTGGGGCATGGGAAGGGCTGGTCCAGGGGTCTGGGCACTGTACAT
  ACCTGCCCCCTCATCCTTGGGTCCTTCATTATTATTTATTAACTGACCACCATGGCCTGCCTGCCCTGCC
  TCCGTCCCAACCATGGGCTGCTGCTGTCACTCCCTCTCCACTTCAGTGCATGTCTTAGTTGCTGTTCCCT
  CAGCTCCCAGCTCCACCTCTGGGGTTCAGCTTCTGTCTCTGCTGTCCCAGTTTTGAGGTTTGGTTTCTTG
  TTTCTGTCTCTTGCTTTCGGGCTCCTCCCTCCCACCACTCCCCAACTTCCCCTAGCAGTTGCAGGGAAGA
  TAGGACGAGTAGCTTCTGACATGTGTGCCTCAGATCTGTTCCACCCCACTCACAGTGGTTCTGTTTGCTC
  CAGACTGGGGCTAGGGCCTAATCTTTGAAGTTTGTTCTTTGGTATTGATGTGGGTCAGAAGGAGCCTCAT
  CCTAATCTCACTCAGGCCTCCAGGGATCCATGGGGGAGTGAAACCAATTCTCAGAGAACAACCCACCAGA
  GACTTTTAAAGAGAGGCCAGGCTTGGGAATGGGTTGGGAGAGGCATCTGTTCATTGGAGCATGAGTGGAT
  GCCAGAACTGTAGGTTATAAGGCAGTCACTTTTTCTCTCTACTCCCACCCACACCTGCCTCCCTCTTACC
  CCTGCTCCCCCACACTGCAGGAGGATTTGTCTCTAAGAGGTGCTGCCCCAAAGCTCCCCAAGCATCAATA
  CTCCTAGGGCTCAGGACAAGTGGCTCCCCTGGCCAGGAGAGCCACAGCCATGATACAGGGCTCTTATGGA
  GCCCTGGAGTTGTTGGGCAAGGATGCTGTCATTTTTTGAACCAAAAGACAAACAGGTTAAAAGGAAAAAA
  AGTAATCTGAATTTCCCAAGTGCCTACGCTGCATATTCCCCTTGTTAGATCCCATTTTCATGTTACTTTG
  TAGCCTTGGCCAGAGGCTCAAAAAGGACACAACCAGTTTGGGGAAGGGGTGGCTAAGGAAGATGGTATAG
  GTGAAGGCGGCTGTGTGACCACTTTCCCCCACCCTTCCCACCCTCTAGACAACTCTCTCCCTTACCTGTT
  TTTGCTATGGCTGTAAAGGTATTTTTCCTCTGCCCCACTCCCTGCCATACCTTTATCCTGGGATCCTATT
  TTGGGCCTGGGGTGGGTATACCTGGGGCTGGTCTTAGGAGGGTGCTAGGCTGCAGACTGCCTTGTACTCC
  CTGGACACCCTCAAATGGGGTTTTCTGTGTTATTTCATAAAATTCTTTGAAGTCCAATAAAGCATGTAGG
  AGATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003156 unedited
  NGGGGGTTCACATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCTTGC
  CTGGAGACCGTCGGCTGCACTCCCGGGCTCCTGGCTTTGCCTCTGGGATCCCGAGGTGTC
  CACATCAGACGCATGTTGACTGAGACCTAGAGTCATGGATGTATGCGTCCGTCTTGCCCT
  GTGGCTCCTCTGGGGACTCCTCCTGCACCAGGGCCAGAGCCTCAGCCATAGTCACAGTGA
  GAAGGCGACAGGAACCAGCTCGGGGGCCAACTCTGAGGAGTCCACTGCAGCAGAGTTTTG
  CCGAATTGACAAGCCCCTGTGTCACAGTGAGGATGAGAAACTCAGCTTCGAGGCAGTCCG
  TAACATCCACAAACTGATGGACGATGATGCCAATGGTGATGTGGATGTGGAAGAAAGTGA
  TGAGTTCCTGAGGGAAGACCTCAATTACCATGACCCAACAGTGAAACACAGCACCTTCCA
  TGGTGAGGATAAGCTCATCAGCGTGGAGGACCTGTGGAAGGCATGGAAGTCATCAGAAGT
  ATACAATTGGACCGTGGATGAGGTGGTACAGTGGCTGATCACATATGTGGAGCTGCCTCA
  GTATGAGGAGACCTTCCGGAAGCTGCAGCTCAGTGGCCATGCCATGCCAAGGCTGGCTGT
  CACCAACACCACCATGACAGGGACTGTGCTGAAGATGACAGACCGGAGTCATCGGCAGAA
  GCTGCAGCTGANAGCTCTGGATACAGTGCTCTNTGGGCCTNCTCTCTTGACTCGCCATAA
  TCACCTCAAGGACTTCATGCTGGNNTGGTGTCTATCGTTATTGGGTGTGGGCCGCTGCTT
  GGTTTGCCTATATCCAGAACCGTTACTNCCAGGAGCACATGAANAAAATGATGAAGGACT
  TTGGAGGGNTACACCGAGCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003156 unedited
  ACTATGGAACCGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTAAAATCTCCTACATGCTTTATTGGACTTCAAAAAATTTTATGAAATAACACAGAAAA
  ACCCCATTTGAGGGTGTCCAGGGAGTACAAGGCAGTCTGCAGCCTAGCACCCTCCTAAGA
  CCAGCCCCAGGTATACCCACCCCAGGCCCAAAATAGGATCCCAGGATAAAGGTATGGCAG
  GGAGTGGGGCAGAGGAAAAATACCTTTACAGCCATAGCAAAAACAGGTAAGGGAGAGAGT
  TGTCTAAAGGGTGGGAAGGGTGGGGGAAAGTGGTCACACAGCCGCCTTCACCTATACCAT
  CTTCCTTAGCCACCCCTTCCCCAAACTGGTTGTGTCCTTTTTGAGCCTCTGGCCAAGGCT
  ACAAAGTAACATGAAAATGGGATCTAACAAGGGGAATATGCAGCGTAGGCACTTGGGAAA
  TTCAGATTACTTTTTTTCCTTTTAACCTGTTTGTCTTTTGGTTCAAAAAATGACAGCATC
  CTTGCCCAACAACTCCAGGGCTCCATAAGAGCCCTGTATCATGGCTGTGGCTCTCCTGGC
  CAGGGGAGCCACTTGTCCTGAGCCCTAGGAGTATTGATGCTTGGGGAGCTTTGGGGCAGC
  ACCTCTTAGAGAAAATCCTCCTGCAGTGTGGGGGAGCAGGGGTAANAAGGAGGCAGGTGT
  GGGTGGGAGTANAAAGAAAAAGTGACTGCCTTATAACCTACAGTTCTGGCATCCACTCAT
  GCTCCAATGAACAAGATGCCTCTCCCAACCCATTTCCAAGCCTGGCCTTTCTTTAAAGTC
  TCTGGTGGGGTGTTCTCTGAAAAATGGTTTCACCTCCCCATGGATCCCTGGAAGGCTGAA
  TGAAAATAAGAGGAGGCTCCTTCTGACCCCATCAATCCAAAGACAAACTTCAAAAATAGG
  CCCTAGCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003156
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003156.2, NP_003147.2
• RefSeq Size: 4039 bp
• RefSeq ORF: 2058 bp
• Locus ID: 6786
• Cytogenetics: 11p15.4
• Protein Families: Transmembrane
• Gene Summary: 'This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]'
  Transcript Variant: This variant (2, also known as STIM1S) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.