FBXL2 (NM_012157) Human Tagged ORF Clone

CAT#: RC206390

FBXL2 (Myc-DDK-tagged)-Human F-box and leucine-rich repeat protein 2 (FBXL2), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY415925) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206390 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified FBXL2 protein (Cat# TP306390). The protein was produced from HEK293T cells transfected with FBXL2 cDNA clone (Cat# RC206390) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FBXL2
• Synonyms: FBL2; FBL3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206390 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTTTCTCAAACAATGATGAAGGCCTTATTAACAAAAAGTTACCCAAAGAACTTCTGTTAAGAATAT
  TTTCCTTCTTGGATATAGTAACTTTGTGCCGATGTGCACAGATTTCCAAGGCTTGGAACATCTTAGCCCT
  GGATGGAAGCAACTGGCAAAGAATAGATCTTTTTAACTTTCAAACAGATGTAGAGGGTCGAGTGGTGGAA
  AATATCTCGAAGCGATGCGGTGGATTCCTGAGGAAGCTCAGCTTGCGAGGCTGCATTGGTGTTGGGGATT
  CCTCCTTGAAGACCTTTGCACAGAACTGCCGAAACATTGAACATTTGAACCTCAATGGATGCACAAAAAT
  CACTGACAGCACGTGTTATAGCCTTAGCAGATTCTGTTCCAAGCTGAAACATCTGGATCTGACCTCCTGT
  GTGTCTATTACAAACAGCTCCTTGAAGGGGATCAGTGAGGGCTGCCGAAACCTGGAGTACCTGAACCTCT
  CTTGGTGTGATCAGATCACGAAGGATGGCATCGAGGCACTGGTGCGAGGTTGTCGAGGCCTGAAAGCCCT
  GCTCCTGAGGGGCTGCACACAGTTAGAAGATGAAGCTCTGAAACACATTCAGAATTACTGCCATGAGCTT
  GTGAGCCTCAACTTGCAGTCCTGCTCACGTATCACGGATGAAGGTGTGGTGCAGATATGCAGGGGCTGTC
  ACCGGCTACAGGCTCTCTGCCTTTCGGGTTGCAGCAACCTCACAGATGCCTCTCTTACAGCCCTGGGTTT
  GAACTGTCCGCGACTGCAAATTTTGGAGGCTGCCCGATGCTCCCATTTGACTGACGCAGGTTTTACACTT
  TTAGCTCGGAATTGCCACGAATTGGAGAAGATGGATCTTGAAGAATGCATCCTGATAACCGACAGCACAC
  TCATCCAGCTCTCCATTCACTGTCCTAAACTGCAAGCCCTGAGCCTGTCCCACTGTGAACTCATCACAGA
  TGATGGGATCCTGCACCTGAGCAACAGTACCTGTGGCCATGAGAGGCTGCGGGTACTGGAGTTGGACAAC
  TGCCTCCTCATCACTGATGTGGCCCTGGAACACCTAGAGAACTGCCGAGGCCTGGAGCGCCTCGAGCTGT
  ACGACTGCCAGCAGGTTACCCGTGCAGGCATCAAGCGGATGCGGGCTCAGCTCCCTCATGTCAAAGTCCA
  CGCCTACTTTGCTCCCGTCACCCCACCGACAGCAGTGGCAGGAAGTGGACAGCGACTGTGCAGGTGCTGT
  GTCATTCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206390 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVFSNNDEGLINKKLPKELLLRIFSFLDIVTLCRCAQISKAWNILALDGSNWQRIDLFNFQTDVEGRVVE
  NISKRCGGFLRKLSLRGCIGVGDSSLKTFAQNCRNIEHLNLNGCTKITDSTCYSLSRFCSKLKHLDLTSC
  VSITNSSLKGISEGCRNLEYLNLSWCDQITKDGIEALVRGCRGLKALLLRGCTQLEDEALKHIQNYCHEL
  VSLNLQSCSRITDEGVVQICRGCHRLQALCLSGCSNLTDASLTALGLNCPRLQILEAARCSHLTDAGFTL
  LARNCHELEKMDLEECILITDSTLIQLSIHCPKLQALSLSHCELITDDGILHLSNSTCGHERLRVLELDN
  CLLITDVALEHLENCRGLERLELYDCQQVTRAGIKRMRAQLPHVKVHAYFAPVTPPTAVAGSGQRLCRCC
  VIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012157
• ORF Size: 1269 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012157.1, NM_012157.2, NM_012157.3, NM_012157.4, NP_036289.2
• RefSeq Size: 3000
• RefSeq ORF: 1272
• Locus ID: 25827
• Domains: LRR, F-box, LRR_CC
• Protein Families: Druggable Genome
• MW: 47.1 kDa
• Gene Summary: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]