SCNN1B (NM_000336) Human Tagged ORF Clone

CAT#: RC206580

SCNN1B (Myc-DDK-tagged)-Human sodium channel, nonvoltage-gated 1, beta (SCNN1B)

Product Images

Western blot validation of overexpression lysate (Cat# LY424793) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206580 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SCNN1B
• Synonyms: BESC1; ENaCb; ENaCbeta; LIDLS1; SCNEB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206580 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGTGAAGAAGTACCTGCTGAAGGGCCTGCATCGGCTGCAGAAGGGCCCCGGCTACACGTACAAGG
  AGCTGCTGGTGTGGTACTGCGACAACACCAACACCCACGGCCCCAAGCGCACCATCTGTGAGGGGCCCAA
  GAAGAAAGCCATGTGGTTCCTGCTCACCCTGCTCTTCGCCGCCCTCGTCTGCTGGCAGTGGGGCATCTTC
  ATCAGGACCTACTTGAGCTGGGAGGTCAGCGTCTCCCTCTCCGTAGGCTTCAAGACCATGGACTTCCCTG
  CCGTCACCATCTGCAATGCTAGCCCCTTCAAGTATTCCAAAATCAAGCATTTGCTGAAGGACCTGGATGA
  GCTGATGGAAGCTGTCCTGGAGAGAATCCTGGCTCCTGAGCTAAGCCATGCCAATGCCACCAGGAACCTG
  AACTTCTCCATCTGGAACCACACACCCCTGGTCCTTATTGATGAACGGAACCCCCACCACCCCATGGTCC
  TTGATCTCTTTGGAGACAACCACAATGGCTTAACAAGCAGCTCAGCATCAGAAAAGATCTGTAATGCCCA
  CGGGTGCAAAATGGCCATGAGACTATGTAGCCTCAACAGGACCCAGTGTACCTTCCGGAACTTCACCAGT
  GCTACCCAGGCATTGACAGAGTGGTACATCCTGCAGGCCACCAACATCTTTGCACAGGTGCCACAGCAGG
  AGCTAGTAGAGATGAGCTACCCCGGCGAGCAGATGATCCTGGCCTGCCTATTCGGAGCTGAGCCCTGCAA
  CTACCGGAACTTCACGTCCATCTTCTACCCTCACTATGGCAACTGTTACATCTTCAACTGGGGCATGACA
  GAGAAGGCACTTCCTTCGGCCAACCCTGGAACTGAATTCGGCCTGAAGTTGATCCTGGACATAGGCCAGG
  AAGACTACGTCCCCTTCCTTGCGTCCACGGCCGGGGTCAGGCTGATGCTTCACGAGCAGAGGTCATACCC
  CTTCATCAGAGATGAGGGCATCTACGCCATGTCGGGGACAGAGACGTCCATCGGGGTACTCGTGGACAAG
  CTTCAGCGCATGGGGGAGCCCTACAGCCCGTGCACCGTGAATGGTTCTGAGGTCCCCGTCCAAAACTTCT
  ACAGTGACTACAACACGACCTACTCCATCCAGGCCTGTCTTCGCTCCTGCTTCCAAGACCACATGATCCG
  TAACTGCAACTGTGGCCACTACCTGTACCCACTGCCCCGTGGGGAGAAATACTGCAACAACCGGGACTTC
  CCAGACTGGGCCCATTGCTACTCAGATCTACAGATGAGCGTGGCGCAGAGAGAGACCTGCATTGGCATGT
  GCAAGGAGTCCTGCAATGACACCCAGTACAAGATGACCATCTCCATGGCTGACTGGCCTTCTGAGGCCTC
  CGAGGACTGGATTTTCCACGTCTTGTCTCAGGAGCGGGACCAAAGCACCAATATCACCCTGAGCAGGAAG
  GGAATTGTCAAGCTCAACATCTACTTCCAAGAATTTAACTATCGCACCATTGAAGAATCAGCAGCCAATA
  ACATCGTCTGGCTGCTCTCGAATCTGGGTGGCCAGTTTGGCTTCTGGATGGGGGGCTCTGTGCTGTGCCT
  CATCGAGTTTGGGGAGATCATCATCGACTTTGTGTGGATCACCATCATCAAGCTGGTGGCCTTGGCCAAG
  AGCCTACGGCAGCGGCGAGCCCAAGCCAGCTACGCTGGCCCACCGCCCACCGTGGCCGAGCTGGTGGAGG
  CCCACACCAACTTTGGCTTCCAGCCTGACACGGCCCCCCGCAGCCCCAACACTGGGCCCTACCCCAGTGA
  GCAGGCCCTGCCCATCCCAGGCACCCCGCCCCCCAACTATGACTCCCTGCGTCTGCAGCCGCTGGACGTC
  ATCGAGTCTGACAGTGAGGGTGATGCCATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206580 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHVKKYLLKGLHRLQKGPGYTYKELLVWYCDNTNTHGPKRTICEGPKKKAMWFLLTLLFAALVCWQWGIF
  IRTYLSWEVSVSLSVGFKTMDFPAVTICNASPFKYSKIKHLLKDLDELMEAVLERILAPELSHANATRNL
  NFSIWNHTPLVLIDERNPHHPMVLDLFGDNHNGLTSSSASEKICNAHGCKMAMRLCSLNRTQCTFRNFTS
  ATQALTEWYILQATNIFAQVPQQELVEMSYPGEQMILACLFGAEPCNYRNFTSIFYPHYGNCYIFNWGMT
  EKALPSANPGTEFGLKLILDIGQEDYVPFLASTAGVRLMLHEQRSYPFIRDEGIYAMSGTETSIGVLVDK
  LQRMGEPYSPCTVNGSEVPVQNFYSDYNTTYSIQACLRSCFQDHMIRNCNCGHYLYPLPRGEKYCNNRDF
  PDWAHCYSDLQMSVAQRETCIGMCKESCNDTQYKMTISMADWPSEASEDWIFHVLSQERDQSTNITLSRK
  GIVKLNIYFQEFNYRTIEESAANNIVWLLSNLGGQFGFWMGGSVLCLIEFGEIIIDFVWITIIKLVALAK
  SLRQRRAQASYAGPPPTVAELVEAHTNFGFQPDTAPRSPNTGPYPSEQALPIPGTPPPNYDSLRLQPLDV
  IESDSEGDAI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000336
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000336.1, NM_000336.2, NP_000327.1
• RefSeq Size: 2597 bp
• RefSeq ORF: 1923 bp
• Locus ID: 6338
• Cytogenetics: 16p12.2
• Domains: ASC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Taste transduction
• MW: 72.6 kDa
• Gene Summary: 'Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the beta subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), and Liddle syndrome. [provided by RefSeq, Apr 2009]'