Muscarinic Acetylcholine Receptor M5 (CHRM5) (NM_012125) Human Tagged ORF Clone

CAT#: RC207196

CHRM5 (Myc-DDK-tagged)-Human cholinergic receptor, muscarinic 5 (CHRM5)

Product Images

Western blot validation of overexpression lysate (Cat# LY402152) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207196 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHRM5
• Synonyms: HM5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207196 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGGGGATTCTTACCACAATGCAACCACCGTCAATGGCACCCCAGTAAATCACCAGCCTTTGGAAC
  GCCACAGGTTGTGGGAAGTCATCACCATTGCAGCTGTGACTGCTGTGGTAAGCCTGATCACCATTGTGGG
  CAATGTCTTGGTCATGATCTCCTTCAAAGTCAACAGCCAGCTCAAGACAGTTAACAACTATTACCTGCTC
  AGCTTAGCCTGTGCAGATCTCATCATTGGAATCTTCTCCATGAACCTCTACACCACCTACATCCTCATGG
  GACGCTGGGCTCTCGGGAGTCTGGCTTGTGACCTTTGGCTTGCACTGGACTACGTGGCCAGCAACGCTTC
  TGTCATGAACCTTCTGGTGATCAGTTTTGACCGTTACTTTTCCATCACAAGACCCTTGACATATCGGGCC
  AAGCGTACTCCGAAAAGGGCTGGCATCATGATTGGCTTGGCCTGGCTGATCTCCTTCATCCTCTGGGCCC
  CAGCAATCCTCTGCTGGCAGTACTTGGTTGGGAAGCGGACAGTTCCACTGGATGAGTGCCAGATCCAGTT
  TCTCTCTGAGCCCACCATCACTTTTGGCACTGCCATTGCTGCCTTCTACATCCCTGTTTCTGTCATGACC
  ATCCTCTACTGTCGAATCTACCGGGAAACAGAGAAGCGAACCAAGGACCTGGCTGACCTCCAGGGTTCTG
  ACTCTGTGACCAAAGCTGAGAAGAGAAAGCCAGCTCATAGGGCTCTGTTCAGATCCTGCTTGCGCTGTCC
  TCGACCCACCCTGGCCCAGCGGGAAAGGAACCAGGCCTCCTGGTCATCCTCCCGCAGGAGCACCTCCACC
  ACTGGGAAGCCATCCCAAGCCACTGGCCCAAGCGCCAATTGGGCCAAAGCTGAGCAGCTCACCACCTGTA
  GCAGCTACCCTTCCTCAGAGGATGAGGACAAGCCCGCCACTGACCCTGTCCTCCAAGTGGTCTACAAGAG
  TCAGGGTAAGGAAAGCCCAGGGGAAGAATTCAGTGCTGAAGAGACTGAGGAAATTTTTGTGAAAGCTGAA
  ACTGAAAAAAGTGACTATGACACCCCAAACTACCTTCTGTCTCCAGCAGCTGCTCATAGACCCAAGAGTC
  AGAAATGTGTGGCCTATAAGTTCCGATTGGTGGTAAAAGCTGACGGGAACCAGGAGACCAACAATGGCTG
  TCACAAGGTGAAAATCATGCCCTGCCCCTTCCCAGTGGCCAAGGAACCTTCAACGAAAGGCCTCAATCCC
  AACCCCAGCCATCAAATGACCAAACGAAAGAGAGTGGTCCTAGTCAAAGAGAGGAAAGCAGCCCAGACAC
  TGAGTGCCATTCTCCTGGCCTTCATCATCACATGGACCCCGTATAACATCATGGTCCTGGTTTCTACCTT
  CTGTGACAAGTGTGTCCCAGTCACCCTGTGGCACTTGGGCTATTGGTTGTGCTATGTCAATAGCACTGTC
  AACCCCATCTGCTATGCCCTCTGCAACAGAACCTTCAGGAAGACCTTTAAGATGCTGCTTCTCTGCCGAT
  GGAAAAAGAAAAAAGTGGAAGAGAAGTTGTACTGGCAGGGGAACAGCAAGCTACCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207196 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGDSYHNATTVNGTPVNHQPLERHRLWEVITIAAVTAVVSLITIVGNVLVMISFKVNSQLKTVNNYYLL
  SLACADLIIGIFSMNLYTTYILMGRWALGSLACDLWLALDYVASNASVMNLLVISFDRYFSITRPLTYRA
  KRTPKRAGIMIGLAWLISFILWAPAILCWQYLVGKRTVPLDECQIQFLSEPTITFGTAIAAFYIPVSVMT
  ILYCRIYRETEKRTKDLADLQGSDSVTKAEKRKPAHRALFRSCLRCPRPTLAQRERNQASWSSSRRSTST
  TGKPSQATGPSANWAKAEQLTTCSSYPSSEDEDKPATDPVLQVVYKSQGKESPGEEFSAEETEEIFVKAE
  TEKSDYDTPNYLLSPAAAHRPKSQKCVAYKFRLVVKADGNQETNNGCHKVKIMPCPFPVAKEPSTKGLNP
  NPSHQMTKRKRVVLVKERKAAQTLSAILLAFIITWTPYNIMVLVSTFCDKCVPVTLWHLGYWLCYVNSTV
  NPICYALCNRTFRKTFKMLLLCRWKKKKVEEKLYWQGNSKLP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_012125
• ORF Size: 1596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012125.1, NM_012125.2, NM_012125.3, NP_036257.1
• RefSeq Size: 3055 bp
• RefSeq ORF: 1599 bp
• Locus ID: 1133
• Cytogenetics: 15q14
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• MW: 60.1 kDa
• Gene Summary: 'The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels. [provided by RefSeq, Jul 2008]'