CPN1 (NM_001308) Human Tagged ORF Clone

CAT#: RC206606

CPN1 (Myc-DDK-tagged)-Human carboxypeptidase N, polypeptide 1 (CPN1)

Product Images

Western blot validation of overexpression lysate (Cat# LY420016) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206606 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPN1
• Synonyms: CPN; SCPN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206606 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGACCTGCTCTCAGTCTTCCTCCACCTCCTCCTTCTCTTCAAGTTGGTTGCCCCGGTGACCTTTC
  GCCACCACCGCTATGATGATCTTGTGCGGACGCTGTACAAGGTGCAAAACGAATGCCCCGGCATCACGCG
  GGTCTACAGCATTGGGCGCAGCGTGGAGGGGAGACACCTCTACGTGCTGGAGTTCAGCGACCACCCTGGA
  ATCCACGAGCCCTTGGAACCAGAGGTCAAGTATGTGGGGAACATGCACGGCAACGAAGCGTTGGGCCGCG
  AGCTGATGCTGCAGCTGTCGGAGTTTCTGTGCGAGGAGTTCCGGAACAGGAACCAGCGCATCGTCCAGCT
  CATCCAGGACACGCGCATTCACATCCTGCCATCCATGAACCCCGACGGCTACGAGGTGGCTGCTGCCCAG
  GGCCCAAACAAGCCTGGGTATCTAGTTGGCAGGAACAATGCAAATGGAGTGGACCTGAACCGCAACTTCC
  CTGATCTCAATACCTATATCTACTATAACGAGAAGTACGGAGGCCCCAACCACCACCTGCCCCTTCCAGA
  CAACTGGAAAAGTCAGGTGGAACCCGAGACCCGGGCGGTGATCCGGTGGATGCACTCCTTCAACTTTGTT
  CTTTCAGCCAATCTCCACGGAGGGGCGGTGGTGGCCAATTACCCGTATGACAAGTCCTTTGAGCACCGGG
  TCCGAGGGGTCCGCCGCACCGCCAGCACCCCCACGCCTGACGACAAGCTCTTCCAGAAGCTGGCCAAGGT
  CTACTCCTATGCACATGGATGGATGTTCCAAGGTTGGAACTGCGGAGATTACTTCCCAGATGGCATCACC
  AATGGAGCTTCCTGGTATTCTCTCAGCAAGGGAATGCAAGACTTTAATTATCTCCATACCAACTGCTTTG
  AGATCACGCTGGAACTGAGTTGCGACAAGTTTCCCCCCGAAGAGGAGTTACAGCGGGAGTGGCTGGGTAA
  TCGGGAAGCCCTAATCCAGTTCCTGGAACAGGTTCACCAGGGCATCAAGGGAATGGTGCTTGATGAGAAT
  TACAATAATCTCGCCAATGCTGTCATTTCTGTCAGTGGGATTAACCATGATGTCACTTCAGGTGACCATG
  GTGATTACTTCCGGCTGCTGCTTCCAGGTATCTACACTGTTAGTGCCACAGCACCTGGGTATGACCCAGA
  GACAGTAACTGTGACCGTGGGTCCTGCGGAACCAACGTTGGTTAACTTCCACCTCAAAAGAAGCATCCCT
  CAAGTAAGCCCTGTGAGGAGAGCTCCCAGCAGAAGGCACGGAGTCAGAGCCAAAGTGCAGCCCCAAGCCA
  GAAAGAAAGAAATGGAGATGAGGCAGCTGCAGAGAGGCCCTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206606 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSDLLSVFLHLLLLFKLVAPVTFRHHRYDDLVRTLYKVQNECPGITRVYSIGRSVEGRHLYVLEFSDHPG
  IHEPLEPEVKYVGNMHGNEALGRELMLQLSEFLCEEFRNRNQRIVQLIQDTRIHILPSMNPDGYEVAAAQ
  GPNKPGYLVGRNNANGVDLNRNFPDLNTYIYYNEKYGGPNHHLPLPDNWKSQVEPETRAVIRWMHSFNFV
  LSANLHGGAVVANYPYDKSFEHRVRGVRRTASTPTPDDKLFQKLAKVYSYAHGWMFQGWNCGDYFPDGIT
  NGASWYSLSKGMQDFNYLHTNCFEITLELSCDKFPPEEELQREWLGNREALIQFLEQVHQGIKGMVLDEN
  YNNLANAVISVSGINHDVTSGDHGDYFRLLLPGIYTVSATAPGYDPETVTVTVGPAEPTLVNFHLKRSIP
  QVSPVRRAPSRRHGVRAKVQPQARKKEMEMRQLQRGPA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001308
• ORF Size: 1374 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001308.1, NM_001308.2, NP_001299.1
• RefSeq Size: 1756 bp
• RefSeq ORF: 1377 bp
• Locus ID: 1369
• Cytogenetics: 10q24.2
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 52.3 kDa
• Gene Summary: 'Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq, Jul 2008]'