Muscarinic Acetylcholine Receptor M5 (CHRM5) (NM_012125) Human Untagged Clone

CAT#: SC115522

CHRM5 (untagged)-Human cholinergic receptor, muscarinic 5 (CHRM5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CHRM5
• Synonyms: HM5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012125, RT-PCR generated
  ATGGAAGGGGATTCTTACCACAATGCAACCACCGTCAATGGCACCCCAGTAAATCACCAG
  CCTTTGGAACGCCACAGGTTGTGGGAAGTCATCACCATTGCAGCTGTGACTGCTGTGGTA
  AGCCTGATCACCATTGTGGGCAATGTCTTGGTCATGATCTCCTTCAAAGTCAACAGCCAG
  CTCAAGACAGTTAACAACTATTACCTGCTCAGCTTAGCCTGTGCAGATCTCATCATTGGA
  ATCTTCTCCATGAACCTCTACACCACCTACATCCTCATGGGACGCTGGGCTCTCGGGAGT
  CTGGCTTGTGACCTTTGGCTTGCACTGGACTACGTGGCCAGCAACGCTTCTGTCATGAAC
  CTTCTGGTGATCAGTTTTGACCGTTACTTTTCCATCACAAGACCCTTGACATATCGGGCC
  AAGCGTACTCCGAAAAGGGCTGGCATCATGATTGGCTTGGCCTGGCTGATCTCCTTCATC
  CTCTGGGCCCCAGCAATCCTCTGCTGGCAGTACTTGGTTGGGAAGCGGACAGTTCCACTG
  GATGAGTGCCAGATCCAGTTTCTCTCTGAGCCCACCATCACTTTTGGCACTGCCATTGCT
  GCCTTCTACATCCCTGTTTCTGTCATGACCATCCTCTACTGTCGAATCTACCGGGAAACA
  GAGAAGCGAACCAAGGACCTGGCTGACCTCCAGGGTTCTGACTCTGTGACCAAAGCTGAG
  AAGAGAAAGCCAGCTCATAGGGCTCTGTTCAGATCCTGCTTGCGCTGTCCTCGACCCACC
  CTGGCCCAGCGGGAAAGGAACCAGGCCTCCTGGTCATCCTCCCGCAGGAGCACCTCCACC
  ACTGGGAAGCCATCCCAAGCCACTGGCCCAAGCGCCAATTGGGCCAAAGCTGAGCAGCTC
  ACCACCTGTAGCAGCTACCCTTCCTCAGAGGATGAGGACAAGCCCGCCACTGACCCTGTC
  CTCCAAGTGGTCTACAAGAGTCAGGGTAAGGAAAGCCCAGGGGAAGAATTCAGTGCTGAA
  GAGACTGAGGAAACTTTTGTGAAAGCTGAAACTGAAAAAAGTGACTATGACACCCCAAAC
  TACCTTCTGTCTCCAGCAGCTGCTCATAGACCCAAGAGTCAGAAATGTGTGGCCTATAAG
  TTCCGATTGGTGGTAAAAGCTGACGGGAACCAGGAGACCAACAATGGCTGTCACAAGGTG
  AAAATCATGCCCTGCCCCTTCCCAGTGGCCAAGGAACCTTCAACGAAAGGCCTCAATCCC
  AACCCCAGCCATCAAATGACCAAACGAAAGAGAGTGGTCCTAGTCAAAGAGAGGAAAGCA
  GCCCAGACACTGAGTGCCATTCTCCTGGCCTTCATCATCACATGGACCCCGTATAACATC
  ATGGTCCTGGTTTCTACCTTCTGTGACAAGTGTGTCCCAGTCACCCTGTGGCACTTGGGC
  TATTGGTTGTGCTATGTCAATAGCACTGTCAACCCCATCTGCTATGCCCTCTGCAACAGA
  ACCTTCAGGAAGACCTTTAAGATGCTGCTTCTCTGCCGATGGAAAAAGAAAAAAGTGGAA
  GAGAAGTTGTACTGGCAGGGGAACAGCAAGCTACCCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012125 unedited
  TTCCCCGCATTTTCAGGATTTGTNATACGATTTATATAGGCGGCCGCCAGATGTGATGGA
  ATCTGCAGAATTCGGCTTATGGAAGGGATTCTTACCACAATGCAACCACCGTCAATGGCA
  CCCCAGTAAACACCAGCCTTTGGAACGCCACAGGTTGTGGGAAGTCATCACCATTGCAGC
  TGTGACTGCTGTGGTAAGCCTGATCACCATTGTGGGCAATGTCTTGGTCATGATCTCCTT
  CAAAGTCAACAGCCAGCTCAAGACAGTTAACAACTATTACCTGCTCAGCTTAGCCTGTGC
  AGATCTCATCATTGGAATCTTCTCCATGAACCTCTACACCACCTACATCCTCATGGGACG
  CTGGGCTCTCGGGAGTCTGGCTTGTGACCTTTGGCTTGCACTGGACTACGTGGCCAGCAA
  CGCTTCTGTCATGAACCTTCTGGTGATCAGTTTTGACCGTTACTTTTCCATCACAAGACC
  CTTGACATATCGGGCCAAGCGTACTCCGAAAAGGGCTGGCATCATGATTGGCTTGGCCTG
  GCTGATCTCCTTCATCCTCTGGGCCCCAGCAATCCTCTGCTGGCAGTACTTGGTTGGGAA
  GCGGACAGTTCCACTGGATGAGTGCCAGATCCAGTTTCTCTCTGAGCCCACCATCACTTT
  TGGCACTGCCATTGCTGCCTTCTACATCCCTGTTTCTGTCATGACCATCCTCTACTGTCG
  AATCTACCGGGAAACAGAGAAGCGAACCAAGGACCTGGCTGACCTCCAGGGTTCTGACTC
  TGTGACCAAAGCTGAGAAGAGAAAGCCAGCTCATAGGGCTCTGTTCAGATCCTGCTTGCG
  CTGTCCTCGACCCACCCTGGCCCAGCGGGNAAAGAAC
  
• Restriction Sites: Please inquire
• ACCN: NM_012125
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012125.2, NP_036257.1
• RefSeq Size: 2699 bp
• RefSeq ORF: 1599 bp
• Locus ID: 1133
• Cytogenetics: 15q14
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Neuroactive ligand-receptor interaction, Regulation of actin cytoskeleton
• Gene Summary: 'The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, stimulation of this receptor is known to increase cyclic AMP levels. [provided by RefSeq, Jul 2008]'