PNK (PNKP) (NM_007254) Human Tagged ORF Clone

CAT#: RC207551

PNKP (Myc-DDK-tagged)-Human polynucleotide kinase 3'-phosphatase (PNKP)

Product Images

Western blot validation of overexpression lysate (Cat# LY416093) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207551 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified PNKP protein (Cat# TP307551). The protein was produced from HEK293T cells transfected with PNKP cDNA clone (Cat# RC207551) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PNKP
• Synonyms: AOA4; EIEE10; MCSZ; PNK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207551 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCGAGGTGGAGGCCCCGGGCCGCTTGTGGCTCGAGAGCCCCCCTGGGGGAGCGCCCCCCATCTTCC
  TGCCCTCGGACGGGCAAGCCCTGGTCCTGGGCAGGGGACCCCTGACCCAGGTTACGGACCGGAAGTGCTC
  CAGAACTCAAGTGGAGCTGGTCGCAGATCCTGAGACCCGGACAGTGGCAGTGAAACAGCTGGGAGTTAAC
  CCCTCAACTACCGGGACCCAGGAGTTGAAGCCGGGGTTGGAGGGCTCTCTGGGGGTGGGGGACACACTGT
  ATTTGGTCAATGGCCTCCACCCACTGACCCTGCGCTGGGAAGAGACCCGCACACCAGAATCCCAGCCAGA
  TACTCCGCCTGGCACCCCTCTGGTGTCCCAAGATGAGAAGAGAGATGCTGAGCTGCCGAAGAAGCGTATG
  CGGAAGTCAAACCCCGGCTGGGAGAACTTGGAGAAGTTGCTAGTGTTCACCGCAGCTGGGGTGAAACCCC
  AGGGCAAGGTGGCTGGCTTTGATCTGGACGGGACGCTCATCACCACACGCTCTGGGAAGGTCTTTCCCAC
  TGGCCCCAGTGACTGGAGGATCTTGTACCCAGAGATTCCCCGTAAGCTCCGAGAGCTGGAAGCCGAGGGC
  TACAAGCTGGTGATCTTCACCAACCAGATGAGCATCGGGCGCGGGAAGCTGCCAGCCGAGGAGTTCAAGG
  CCAAGGTGGAGGCTGTGGTGGAGAAGCTGGGGGTCCCCTTCCAGGTGCTGGTGGCCACGCACGCAGGCTT
  GTACCGGAAGCCGGTGACGGGCATGTGGGACCATCTGCAGGAGCAGGCCAACGACGGCACGCCCATATCC
  ATCGGGGACAGCATCTTTGTGGGAGACGCAGCCGGACGCCCGGCCAACTGGGCCCCGGGGCGGAAGAAGA
  AAGACTTCTCCTGCGCCGATCGCCTGTTTGCCCTCAACCTTGGCCTGCCCTTCGCCACGCCTGAGGAGTT
  CTTTCTCAAGTGGCCAGCAGCCGGCTTCGAGCTCCCAGCCTTTGATCCGAGGACTGTCTCCCGCTCAGGG
  CCTCTCTGCCTCCCCGAGTCCAGGGCCCTCCTGAGCGCCAGCCCGGAGGTGGTTGTCGCAGTGGGATTCC
  CTGGGGCCGGGAAGTCCACCTTTCTCAAGAAGCACCTCGTGTCGGCCGGATATGTCCACGTGAACAGGGA
  CACGCTAGGCTCCTGGCAGCGCTGTGTGACCACGTGTGAGACAGCCCTGAAGCAAGGGAAACGGGTCGCC
  ATCGACAACACAAACCCAGACGCCGCGAGCCGCGCCAGGTACGTCCAGTGTGCCCGAGCCGCGGGCGTCC
  CCTGCCGCTGCTTCCTCTTCACCGCCACTCTGGAGCAGGCGCGCCACAACAACCGGTTTCGAGAGATGAC
  GGACTCCTCTCATATCCCCGTGTCAGACATGGTCATGTATGGCTACAGGAAGCAGTTCGAGGCCCCAACG
  CTGGCTGAAGGCTTCTCTGCCATCCTGGAGATCCCGTTCCGGCTATGGGTGGAGCCGAGGCTGGGGCGGC
  TGTACTGCCAGTTCTCCGAGGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207551 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGEVEAPGRLWLESPPGGAPPIFLPSDGQALVLGRGPLTQVTDRKCSRTQVELVADPETRTVAVKQLGVN
  PSTTGTQELKPGLEGSLGVGDTLYLVNGLHPLTLRWEETRTPESQPDTPPGTPLVSQDEKRDAELPKKRM
  RKSNPGWENLEKLLVFTAAGVKPQGKVAGFDLDGTLITTRSGKVFPTGPSDWRILYPEIPRKLRELEAEG
  YKLVIFTNQMSIGRGKLPAEEFKAKVEAVVEKLGVPFQVLVATHAGLYRKPVTGMWDHLQEQANDGTPIS
  IGDSIFVGDAAGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDPRTVSRSG
  PLCLPESRALLSASPEVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRDTLGSWQRCVTTCETALKQGKRVA
  IDNTNPDAASRARYVQCARAAGVPCRCFLFTATLEQARHNNRFREMTDSSHIPVSDMVMYGYRKQFEAPT
  LAEGFSAILEIPFRLWVEPRLGRLYCQFSEG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007254
• ORF Size: 1563 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007254.1, NM_007254.2, NM_007254.3, NP_009185.2
• RefSeq Size: 1721
• RefSeq ORF: 1566
• Locus ID: 11284
• Protein Families: Druggable Genome, Phosphatase
• MW: 57.1 kDa
• Gene Summary: This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay. [provided by RefSeq, Sep 2010]